Removal of phenolic compounds from wastewaters using soybean peroxidase
- McGill Univ., Montreal, Quebec (Canada). Dept. of Civil Engineering and Applied Mechanics
Toxic and odiferous phenolic compounds are present in wastewaters generated by a variety of industries including petroleum refining, plastics, resins, textiles, and iron and steel manufacturing among others. Due to its commercial availability in purified form, its useful presence in raw plant material, and its proven ability to remove a variety of phenolic contaminants from wastewaters over a wide range of pH and temperature, horseradish peroxidase (HRP) appears to be the peroxidase enzyme of choice in enzymatic wastewater treatment studies. Problems with HRP catalyzed phenol removal, however, include the formation of toxic soluble reaction by-products, the cost of the enzyme, and costs associated with disposal of the phenolic precipitate generated. Enzyme costs are incurred because the enzyme is inactivated during the phenol removal process by various side reactions. While recent work has shown that enzyme inactivation can be reduced using chemical additives, the problem of enzyme cost could be circumvented by using a less expensive source of enzyme. In 1991, the seed coat of the soybean was identified as a very rich source of peroxidase enzyme. Since the seed coat of the soybean is a waste product of the soybean food industry, soybean peroxidase (SBP) has the potential of being a cost effective alternative to HRP in wastewater treatment. In this study, SBP is characterized in terms of its catalytic activity, its stability, and its ability to promote removal of phenolic compounds from synthetic wastewaters. Results obtained are discussed and compared to similar investigations using HRP.
- OSTI ID:
- 395321
- Report Number(s):
- CONF-9505206-; TRN: IM9648%%444
- Resource Relation:
- Conference: 50. Purdue industrial waste conference, W. Lafayette, IN (United States), 8-10 May 1995; Other Information: PBD: 1996; Related Information: Is Part Of Proceedings of the 50. industrial waste conference; Wukasch, R.F. [ed.]; PB: 861 p.
- Country of Publication:
- United States
- Language:
- English
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