Metallothionein (MT) induction in channel catfish following treatment with therapeutic levels of copper sulfate
- Univ. of Mississippi, University, MS (United States)
- National Biological Survey, Stuttgart, AR (United States)
Copper sulfate is regularly used in the aquaculture industry for the treatment of various ectoparasitic infestations. Male and female Channel catfish (Ictlurus punctatus) were treated for 8 weeks with three therapeutic doses of copper sulfate (1.7, 2.7 and 3.6 mg/L). Liver, gill, spleen, and gonads were dissected from 6 fish sampled at two week intervals. Samples were frozen and will be analyzed for MT in each tissue. Total MT will be measured by ELISA using antibodies raised against the first 10 amino acid residues which are conserved in fish. MT mRNA will also be measured in each tissue by the Ribonuclease Protection Assay using winter flounder CDNA and compared to protein measurements during the exposure period. MT levels also will be compared to total copper accumulation levels in each fish.
- OSTI ID:
- 392325
- Report Number(s):
- CONF-9511137-; ISBN 1-880611-03-1; TRN: IM9647%%179
- Resource Relation:
- Conference: 2. Society of Environmental Toxicology and Chemistry (SETAC) world conference, Vancouver (Canada), 5-9 Nov 1995; Other Information: PBD: 1995; Related Information: Is Part Of Second SETAC world congress (16. annual meeting): Abstract book. Global environmental protection: Science, politics, and common sense; PB: 378 p.
- Country of Publication:
- United States
- Language:
- English
Similar Records
Copper and zinc effects on hematologic system and immune responses in channel catfish, Ictalurus punctatus
Copper accumulation in channel catfish (Ictalurus punctatus) exposed to water borne copper sulfate