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Title: Cloning of a cDNA encoding cellobiose dehydrogenase, a hemoflavoenzyme from Phanerochaete chrysosporium

Abstract

Cellobiose dehydrogenase (CDH) is an extracellular hemoflovoenzyme produced by cellulose-degrading cultures of Phanerochaete Chrysoporium. In this report, a 2,4-kb cDNA encoding CDH was isolated by screening an expression library of P. chrysoporium OGC101 with a CDH-specific polychlonal antibody. Little sequence similarity to hemofloenzymens is found. CDH binds to cellulose similarly to cellulases. However little sequence similarity is observed with the conserved cellulose-binding sequences of cellulases, suggesting CDH might possess a specific sequence for cellulose binding which is different from that of cellulases. Other test indicate CDH mRNA is produced only in cellulose-grown cells, suggesting CDH expression is regulated at the transcription level by either cellulose or one of its degradation products. The presence of a signal gen for CDH in P. chrysosporium is also suggested.

Authors:
;  [1];  [2]
  1. Oregon Graduate Institute of Science & Technology, Portland, OR (United States)
  2. Oregon Regional Primate Research Center, Beaverton, OR (United States)
Publication Date:
OSTI Identifier:
379165
Resource Type:
Journal Article
Journal Name:
Applied and Environmental Microbiology
Additional Journal Information:
Journal Volume: 62; Journal Issue: 4; Other Information: PBD: Apr 1996
Country of Publication:
United States
Language:
English
Subject:
55 BIOLOGY AND MEDICINE, BASIC STUDIES; PHANEROCHAETE; BIOCHEMISTRY; OXIDOREDUCTASES; GENETIC CONTROL; DNA; CLONING; CELLOBIOSE

Citation Formats

Li, B, Renganathan, V, and Nagalla, R. Cloning of a cDNA encoding cellobiose dehydrogenase, a hemoflavoenzyme from Phanerochaete chrysosporium. United States: N. p., 1996. Web.
Li, B, Renganathan, V, & Nagalla, R. Cloning of a cDNA encoding cellobiose dehydrogenase, a hemoflavoenzyme from Phanerochaete chrysosporium. United States.
Li, B, Renganathan, V, and Nagalla, R. Mon . "Cloning of a cDNA encoding cellobiose dehydrogenase, a hemoflavoenzyme from Phanerochaete chrysosporium". United States.
@article{osti_379165,
title = {Cloning of a cDNA encoding cellobiose dehydrogenase, a hemoflavoenzyme from Phanerochaete chrysosporium},
author = {Li, B and Renganathan, V and Nagalla, R},
abstractNote = {Cellobiose dehydrogenase (CDH) is an extracellular hemoflovoenzyme produced by cellulose-degrading cultures of Phanerochaete Chrysoporium. In this report, a 2,4-kb cDNA encoding CDH was isolated by screening an expression library of P. chrysoporium OGC101 with a CDH-specific polychlonal antibody. Little sequence similarity to hemofloenzymens is found. CDH binds to cellulose similarly to cellulases. However little sequence similarity is observed with the conserved cellulose-binding sequences of cellulases, suggesting CDH might possess a specific sequence for cellulose binding which is different from that of cellulases. Other test indicate CDH mRNA is produced only in cellulose-grown cells, suggesting CDH expression is regulated at the transcription level by either cellulose or one of its degradation products. The presence of a signal gen for CDH in P. chrysosporium is also suggested.},
doi = {},
url = {https://www.osti.gov/biblio/379165}, journal = {Applied and Environmental Microbiology},
number = 4,
volume = 62,
place = {United States},
year = {1996},
month = {4}
}