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Title: Transcriptional control of ADH genes in the xylose-fermenting yeast Pichia stipitis

Abstract

The authors studied the expression of the genes encoding group 1 alcohol dehydrogenases (PsADH1 and PsADH2) in the xylose-fermenting yeast Pichia stipitis CBS 6054. The cells expressed PsADH1 approximately 10 times higher under oxygen-limited conditions than under fully aerobic conditions when cultivated on xylose. Transcripts of PsADH2 were not detectable under either aeration condition. The authors used a PsADH1::lacZ fusion to monitor PsADH1 expression and found that expression increased as oxygen decreased. The level of PsADH1 transcript was expressed about 10-fold in cells grown in the presence of heme under oxygen-limited conditions. Concomitantly with the induction of PsADH1, PsCYC1 expression was regressed. These results indicate that oxygen availability regulates PsADH1 expression and that regulation may be mediated by heme. The regulation of PsADH2 expression was also examined in other genetic backgrounds. Disruption of PsADH1 dramatically increased PsADH2 expression on nonfermentable carbon sources under fully aerobic conditions, indicating that the expression of PsADH2 is subject to feedback regulation under these conditions.

Authors:
;  [1]
  1. Forest Service, Madison, WI (United States). Forest Products Lab.
Publication Date:
Sponsoring Org.:
National Renewable Energy Lab., Golden, CO (United States); Department of Agriculture, Washington, DC (United States)
OSTI Identifier:
354324
Resource Type:
Journal Article
Journal Name:
Applied and Environmental Microbiology
Additional Journal Information:
Journal Volume: 65; Journal Issue: 6; Other Information: PBD: Jun 1999
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; XYLOSE; FERMENTATION; YEASTS; TRANSCRIPTION; GENE REGULATION; ALCOHOL DEHYDROGENASE

Citation Formats

Cho, J Y, Jeffries, T W, and Univ. of Wisconsin, Madison, WI. Transcriptional control of ADH genes in the xylose-fermenting yeast Pichia stipitis. United States: N. p., 1999. Web.
Cho, J Y, Jeffries, T W, & Univ. of Wisconsin, Madison, WI. Transcriptional control of ADH genes in the xylose-fermenting yeast Pichia stipitis. United States.
Cho, J Y, Jeffries, T W, and Univ. of Wisconsin, Madison, WI. Tue . "Transcriptional control of ADH genes in the xylose-fermenting yeast Pichia stipitis". United States.
@article{osti_354324,
title = {Transcriptional control of ADH genes in the xylose-fermenting yeast Pichia stipitis},
author = {Cho, J Y and Jeffries, T W and Univ. of Wisconsin, Madison, WI},
abstractNote = {The authors studied the expression of the genes encoding group 1 alcohol dehydrogenases (PsADH1 and PsADH2) in the xylose-fermenting yeast Pichia stipitis CBS 6054. The cells expressed PsADH1 approximately 10 times higher under oxygen-limited conditions than under fully aerobic conditions when cultivated on xylose. Transcripts of PsADH2 were not detectable under either aeration condition. The authors used a PsADH1::lacZ fusion to monitor PsADH1 expression and found that expression increased as oxygen decreased. The level of PsADH1 transcript was expressed about 10-fold in cells grown in the presence of heme under oxygen-limited conditions. Concomitantly with the induction of PsADH1, PsCYC1 expression was regressed. These results indicate that oxygen availability regulates PsADH1 expression and that regulation may be mediated by heme. The regulation of PsADH2 expression was also examined in other genetic backgrounds. Disruption of PsADH1 dramatically increased PsADH2 expression on nonfermentable carbon sources under fully aerobic conditions, indicating that the expression of PsADH2 is subject to feedback regulation under these conditions.},
doi = {},
journal = {Applied and Environmental Microbiology},
number = 6,
volume = 65,
place = {United States},
year = {1999},
month = {6}
}