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Title: Reductive dechlorination of tetrachloroethene to cis-1,2-dichloroethene by a thermophilic anaerobic enrichment culture

Abstract

Thermophilic anaerobic biodegradation of tetrachloroethene (PCE) was investigated with various inocula from geothermal and nongeothermal areas. Only polluted harbor sediment resulted in a stable enrichment culture that converted PCE via trichloroethene to cis-1,2-dichloroethene at the optimum temperature of 60 to 65 C. After several transfers, methanogens were eliminated from the culture. Dechlorination was supported by lactate, pyruvate, fructose, fumarate, and malate as electron donor but not by H{sub 2}, formate, or acetate. Fumarate and L-malate led to the highest dechlorination rate. In the absence of PCE, fumerate was fermented to acetate, H{sub 2}, CO{sub 2}, and succinate. With PCE, less H{sub 2} was formed, suggesting that PCE competed for the reducing equivalents leading to H{sub 2}. PCE dechlorination, apparently, was not outcompeted by fumarate as electron acceptor. At the optimum dissolved PCE concentration of {approximately}60 {micro}M, a high dechlorination rate of 1.1 {micro}mol h{sup {minus}1} mg{sup {minus}1} (dry weight) was found, which indicates that the dechlorination is not a cometabolic activity. Microscopic analysis of the fumarate-grown culture showed the dominance of a long thin rod. Molecular analysis, however, indicated the presence of two dominant species, both belonging to the low-G+C gram positives. The highest similarity was found with the genusmore » Dehalobacter (90%), represented by the halorespiring organism Dehalobacter restrictus, and with the genus Desulfotomaculum (86%).« less

Authors:
; ; ; ;  [1];  [2]
  1. Wageningen Univ. (Netherlands). Lab. of Microbiology
  2. GBF, Braunschweig (Germany). Dept. of Microbiology
Publication Date:
OSTI Identifier:
354321
Resource Type:
Journal Article
Journal Name:
Applied and Environmental Microbiology
Additional Journal Information:
Journal Volume: 65; Journal Issue: 6; Other Information: PBD: Jun 1999
Country of Publication:
United States
Language:
English
Subject:
54 ENVIRONMENTAL SCIENCES; BIODEGRADATION; REMEDIAL ACTION; CHLORINATED ALIPHATIC HYDROCARBONS; DECHLORINATION; ANAEROBIC CONDITIONS

Citation Formats

Kengen, S W.M., Breidenbach, C G, Stams, A J.M., Schraa, G, Vos, W.M. de, and Felske, A. Reductive dechlorination of tetrachloroethene to cis-1,2-dichloroethene by a thermophilic anaerobic enrichment culture. United States: N. p., 1999. Web.
Kengen, S W.M., Breidenbach, C G, Stams, A J.M., Schraa, G, Vos, W.M. de, & Felske, A. Reductive dechlorination of tetrachloroethene to cis-1,2-dichloroethene by a thermophilic anaerobic enrichment culture. United States.
Kengen, S W.M., Breidenbach, C G, Stams, A J.M., Schraa, G, Vos, W.M. de, and Felske, A. 1999. "Reductive dechlorination of tetrachloroethene to cis-1,2-dichloroethene by a thermophilic anaerobic enrichment culture". United States.
@article{osti_354321,
title = {Reductive dechlorination of tetrachloroethene to cis-1,2-dichloroethene by a thermophilic anaerobic enrichment culture},
author = {Kengen, S W.M. and Breidenbach, C G and Stams, A J.M. and Schraa, G and Vos, W.M. de and Felske, A},
abstractNote = {Thermophilic anaerobic biodegradation of tetrachloroethene (PCE) was investigated with various inocula from geothermal and nongeothermal areas. Only polluted harbor sediment resulted in a stable enrichment culture that converted PCE via trichloroethene to cis-1,2-dichloroethene at the optimum temperature of 60 to 65 C. After several transfers, methanogens were eliminated from the culture. Dechlorination was supported by lactate, pyruvate, fructose, fumarate, and malate as electron donor but not by H{sub 2}, formate, or acetate. Fumarate and L-malate led to the highest dechlorination rate. In the absence of PCE, fumerate was fermented to acetate, H{sub 2}, CO{sub 2}, and succinate. With PCE, less H{sub 2} was formed, suggesting that PCE competed for the reducing equivalents leading to H{sub 2}. PCE dechlorination, apparently, was not outcompeted by fumarate as electron acceptor. At the optimum dissolved PCE concentration of {approximately}60 {micro}M, a high dechlorination rate of 1.1 {micro}mol h{sup {minus}1} mg{sup {minus}1} (dry weight) was found, which indicates that the dechlorination is not a cometabolic activity. Microscopic analysis of the fumarate-grown culture showed the dominance of a long thin rod. Molecular analysis, however, indicated the presence of two dominant species, both belonging to the low-G+C gram positives. The highest similarity was found with the genus Dehalobacter (90%), represented by the halorespiring organism Dehalobacter restrictus, and with the genus Desulfotomaculum (86%).},
doi = {},
url = {https://www.osti.gov/biblio/354321}, journal = {Applied and Environmental Microbiology},
number = 6,
volume = 65,
place = {United States},
year = {Tue Jun 01 00:00:00 EDT 1999},
month = {Tue Jun 01 00:00:00 EDT 1999}
}