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Title: Activation and inactivation of Pseudomonas stutzeri methylbenzene catabolism pathways mediated by a transposable element

Abstract

The arrangement of the genes involved in o-xylene, m-xylene, and p-xylene catabolism was investigated in three Pseudomonas stutzeri strains: the wild-type strain OX1, which is able to grow on o-xylene but not on the meta and para isomers; the mutant M1, which grows on m-xylene and p-xylene but is unable to utilize the ortho isomer; and the revertant R1, which can utilize all the three isomers of xylene. A 3-kb insertion sequence (IS) termed ISPs1, which inactivates the m-xylene and p-xylene catabolic pathway in P. stutzeri OX1 and the o-xylene catabolic genes in P. stutzeri M1, was detected. No IS was detected in the corresponding catabolic regions of the P. stutzeri R1 genome. ISPs1 is present in several copies in the genomes of the three strains. It is flanked by 24-bp imperfect inverted repeats, causes the direct duplication of 8 bp in the target DNA, and seems to be related to the ISL3 family.

Authors:
; ; ;  [1]
  1. Univ. Degli Studi di Milano, Milan (Italy). Dipt. di Genetica e di Biologia dei Microrganismi
Publication Date:
OSTI Identifier:
346828
Resource Type:
Journal Article
Resource Relation:
Journal Name: Applied and Environmental Microbiology; Journal Volume: 65; Journal Issue: 5; Other Information: PBD: May 1999
Country of Publication:
United States
Language:
English
Subject:
54 ENVIRONMENTAL SCIENCES; BIODEGRADATION; REMEDIAL ACTION; TOLUENE; PSEUDOMONAS; CATABOLISM; BIOLOGICAL PATHWAYS

Citation Formats

Bolognese, F., Di Lecce, C., Galli, E., and Barbieri, P.. Activation and inactivation of Pseudomonas stutzeri methylbenzene catabolism pathways mediated by a transposable element. United States: N. p., 1999. Web.
Bolognese, F., Di Lecce, C., Galli, E., & Barbieri, P.. Activation and inactivation of Pseudomonas stutzeri methylbenzene catabolism pathways mediated by a transposable element. United States.
Bolognese, F., Di Lecce, C., Galli, E., and Barbieri, P.. Sat . "Activation and inactivation of Pseudomonas stutzeri methylbenzene catabolism pathways mediated by a transposable element". United States.
@article{osti_346828,
title = {Activation and inactivation of Pseudomonas stutzeri methylbenzene catabolism pathways mediated by a transposable element},
author = {Bolognese, F. and Di Lecce, C. and Galli, E. and Barbieri, P.},
abstractNote = {The arrangement of the genes involved in o-xylene, m-xylene, and p-xylene catabolism was investigated in three Pseudomonas stutzeri strains: the wild-type strain OX1, which is able to grow on o-xylene but not on the meta and para isomers; the mutant M1, which grows on m-xylene and p-xylene but is unable to utilize the ortho isomer; and the revertant R1, which can utilize all the three isomers of xylene. A 3-kb insertion sequence (IS) termed ISPs1, which inactivates the m-xylene and p-xylene catabolic pathway in P. stutzeri OX1 and the o-xylene catabolic genes in P. stutzeri M1, was detected. No IS was detected in the corresponding catabolic regions of the P. stutzeri R1 genome. ISPs1 is present in several copies in the genomes of the three strains. It is flanked by 24-bp imperfect inverted repeats, causes the direct duplication of 8 bp in the target DNA, and seems to be related to the ISL3 family.},
doi = {},
journal = {Applied and Environmental Microbiology},
number = 5,
volume = 65,
place = {United States},
year = {Sat May 01 00:00:00 EDT 1999},
month = {Sat May 01 00:00:00 EDT 1999}
}