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Title: Regulation of ferulate-5-hydroxylase expression in Arabidopsis in the context of sinapate ester biosynthesis

Abstract

Sinapic acid is an intermediate in syringyl lignin biosynthesis in angiosperms, and in some taxa serves as a precursor for soluble secondary metabolites. The biosynthesis and accumulation of the sinapate esters sinapoylglucose, sinapolymalate, and sinapolycholine are developmentally regulated in Arabidopsis and other members of the Brassicaceae. The FAH1 locus of Arabidopsis encodes the enzyme ferulate-5-hydroxylase (F5H), which catalyzes the rate-limiting step in syringyl lignin biosynthesis and is required for the production of sinapate esters. Here the authors show that F5H expression parallels sinapate ester accumulation in developing siliques and seedlings, but is not rate limiting for their biosynthesis. RNA gel-blot analysis indicated that the tissue-specific and developmentally regulated expression of F5H mRNA is distinct from that of other phenylpropanoid genes. Efforts to identify constructs capable of complementing the sinapate ester-deficient phenotype of fah1 mutants demonstrated that F5H expression in leaves is dependent on sequences 3{prime} of the F5H coding region. In contrast, the positive regulatory function of the downstream region is not required for F5H transcript or sinapolycholine accumulation in embryos.

Authors:
; ; ;  [1]
  1. Purdue Univ., West Lafayette, IN (United States). Dept. of Biochemistry
Publication Date:
OSTI Identifier:
305472
Resource Type:
Journal Article
Journal Name:
Plant Physiology (Bethesda)
Additional Journal Information:
Journal Volume: 119; Journal Issue: 1; Other Information: PBD: Jan 1999
Country of Publication:
United States
Language:
English
Subject:
55 BIOLOGY AND MEDICINE, BASIC STUDIES; ARABIDOPSIS; GENE REGULATION; HYDROXYLASES; BIOSYNTHESIS; LIGNIN; ESTERS

Citation Formats

Ruegger, M, Meyer, K, Cusumano, J C, and Chapple, C. Regulation of ferulate-5-hydroxylase expression in Arabidopsis in the context of sinapate ester biosynthesis. United States: N. p., 1999. Web. doi:10.1104/pp.119.1.101.
Ruegger, M, Meyer, K, Cusumano, J C, & Chapple, C. Regulation of ferulate-5-hydroxylase expression in Arabidopsis in the context of sinapate ester biosynthesis. United States. doi:10.1104/pp.119.1.101.
Ruegger, M, Meyer, K, Cusumano, J C, and Chapple, C. Fri . "Regulation of ferulate-5-hydroxylase expression in Arabidopsis in the context of sinapate ester biosynthesis". United States. doi:10.1104/pp.119.1.101.
@article{osti_305472,
title = {Regulation of ferulate-5-hydroxylase expression in Arabidopsis in the context of sinapate ester biosynthesis},
author = {Ruegger, M and Meyer, K and Cusumano, J C and Chapple, C},
abstractNote = {Sinapic acid is an intermediate in syringyl lignin biosynthesis in angiosperms, and in some taxa serves as a precursor for soluble secondary metabolites. The biosynthesis and accumulation of the sinapate esters sinapoylglucose, sinapolymalate, and sinapolycholine are developmentally regulated in Arabidopsis and other members of the Brassicaceae. The FAH1 locus of Arabidopsis encodes the enzyme ferulate-5-hydroxylase (F5H), which catalyzes the rate-limiting step in syringyl lignin biosynthesis and is required for the production of sinapate esters. Here the authors show that F5H expression parallels sinapate ester accumulation in developing siliques and seedlings, but is not rate limiting for their biosynthesis. RNA gel-blot analysis indicated that the tissue-specific and developmentally regulated expression of F5H mRNA is distinct from that of other phenylpropanoid genes. Efforts to identify constructs capable of complementing the sinapate ester-deficient phenotype of fah1 mutants demonstrated that F5H expression in leaves is dependent on sequences 3{prime} of the F5H coding region. In contrast, the positive regulatory function of the downstream region is not required for F5H transcript or sinapolycholine accumulation in embryos.},
doi = {10.1104/pp.119.1.101},
journal = {Plant Physiology (Bethesda)},
number = 1,
volume = 119,
place = {United States},
year = {1999},
month = {1}
}