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Title: New fluorescence methodology for detecting DNA adducts. Final progress report, May 1, 1991--November 30, 1994

Abstract

A new reagent, {open_quotes}BO-IMI{close_quotes}, has been developed that achieves, for the first time, single step, phosphate specific fluorescence labeling under aqueous conditions. Both 3{prime} and 5{prime} mononucleotides, including representative DNA adducts can be labeled. Included in this technique is a convenient procedure for postlabeling sample cleanup, leading to a practical detection of the products by capillary electrophoresis with laser fluorescence detection (CE-LIF). We consider that this new method will have a significant impact on the measurement of DNA adducts in human samples. This work was largely accomplished in the second half of our project. In the first half, we set up a new way to isolate DNA nucleotides from blood, worked with an initial, less specific technique for labeling DNA adducts, compared ionizing radiation vs oxidative damage to fluorescein labeled deoxyadenylic acid, and set up a capillary electrophoresis laser fluorescence detection system.

Authors:
Publication Date:
Research Org.:
Northeastern Univ., Boston, MA (United States)
Sponsoring Org.:
USDOE, Washington, DC (United States)
OSTI Identifier:
29367
Report Number(s):
DOE/ER/60964-T1
ON: DE95006408; TRN: 95:002831
DOE Contract Number:  
FG02-90ER60964
Resource Type:
Technical Report
Resource Relation:
Other Information: PBD: 19 Dec 1994
Country of Publication:
United States
Language:
English
Subject:
56 BIOLOGY AND MEDICINE, APPLIED STUDIES; DNA ADDUCTS; DETECTION; HUMAN POPULATIONS; MUTATIONS; POLLUTANTS; GENETIC EFFECTS; PROGRESS REPORT; FLUORESCENCE; ELECTROPHORESIS; CHROMATOGRAPHY; TECHNOLOGY ASSESSMENT

Citation Formats

Giese, R W. New fluorescence methodology for detecting DNA adducts. Final progress report, May 1, 1991--November 30, 1994. United States: N. p., 1994. Web. doi:10.2172/29367.
Giese, R W. New fluorescence methodology for detecting DNA adducts. Final progress report, May 1, 1991--November 30, 1994. United States. doi:10.2172/29367.
Giese, R W. Mon . "New fluorescence methodology for detecting DNA adducts. Final progress report, May 1, 1991--November 30, 1994". United States. doi:10.2172/29367. https://www.osti.gov/servlets/purl/29367.
@article{osti_29367,
title = {New fluorescence methodology for detecting DNA adducts. Final progress report, May 1, 1991--November 30, 1994},
author = {Giese, R W},
abstractNote = {A new reagent, {open_quotes}BO-IMI{close_quotes}, has been developed that achieves, for the first time, single step, phosphate specific fluorescence labeling under aqueous conditions. Both 3{prime} and 5{prime} mononucleotides, including representative DNA adducts can be labeled. Included in this technique is a convenient procedure for postlabeling sample cleanup, leading to a practical detection of the products by capillary electrophoresis with laser fluorescence detection (CE-LIF). We consider that this new method will have a significant impact on the measurement of DNA adducts in human samples. This work was largely accomplished in the second half of our project. In the first half, we set up a new way to isolate DNA nucleotides from blood, worked with an initial, less specific technique for labeling DNA adducts, compared ionizing radiation vs oxidative damage to fluorescein labeled deoxyadenylic acid, and set up a capillary electrophoresis laser fluorescence detection system.},
doi = {10.2172/29367},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1994},
month = {12}
}