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Title: Cell damage in near-infrared multimode optical traps as a result of multiphoton absorption

Abstract

We report on cell damage of single cells confined in continuous-wave (cw), near-infrared (NIR) multimode optical traps as a result of multiphoton absorption phenomena. Trapping beams at NIR wavelengths less than 800nm are capable of damaging cells through a two-photon absorption process. Cell damage is more pronounced in multimode cw traps compared with single-frequency true cw NIR traps because of transient power enhancement by longitudinal mode beating. Partial mode locking in tunable cw Ti:sapphire lasers used as trapping beam sources can produce unstable subnanosecond pulses at certain wavelengths that amplify multiphoton absorption effects significantly. We recommend the use of single-frequency long-wavelength NIR trapping beams for optical micromanipulation of vital cells. {copyright} {ital 1996 Optical Society of America.}

Authors:
 [1]; ; ;  [2]
  1. Institute of Anatomy II, Friedrich Schiller University, D-07743 Jena (Germany)
  2. Beckman Laser Institute and Medical Clinic, University of California, Irvine, Irvine, California 92715 (United States)
Publication Date:
Sponsoring Org.:
USDOE
OSTI Identifier:
286585
DOE Contract Number:  
FG03-91ER61227
Resource Type:
Journal Article
Journal Name:
Optics Letters
Additional Journal Information:
Journal Volume: 21; Journal Issue: 14; Other Information: PBD: Jul 1996
Country of Publication:
United States
Language:
English
Subject:
55 BIOLOGY AND MEDICINE, BASIC STUDIES; ANIMAL CELLS; PHYSICAL RADIATION EFFECTS; TRAPPING; NEAR INFRARED RADIATION; DAMAGE; LASER RADIATION; MULTI-PHOTON PROCESSES

Citation Formats

Koenig, K, Liang, H, Berns, M W, and Tromberg, B J. Cell damage in near-infrared multimode optical traps as a result of multiphoton absorption. United States: N. p., 1996. Web. doi:10.1364/OL.21.001090.
Koenig, K, Liang, H, Berns, M W, & Tromberg, B J. Cell damage in near-infrared multimode optical traps as a result of multiphoton absorption. United States. https://doi.org/10.1364/OL.21.001090
Koenig, K, Liang, H, Berns, M W, and Tromberg, B J. 1996. "Cell damage in near-infrared multimode optical traps as a result of multiphoton absorption". United States. https://doi.org/10.1364/OL.21.001090.
@article{osti_286585,
title = {Cell damage in near-infrared multimode optical traps as a result of multiphoton absorption},
author = {Koenig, K and Liang, H and Berns, M W and Tromberg, B J},
abstractNote = {We report on cell damage of single cells confined in continuous-wave (cw), near-infrared (NIR) multimode optical traps as a result of multiphoton absorption phenomena. Trapping beams at NIR wavelengths less than 800nm are capable of damaging cells through a two-photon absorption process. Cell damage is more pronounced in multimode cw traps compared with single-frequency true cw NIR traps because of transient power enhancement by longitudinal mode beating. Partial mode locking in tunable cw Ti:sapphire lasers used as trapping beam sources can produce unstable subnanosecond pulses at certain wavelengths that amplify multiphoton absorption effects significantly. We recommend the use of single-frequency long-wavelength NIR trapping beams for optical micromanipulation of vital cells. {copyright} {ital 1996 Optical Society of America.}},
doi = {10.1364/OL.21.001090},
url = {https://www.osti.gov/biblio/286585}, journal = {Optics Letters},
number = 14,
volume = 21,
place = {United States},
year = {Mon Jul 01 00:00:00 EDT 1996},
month = {Mon Jul 01 00:00:00 EDT 1996}
}