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Title: Detection of small molecules by an evanescent wave fiber optic biosensor

Abstract

Detection of small molecules is important in environmental analyses as most pollutants are low molecular weight compounds. Utilizing the specificity of antibodies, the rapid signal transduction of optical fibers and the signal-to-noise discrimination of fluorescence, an evanescent wave fiber optic-based biosensor was modified for the detection of these molecules. To facilitate the isolation of personnel and equipment from hazardous environments, long partially clad optical fibers are employed. Antibodies were immobilized on the 10 cm sensing region at the distal portion of the optical fiber probe. A 200 {micro}l sample chamber was fabricated from a capillary tube. The core of the fiber in the sensing region is tapered for maximum signal recovery. A competitive immunoassay was performed. Basically, the small molecule competes with a fluorescently-labelled analog for the capture antibody binding sites. As the level of analyte increases in the sample, the fluorescent signal decreases. Two assays for the small molecules, trinitrotoluene (TNT) and polychlorinated biphenyl (PCB) will be described. Detection levels of 10 ng/ml TNT (8 ppb) have been achieved with this sensor.

Authors:
;  [1]
  1. Naval Research Lab., Washington, DC (United States). Center for Bio/Molecular Science and Engineering
Publication Date:
OSTI Identifier:
276368
Report Number(s):
CONF-950209-
TRN: IM9636%%152
Resource Type:
Conference
Resource Relation:
Conference: 4. international symposium on field screening methods for hazardous wastes and toxic chemicals, Las Vegas, NV (United States), 22-24 Feb 1995; Other Information: PBD: 1995; Related Information: Is Part Of Field screening methods for hazardous wastes and toxic chemicals. VIP-47, Volume 2; PB: 701 p.
Country of Publication:
United States
Language:
English
Subject:
54 ENVIRONMENTAL SCIENCES; 45 MILITARY TECHNOLOGY, WEAPONRY, AND NATIONAL DEFENSE; TNT; IMMUNOASSAY; POLYCHLORINATED BIPHENYLS; PERFORMANCE; SITE CHARACTERIZATION; SIGNAL-TO-NOISE RATIO

Citation Formats

Shriver-Lake, L C, and Ligler, F S. Detection of small molecules by an evanescent wave fiber optic biosensor. United States: N. p., 1995. Web.
Shriver-Lake, L C, & Ligler, F S. Detection of small molecules by an evanescent wave fiber optic biosensor. United States.
Shriver-Lake, L C, and Ligler, F S. Sun . "Detection of small molecules by an evanescent wave fiber optic biosensor". United States.
@article{osti_276368,
title = {Detection of small molecules by an evanescent wave fiber optic biosensor},
author = {Shriver-Lake, L C and Ligler, F S},
abstractNote = {Detection of small molecules is important in environmental analyses as most pollutants are low molecular weight compounds. Utilizing the specificity of antibodies, the rapid signal transduction of optical fibers and the signal-to-noise discrimination of fluorescence, an evanescent wave fiber optic-based biosensor was modified for the detection of these molecules. To facilitate the isolation of personnel and equipment from hazardous environments, long partially clad optical fibers are employed. Antibodies were immobilized on the 10 cm sensing region at the distal portion of the optical fiber probe. A 200 {micro}l sample chamber was fabricated from a capillary tube. The core of the fiber in the sensing region is tapered for maximum signal recovery. A competitive immunoassay was performed. Basically, the small molecule competes with a fluorescently-labelled analog for the capture antibody binding sites. As the level of analyte increases in the sample, the fluorescent signal decreases. Two assays for the small molecules, trinitrotoluene (TNT) and polychlorinated biphenyl (PCB) will be described. Detection levels of 10 ng/ml TNT (8 ppb) have been achieved with this sensor.},
doi = {},
url = {https://www.osti.gov/biblio/276368}, journal = {},
number = ,
volume = ,
place = {United States},
year = {1995},
month = {12}
}

Conference:
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