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Title: Phytotoxicity, uptake and metabolism of 1,4-dichlorobenzene by plant cells

Abstract

Phytotoxicity, uptake, and metabolism of 1,4-dichlorobenzene (1,4-DCB) by carrot (Daucus carota L.), soybean (Glycine max. L.), tomato (Lycopersicon esculentum Mill.), and red goosefoot (Chenopodiun rubrum L.) cell suspension cultures were studied. Sealed glass systems were utilized for the investigation because 1,4-DCB is volatile. The sealed systems affect the growth of plant cells, but do not provide different results when testing xenobiotic uptake and metabolism. 1,4-Dichlorobenzene (40 {micro}g in 40 ml medium) was taken up by carrot (49%), soybean (50%), and red goosefoot (62%) cells. Only the soybean cell cultures provided evidence of the existence of metabolites of this compound, probably conjugates of chlorophenols. Conditions for phytotoxicity tests were modified because the growth of cell cultures was affected when sealed for longer than 2 d. 1,4-Dichlorobenzene is toxic to cell cultures of the three tested plant species (tomato, soybean, and carrot). Concentrations of 0.5 mM caused 50% growth inhibition in carrot and soybean cultures. The tomato cultures were more sensitive, with 0.05 mM causing 50% growth inhibition.

Authors:
 [1]; ; ;  [2];  [1]
  1. Lancaster Univ. (United Kingdom). Inst. of Environmental and Biological Sciences
  2. Federal Agricultural Research Center, Braunschweig (Germany). Inst. of Plant Nutrition and Soil Science
Publication Date:
Sponsoring Org.:
USDOE
OSTI Identifier:
268015
Resource Type:
Journal Article
Journal Name:
Environmental Toxicology and Chemistry
Additional Journal Information:
Journal Volume: 15; Journal Issue: 7; Other Information: PBD: Jul 1996
Country of Publication:
United States
Language:
English
Subject:
56 BIOLOGY AND MEDICINE, APPLIED STUDIES; CHLORINATED AROMATIC HYDROCARBONS; TOXICITY; PLANTS; SENSITIVITY; ROOT ABSORPTION; TOMATOES; GLYCINE HISPIDA; CARROTS; GENETIC VARIABILITY; PLANT GROWTH

Citation Formats

Wang, M J, Chinese Academy of Sciences, Beijing, Bokern, M, Boehme, C, Harms, H, and Jones, K C. Phytotoxicity, uptake and metabolism of 1,4-dichlorobenzene by plant cells. United States: N. p., 1996. Web. doi:10.1897/1551-5028(1996)015<1109:PUAMOD>2.3.CO;2.
Wang, M J, Chinese Academy of Sciences, Beijing, Bokern, M, Boehme, C, Harms, H, & Jones, K C. Phytotoxicity, uptake and metabolism of 1,4-dichlorobenzene by plant cells. United States. https://doi.org/10.1897/1551-5028(1996)015<1109:PUAMOD>2.3.CO;2
Wang, M J, Chinese Academy of Sciences, Beijing, Bokern, M, Boehme, C, Harms, H, and Jones, K C. 1996. "Phytotoxicity, uptake and metabolism of 1,4-dichlorobenzene by plant cells". United States. https://doi.org/10.1897/1551-5028(1996)015<1109:PUAMOD>2.3.CO;2.
@article{osti_268015,
title = {Phytotoxicity, uptake and metabolism of 1,4-dichlorobenzene by plant cells},
author = {Wang, M J and Chinese Academy of Sciences, Beijing and Bokern, M and Boehme, C and Harms, H and Jones, K C},
abstractNote = {Phytotoxicity, uptake, and metabolism of 1,4-dichlorobenzene (1,4-DCB) by carrot (Daucus carota L.), soybean (Glycine max. L.), tomato (Lycopersicon esculentum Mill.), and red goosefoot (Chenopodiun rubrum L.) cell suspension cultures were studied. Sealed glass systems were utilized for the investigation because 1,4-DCB is volatile. The sealed systems affect the growth of plant cells, but do not provide different results when testing xenobiotic uptake and metabolism. 1,4-Dichlorobenzene (40 {micro}g in 40 ml medium) was taken up by carrot (49%), soybean (50%), and red goosefoot (62%) cells. Only the soybean cell cultures provided evidence of the existence of metabolites of this compound, probably conjugates of chlorophenols. Conditions for phytotoxicity tests were modified because the growth of cell cultures was affected when sealed for longer than 2 d. 1,4-Dichlorobenzene is toxic to cell cultures of the three tested plant species (tomato, soybean, and carrot). Concentrations of 0.5 mM caused 50% growth inhibition in carrot and soybean cultures. The tomato cultures were more sensitive, with 0.05 mM causing 50% growth inhibition.},
doi = {10.1897/1551-5028(1996)015<1109:PUAMOD>2.3.CO;2},
url = {https://www.osti.gov/biblio/268015}, journal = {Environmental Toxicology and Chemistry},
number = 7,
volume = 15,
place = {United States},
year = {Mon Jul 01 00:00:00 EDT 1996},
month = {Mon Jul 01 00:00:00 EDT 1996}
}