Characterization of the promoter region of the gene for the rat neutral and basic amino acid transporter and chromosomal localization of the human gene

Journal Article · Tue Aug 02 00:00:00 EDT 1994 · Proceedings of the National Academy of Sciences of the United States of America

DOI:https://doi.org/10.1073/pnas.91.16.7548· OSTI ID:255156

Yan, N; Mosckovitz, R; Gerber, L D ^[1]

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1. Roche Research Center, Nutley, NJ (United States); and others

The promoter region of the rat kidney neutral and basic amino acid transporter (NBAT) gene has been isolated and sequenced. The major transcription initiation site was mapped by primer extension. The entire promoter region and a set of 5{prime} deletions within it were expressed at a high level in LLC-PK1 cells using the luciferase indicator gene. Positive and negative regulatory elements in the promoter region were observed. A human genomic clone of the transporter was also obtained and was used to localize the NBAT gene at the p21 region of chromosome 2. 28 refs., 4 figs.

• Sponsoring Organization: USDOE
• OSTI ID: 255156
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America, Vol. 91, Issue 16; Other Information: PBD: 2 Aug 1994
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
PORINS
GENES
DNA SEQUENCING
HUMAN CHROMOSOME 2
GENETIC MAPPING
DNA-CLONING