Localization of the adenosine A2b receptor subtype (ADORA2B) to chromosome 17p11.2-p12 by FISH and PCR screening of somatic cell hybrids
- Garvan Institute for Medical Research, New South Wales (Australia)
- Women`s and Children`s Hospital, Adelaide (Australia); and others
Adenosine receptors have been implicated as important mediators of a diversity of physiological processes throughout the body. These receptors are members of the G protein-coupled receptor superfamily, a class of cell-surface receptors that, when activated, couple to a heterotrimeric G protein complex to effect signal transduction. Four different subtypes of adenosine receptor have been identified through molecular cloning and subsequent pharmacological and biochemical analyses. Of these subtypes, the Al and A2a receptors have been mapped to chromosome 22q11.2-q13.1 and 11q11-q13, respectively. The A3 receptor has been localized to chromosome 3 in the mouse, by interspecific backcross analysis, suggesting a human chromosomal localization of 1p13 from known mouse human linkage homologies. In determining the chromosomal localization of the A2a receptor, a minor hybridization peak was detected on chromosome 10q25.3-q26.3, and the authors of this study concluded that this site was likely to correspond to an adenosine A2-like receptor. With the molecular identification of the adenosine A2b receptor, the hybridization site on chromosome 10 was assigned to the A2b receptor (MIM 102777). We have used fluorescence in situ hybridization (FISH) and PCR screening of a somatic cell hybrid panel to determine the true chromosomal localization of the A2b adenosine receptor subtype. 12 refs., 1 fig., 1 tab.
- OSTI ID:
- 250185
- Journal Information:
- Genomics, Vol. 25, Issue 2; Other Information: PBD: 20 Jan 1995
- Country of Publication:
- United States
- Language:
- English
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