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Title: Polymorphisms and linkage analysis for ICAM-1 and the selectin gene cluster

Abstract

Genetic polymorphisms in leukocyte and endothelial cell adhesion molecules may be important variables with regard to susceptibility to multifactorial disease processes that include an inflammatory component. For this reason, polymorphisms were sought for intercellular adhesion molecule-1 (ICAM-1; gene symbol ICAM1) and for the three genes in the selectin cluster, P-selectin, L-selectin, and E-selectin (gene symbols SELP, SELL, and SELE, respectively). Two amino acid polymorphisms were identified for ICAM-1; Gly or Arg at codon 241 and Lys or Glu at codon 469. Dinucleotide repeat polymorphisms were identified in the 3{prime}-untranslated region for ICAM-1 and in intron 9 for P-selectin. Restriction fragment length polymorphisms were found using cDNAs for each of the three selectin genes as probes; E-selectin with BglII, P-selectin with ScaI, and L-selectin with HincII. Linkage analysis was performed for the selectin gene cluster and for ICAM-1 using the CEPH families; ICAM-1 is very tightly linked to the LDL receptor on chromosome 19, and the selectin cluster is linked to markers at chromosome 1q23. 41 refs., 2 tabs.

Authors:
; ;  [1]
  1. Baylor College of Medicine, Houston, TX (United States) [and others
Publication Date:
OSTI Identifier:
250043
Resource Type:
Journal Article
Resource Relation:
Journal Name: Genomics; Journal Volume: 21; Journal Issue: 3; Other Information: PBD: Jun 1994
Country of Publication:
United States
Language:
English
Subject:
55 BIOLOGY AND MEDICINE, BASIC STUDIES; GENES; RFLPS; MEMBRANE PROTEINS; HUMAN CHROMOSOME 9; GENETIC MAPPING; HUMAN CHROMOSOME 1; GENE MUTATIONS

Citation Formats

Vora, D.K., Rosenbloom, C.L., and Cottingham, R.W.. Polymorphisms and linkage analysis for ICAM-1 and the selectin gene cluster. United States: N. p., 1994. Web. doi:10.1006/geno.1994.1303.
Vora, D.K., Rosenbloom, C.L., & Cottingham, R.W.. Polymorphisms and linkage analysis for ICAM-1 and the selectin gene cluster. United States. doi:10.1006/geno.1994.1303.
Vora, D.K., Rosenbloom, C.L., and Cottingham, R.W.. Wed . "Polymorphisms and linkage analysis for ICAM-1 and the selectin gene cluster". United States. doi:10.1006/geno.1994.1303.
@article{osti_250043,
title = {Polymorphisms and linkage analysis for ICAM-1 and the selectin gene cluster},
author = {Vora, D.K. and Rosenbloom, C.L. and Cottingham, R.W.},
abstractNote = {Genetic polymorphisms in leukocyte and endothelial cell adhesion molecules may be important variables with regard to susceptibility to multifactorial disease processes that include an inflammatory component. For this reason, polymorphisms were sought for intercellular adhesion molecule-1 (ICAM-1; gene symbol ICAM1) and for the three genes in the selectin cluster, P-selectin, L-selectin, and E-selectin (gene symbols SELP, SELL, and SELE, respectively). Two amino acid polymorphisms were identified for ICAM-1; Gly or Arg at codon 241 and Lys or Glu at codon 469. Dinucleotide repeat polymorphisms were identified in the 3{prime}-untranslated region for ICAM-1 and in intron 9 for P-selectin. Restriction fragment length polymorphisms were found using cDNAs for each of the three selectin genes as probes; E-selectin with BglII, P-selectin with ScaI, and L-selectin with HincII. Linkage analysis was performed for the selectin gene cluster and for ICAM-1 using the CEPH families; ICAM-1 is very tightly linked to the LDL receptor on chromosome 19, and the selectin cluster is linked to markers at chromosome 1q23. 41 refs., 2 tabs.},
doi = {10.1006/geno.1994.1303},
journal = {Genomics},
number = 3,
volume = 21,
place = {United States},
year = {Wed Jun 01 00:00:00 EDT 1994},
month = {Wed Jun 01 00:00:00 EDT 1994}
}