Selection of chromosome 22-specific clones from human genomic BAC library using a chromosome-specific cosmid library pool
- Lawrence Livermore National Lab., CA (United States); and others
A new approach to rapidly identify chromosome-specific subsets of clones from a total human genomic library is described. The authors report here the results of screening a human bacterial artificial chromosome (BAC) library using the total pool of clones from a chromosome 22-specific cosmid library as a composite probe. The human BAC library was gridded on filters at high density and hybridized with DNA from the pooled chromosome 22-specific Lawrist library under suppressive conditions. In a single hybridization, they picked 280 candidates from the BAC library representing over 30,000 clones (or 1.2 x coverage of human genome). This subset contained more than 60% of the chromosome 22-specific BAC clones that were previously found to be present in the original BAC library. In principle, this approach can be applied to select a subset of clones from other global libraries with relatively large inserts using a pool from a regional library as a composite probe. It is important to note that the target and probe libraries must be based on vectors that share no homology with each other. 8 refs., 2 figs., 2 tabs.
- OSTI ID:
- 249990
- Journal Information:
- Genomics, Vol. 22, Issue 2; Other Information: PBD: 15 Jul 1994
- Country of Publication:
- United States
- Language:
- English
Similar Records
Cloning chromosome specific genes by reciprocal probing of arrayed cDNA and cosmid libraries
Multicolor chromosome banding (MCB) with YAC/BAC-based probes and region-specific microdissection DNA libraries