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Large-Scale Determination of Sequence, Structure, and Function Relationships in Cytosolic Glutathione Transferases across the Biosphere
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The First One-Pot Synthesis of l-7-Iodotryptophan from 7-Iodoindole and Serine, and an Improved Synthesis of Other l-7-Halotryptophans
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Structures and kinetics of Thermotoga maritima MetY reveal new insights into the predominant sulfurylation enzyme of bacterial methionine biosynthesis
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Combining Aldolases and Transaminases for the Synthesis of 2-Amino-4-hydroxybutanoic Acid
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Tryptophan Synthase Uses an Atypical Mechanism To Achieve Substrate Specificity
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The enzymology of cystathionine biosynthesis: strategies for the control of substrate and reaction specificity
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Biocatalytic synthesis of non-standard amino acids by a decarboxylative aldol reaction
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Biocatalytic One-Pot Synthesis of l -Tyrosine Derivatives from Monosubstituted Benzenes, Pyruvate, and Ammonia
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A synthesis of [1-(N-methyl-hemi-L-cystine)]-oxytocin and a study of its reaction with acetone
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Synthesis of β-Branched Tryptophan Analogues Using an Engineered Subunit of Tryptophan Synthase
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In Vivo Analysis of Various Substrates Utilized by Cystathionine -Synthase and O-Acetylhomoserine Sulfhydrylase in Methionine Biosynthesis
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Tryptophan Synthase: Biocatalyst Extraordinaire
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Unlocking Reactivity of TrpB: A General Biocatalytic Platform for Synthesis of Tryptophan Analogues
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Exploration of structure–function relationships in Escherichia coli cystathionine γ-synthase and cystathionine β-lyase via chimeric constructs and site-specific substitutions
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June 2013 |
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Directed evolution of the tryptophan synthase β-subunit for stand-alone function recapitulates allosteric activation
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Engineering enzymes for noncanonical amino acid synthesis
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January 2018 |
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Substrate multiplexed protein engineering facilitates promiscuous biocatalytic synthesis
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Biosynthesis of Mycotoxin Fusaric Acid and Application of a PLP-Dependent Enzyme for Chemoenzymatic Synthesis of Substituted l-Pipecolic Acids
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November 2020 |
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Engineered L‐Serine Hydroxymethyltransferase from Streptococcus thermophilus for the Synthesis of α,α‐Dialkyl‐α‐Amino Acids
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Expanding and Reprogramming the Genetic Code of Cells and Animals
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Chemoenzymatic synthesis and in situ application of S-adenosyl-l-methionine analogs
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Site-Selective C–H Halogenation Using Flavin-Dependent Halogenases Identified via Family-Wide Activity Profiling
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Functional Genomics: Expression Analysis ofEscherichia coli Growing on Minimal and Rich Media
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Cloning and overexpression of the oah1 gene encoding O-acetyl-l-homoserine sulfhydrylase of Thermus thermophilus HB8 and characterization of the gene product
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Engineering Enzyme Substrate Scope Complementarity for Promiscuous Cascade Synthesis of 1,2‐Amino Alcohols
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Unusual Amino Acids in Medicinal Chemistry
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Interconversion of a pair of active-site residues inEscherichia colicystathionine γ-synthase,E. colicystathionine β-lyase, andSaccharomyces cerevisiaecystathionine γ-lyase and development of tools for the investigation of their mechanisms and reaction specificity
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Canavanine utilization via homoserine and hydroxyguanidine by a PLP-dependent γ-lyase in Pseudomonadaceae and Rhizobiales
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Scalable continuous evolution for the generation of diverse enzyme variants encompassing promiscuous activities
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Beyond 20 in the 21st Century: Prospects and Challenges of Non-canonical Amino Acids in Peptide Drug Discovery
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Cutting Long Syntheses Short: Access to Non-Natural Tyrosine Derivatives Employing an Engineered Tyrosine Phenol Lyase
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Characterization of Homocysteine γ-Lyase from Submerged and Solid Cultures of Aspergillus fumigatus ASH (JX006238)
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Methionine gamma-lyase: The unique reaction mechanism, physiological roles, and therapeutic applications against infectious diseases and cancers
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Enzymatic Synthesis of β-Hydroxy-α-amino Acids Based on Recombinant d - and l -Threonine Aldolases
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PLP-dependent enzymes as important biocatalysts for the pharmaceutical, chemical and food industries: a structural and mechanistic perspective
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Genome mining for unknown–unknown natural products
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January 2023 |
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Engineered Biosynthesis of β-Alkyl Tryptophan Analogues
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October 2018 |
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HAMA: a multiplexed LC-MS/MS assay for specificity profiling of adenylate-forming enzymes
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January 2019 |
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A Panel of TrpB Biocatalysts Derived from Tryptophan Synthase through the Transfer of Mutations that Mimic Allosteric Activation
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Appendix. Purification, molecular weight, and NH2-terminal sequence of cystathionine gamma-synthase of Escherichia coli.
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Stereoselective Synthesis of β‐Branched Aromatic α‐Amino Acids by Biocatalytic Dynamic Kinetic Resolution**
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Reaction mechanism of Escherichia coli cystathionine .gamma.-synthase: direct evidence for a pyridoxamine derivative of vinylgloxylate as a key intermediate in pyridoxal phosphate dependent .gamma.-elimination and .gamma.-replacement reactions
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On the Sequence of Bond Formation in Loline Alkaloid Biosynthesis
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Expanding the Genetic code
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A convenient enzymatic synthesis of l-halotryptophans
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Scalable and Selective β‐Hydroxy‐α‐Amino Acid Synthesis Catalyzed by Promiscuous l‐Threonine Transaldolase ObiH
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November 2021 |
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Investigation of β‐Substitution Activity of O‐Acetylserine Sulfhydrolase from Citrullus vulgaris
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Efficient Chemoenzymatic Synthesis of α-Aryl Aldehydes as Intermediates in C–C Bond Forming Biocatalytic Cascades
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The Methionine-Repressible Homoserine Dehydrogenase and Aspartokinase Activities of Escherichia coli K12. Preparation of the Homogeneous Protein Catalyzing the Two Activities. Molecular Weight of the Native Enzyme and of its Subunits
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Biocatalytic Asymmetric Synthesis of Chiral Amines from Ketones Applied to Sitagliptin Manufacture
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Sonogashira diversification of unprotected halotryptophans, halotryptophan containing tripeptides; and generation of a new to nature bromo-natural product and its diversification in water
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Enzyme function prediction using contrastive learning
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Homocysteine Synthesis in Yeast: Partial Purification and Properties of O-Acetylhomoserine Sulfhydrylase*
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Synthesis of γ-Hydroxy-α-amino Acid Derivatives by Enzymatic Tandem Aldol Addition–Transamination Reactions
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Panoramic view of a superfamily of phosphatases through substrate profiling
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Site-Selective Deuteration of Amino Acids through Dual-Protein Catalysis
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Reprogramming Nonribosomal Peptide Synthetases for “Clickable” Amino Acids
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Asymmetric Alkylation of Ketones Catalyzed by Engineered TrpB
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Enzyme Activity Fingerprinting with Substrate Cocktails
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