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Native Top-Down Mass Spectrometry with Collisionally Activated Dissociation Yields Higher-Order Structure Information for Protein Complexes

Journal Article · · Journal of the American Chemical Society
DOI:https://doi.org/10.1021/jacs.2c06726· OSTI ID:2422518
 [1];  [2];  [2];  [2];  [2];  [2];  [3];  [4];  [5]
  1. Department of Chemistry and Biochemistry, University of California−Los Angeles, Los Angeles, California 90095, United States; OSTI
  2. Department of Chemistry and Biochemistry, University of California−Los Angeles, Los Angeles, California 90095, United States
  3. Molecular Biology Institute, University of California−Los Angeles, Los Angeles, California 90095, United States
  4. Department of Chemistry and Biochemistry, University of California−Los Angeles, Los Angeles, California 90095, United States; UCLA-DOE Institute, University of California−Los Angeles, Los Angeles, California 90095, United States; Molecular Biology Institute, University of California−Los Angeles, Los Angeles, California 90095, United States
  5. Department of Chemistry and Biochemistry, University of California−Los Angeles, Los Angeles, California 90095, United States; Department of Biological Chemistry, University of California−Los Angeles, Los Angeles, California 90095, United States; UCLA-DOE Institute, University of California−Los Angeles, Los Angeles, California 90095, United States; Molecular Biology Institute, University of California−Los Angeles, Los Angeles, California 90095, United States
+ Show Author Affiliations
Not provided.
Research Organization:
Univ. of California, Los Angeles, CA (United States)
Sponsoring Organization:
USDOE Office of Science (SC)
DOE Contract Number:
FC02-02ER63421
OSTI ID:
2422518
Journal Information:
Journal of the American Chemical Society, Journal Name: Journal of the American Chemical Society Journal Issue: 48 Vol. 144; ISSN 0002-7863
Publisher:
American Chemical Society (ACS)
Country of Publication:
United States
Language:
English

References (31)

Salt Bridge Rearrangement (SaBRe) Explains the Dissociation Behavior of Noncovalent Complexes journal April 2016
Fourier Transform-Ion Cyclotron Resonance Mass Spectrometry as a Platform for Characterizing Multimeric Membrane Protein Complexes journal October 2017
Mass spectrometry of intact membrane protein complexes journal March 2013
Towards understanding the formation of internal fragments generated by collisionally activated dissociation for top-down mass spectrometry journal February 2022
Electron transfer dissociation provides higher-order structural information of native and partially unfolded protein complexes journal July 2015
Increasing Top-Down Mass Spectrometry Sequence Coverage by an Order of Magnitude through Optimized Internal Fragment Generation and Assignment journal April 2021
Structural Characterization of Dihydrofolate Reductase Complexes by Top-Down Ultraviolet Photodissociation Mass Spectrometry journal July 2015
Native Top-Down Mass Spectrometry and Ion Mobility Spectrometry of the Interaction of Tau Protein with a Molecular Tweezer Assembly Modulator journal July 2018
Internal Fragments Generated from Different Top-Down Mass Spectrometry Fragmentation Methods Extend Protein Sequence Coverage journal June 2021
ClipsMS: An Algorithm for Analyzing Internal Fragments Resulting from Top-Down Mass Spectrometry journal March 2021
Native Top-Down Electrospray Ionization-Mass Spectrometry of 158 kDa Protein Complex by High-Resolution Fourier Transform Ion Cyclotron Resonance Mass Spectrometry journal December 2013
Native Electrospray and Electron-Capture Dissociation in FTICR Mass Spectrometry Provide Top-Down Sequencing of a Protein Component in an Intact Protein Assembly journal December 2010
Top-Down ESI-ECD-FT-ICR Mass Spectrometry Localizes Noncovalent Protein-Ligand Binding Sites journal November 2006
Native Mass Spectrometry Imaging of Proteins and Protein Complexes by Nano-DESI journal March 2021
Elucidating the site of protein-ATP binding by top-down mass spectrometry journal June 2010
Higher-energy C-trap dissociation for peptide modification analysis journal August 2007
High Mass Analysis with a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer: From Inorganic Salt Clusters to Antibody Conjugates and Beyond journal March 2020
Alternate Dissociation Pathways Identified in Charge-Reduced Protein Complex Ions journal June 2010
Characterization of Native Protein Complexes Using Ultraviolet Photodissociation Mass Spectrometry journal September 2014
Higher-order structural characterisation of native proteins and complexes by top-down mass spectrometry journal January 2020
Revealing Ligand Binding Sites and Quantifying Subunit Variants of Noncovalent Protein Complexes in a Single Native Top-Down FTICR MS Experiment journal June 2014
An integrated native mass spectrometry and top-down proteomics method that connects sequence to structure and function of macromolecular complexes journal January 2018
Top or Middle? Up or Down? Toward a Standard Lexicon for Protein Top-Down and Allied Mass Spectrometry Approaches journal May 2019
Dissecting the Dynamic Conformations of the Metamorphic Protein Lymphotactin journal October 2014
Top-down characterization of endogenous protein complexes with native proteomics journal November 2017
Top-Down Characterization of Denatured Proteins and Native Protein Complexes Using Electron Capture Dissociation Implemented within a Modified Ion Mobility-Mass Spectrometer journal January 2020
Structural characterization of holo- and apo-myoglobin in the gas phase by ultraviolet photodissociation mass spectrometry journal January 2015
Charge Movement and Structural Changes in the Gas-Phase Unfolding of Multimeric Protein Complexes Captured by Native Top-Down Mass Spectrometry journal December 2019
Comparative Structural Analysis of 20S Proteasome Ortholog Protein Complexes by Native Mass Spectrometry journal April 2020
Internal Fragments Generated by Electron Ionization Dissociation Enhance Protein Top-Down Mass Spectrometry journal August 2020
Analyzing Internal Fragmentation of Electrosprayed Ubiquitin Ions During Beam-Type Collisional Dissociation journal February 2015

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