Characterization of a Host-Specific Toxic Activity Produced by Bipolaris cookei , Causal Agent of Target Leaf Spot of Sorghum

Samira, Rozalynne; Lopez, Luis Fernando Samayoa; Holland, James; Balint-Kurti, Peter J.

doi:10.1094/phyto-11-22-0427-r

Characterization of a Host-Specific Toxic Activity Produced by Bipolaris cookei , Causal Agent of Target Leaf Spot of Sorghum

Journal Article · 01 July 2023 · Phytopathology

DOI:https://doi.org/10.1094/phyto-11-22-0427-r· OSTI ID:2420325

^[1]; Lopez, Luis Fernando Samayoa ^[2]; Holland, James ^[3]; ^[4]

Department of Entomology and Plant Pathology, North Carolina State University, Raleigh, NC 27695-7613; Department of Plant and Soil Science, Texas Tech University, Lubbock, TX 79409; OSTI
Department of Crop and Soil Sciences, North Carolina State University, Raleigh, NC 27695-7620
Department of Crop and Soil Sciences, North Carolina State University, Raleigh, NC 27695-7620; USDA-ARS Plant Science Research Unit, Raleigh, NC 27695
Department of Entomology and Plant Pathology, North Carolina State University, Raleigh, NC 27695-7613; USDA-ARS Plant Science Research Unit, Raleigh, NC 27695

Target leaf spot (TLS) of sorghum, caused by the necrotrophic fungus Bipolaris cookei, can cause severe yield loss in many parts of the world. We grew B. cookei in liquid culture and observed that the resulting culture filtrate (CF) was differentially toxic when infiltrated into the leaves of a population of 288 diverse sorghum lines. In this population, we found a significant correlation between high CF sensitivity and susceptibility to TLS. This suggests that the toxin produced in culture may play a role in the pathogenicity of B. cookei in the field. We demonstrated that the toxic activity is light sensitive and, surprisingly, insensitive to pronase, suggesting that it is not proteinaceous. We identified the two sorghum genetic loci most associated with the response to CF in this population. Screening seedlings with B. cookei CF could be a useful approach for prescreening germplasm for TLS resistance.

Research Organization:: North Carolina State University, Raleigh, NC (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

DOE Contract Number:: SC0014116

OSTI ID:: 2420325

Journal Information:: Phytopathology, Journal Name: Phytopathology Journal Issue: 7 Vol. 113; ISSN 0031-949X

Publisher:: American Phytopathological Society

Country of Publication:: United States

Language:: English

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