Transcript and metabolite network perturbations in lignin biosynthetic mutants of Arabidopsis

Wang, Peng; Guo, Longyun; Morgan, John; Dudareva, Natalia; Chapple, Clint

doi:10.1093/plphys/kiac344

Transcript and metabolite network perturbations in lignin biosynthetic mutants of Arabidopsis

Journal Article · 26 July 2022 · Plant Physiology (Bethesda)

DOI:https://doi.org/10.1093/plphys/kiac344· OSTI ID:2419821

^[1]; Guo, Longyun; ; ;

OSTI

Abstract

Lignin, one of the most abundant polymers in plants, is derived from the phenylpropanoid pathway, which also gives rise to an array of metabolites that are essential for plant fitness. Genetic engineering of lignification can cause drastic changes in transcription and metabolite accumulation with or without an accompanying development phenotype. To understand the impact of lignin perturbation, we analyzed transcriptome and metabolite data from the rapidly lignifying stem tissue in 13 selected phenylpropanoid mutants and wild-type Arabidopsis (Arabidopsis thaliana). Our dataset contains 20,974 expressed genes, of which over 26% had altered transcript levels in at least one mutant, and 18 targeted metabolites, all of which displayed altered accumulation in at least one mutant. We found that lignin biosynthesis and phenylalanine supply via the shikimate pathway are tightly co-regulated at the transcriptional level. The hierarchical clustering analysis of differentially expressed genes (DEGs) grouped the 13 mutants into 5 subgroups with similar profiles of mis-regulated genes. Functional analysis of the DEGs in these mutants and correlation between gene expression and metabolite accumulation revealed system-wide effects on transcripts involved in multiple biological processes.

Research Organization:: Purdue Univ., West Lafayette, IN (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

DOE Contract Number:: SC0008628

OSTI ID:: 2419821

Journal Information:: Plant Physiology (Bethesda), Journal Name: Plant Physiology (Bethesda) Journal Issue: 4 Vol. 190; ISSN 0032-0889

Publisher:: American Society of Plant Biologists

Country of Publication:: United States

Language:: English

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