Expanding the Scope of Bacterial CRISPR Activation with PAM-Flexible dCas9 Variants

Kiattisewee, Cholpisit; Karanjia, Ava V.; Legut, Mateusz; Daniloski, Zharko; Koplik, Samantha E.; Nelson, Joely; Kleinstiver, Benjamin P.; Sanjana, Neville E.; Carothers, James M.; Zalatan, Jesse G.

doi:10.1021/acssynbio.2c00405

Expanding the Scope of Bacterial CRISPR Activation with PAM-Flexible dCas9 Variants

Journal Article · 15 November 2022 · ACS Synthetic Biology

DOI:https://doi.org/10.1021/acssynbio.2c00405· OSTI ID:2418701

^[1]; ^[2]; Legut, Mateusz ^[3]; Daniloski, Zharko ^[3]; Koplik, Samantha E. ^[4]; Nelson, Joely ^[5]; ^[6]; Sanjana, Neville E. ^[3]; Carothers, James M. ^[7]; ^[8]

Molecular Engineering & Sciences Institute and Center for Synthetic Biology, University of Washington, Seattle, Washington98195, United States; OSTI
Molecular Engineering & Sciences Institute and Center for Synthetic Biology, University of Washington, Seattle, Washington98195, United States; Department of Chemical Engineering, University of Washington, Seattle, Washington98195, United States
New York Genome Center, New York, New York10013, United States; Department of Biology, New York University, New York, New York10013, United States
Department of Bioengineering, University of Washington, Seattle, Washington98195, United States
Molecular Engineering & Sciences Institute and Center for Synthetic Biology, University of Washington, Seattle, Washington98195, United States
Center for Genomic Medicine, Massachusetts General Hospital, Boston, Massachusetts02114, United States; Department of Pathology, Massachusetts General Hospital, Boston, Massachusetts02114, United States; Department of Pathology, Harvard Medical School, Boston, Massachusetts02115, United States
Molecular Engineering & Sciences Institute and Center for Synthetic Biology, University of Washington, Seattle, Washington98195, United States; Department of Chemical Engineering, University of Washington, Seattle, Washington98195, United States; Department of Bioengineering, University of Washington, Seattle, Washington98195, United States
Molecular Engineering & Sciences Institute and Center for Synthetic Biology, University of Washington, Seattle, Washington98195, United States; Department of Chemical Engineering, University of Washington, Seattle, Washington98195, United States; Department of Chemistry, University of Washington, Seattle, Washington98195, United States

Not provided.

Research Organization:: Univ. of Washington, Seattle, WA (United States)

Sponsoring Organization:: USDOE Office of Energy Efficiency and Renewable Energy (EERE)

DOE Contract Number:: EE0008927

OSTI ID:: 2418701

Journal Information:: ACS Synthetic Biology, Journal Name: ACS Synthetic Biology Journal Issue: 12 Vol. 11; ISSN 2161-5063

Publisher:: American Chemical Society (ACS)

Country of Publication:: United States

Language:: English

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