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Title: PCR detection of groundwater bacteria associated with colloidal transport

Abstract

Colloidal transport may increase the amount of contaminant material than that which could be transported by water flow alone. The role of colloids in groundwater contaminant transport is complicated and may involve many different processes, including sorption of elements onto colloidal particles, coagulation/dissolution, adsorption onto solid surfaces, filtration, and migration. Bacteria are known to concentrate minerals and influence the transport of compounds in aqueous environments and may also serve as organic colloids, thereby influencing subsurface transport of radionuclides and other contaminants. The initial phase of the project consisted of assembling a list of bacteria capable of sequestering or facilitating mineral transport. The development and optimization of the PCR amplification assay for the detection of the organisms of interest, and the examination of regional groundwaters for those organisms, are presented for subsequent research.

Authors:
; ;
Publication Date:
Research Org.:
Nevada Univ., Las Vegas, NV (United States). Harry Reid Center for Environmental Studies
Sponsoring Org.:
USDOE, Washington, DC (United States); USDOE Office of Civilian Radioactive Waste Management, Washington, DC (United States)
OSTI Identifier:
237359
Report Number(s):
DOE/NV/10872-T269
ON: DE96011086; BR: DB0102000/DB0102100; TRN: 96:014540
DOE Contract Number:
FC08-90NV10872
Resource Type:
Technical Report
Resource Relation:
Other Information: PBD: 29 Feb 1996
Country of Publication:
United States
Language:
English
Subject:
54 ENVIRONMENTAL SCIENCES; 05 NUCLEAR FUELS; GROUND WATER; RADIONUCLIDE MIGRATION; MONITORING; BACTERIA; COLLOIDS; BIOADSORBENTS; CONTAMINATION; DNA POLYMERASES

Citation Formats

Cruz-Perez, P., Stetzenbach, L.D., and Alvarez, A.J. PCR detection of groundwater bacteria associated with colloidal transport. United States: N. p., 1996. Web. doi:10.2172/237359.
Cruz-Perez, P., Stetzenbach, L.D., & Alvarez, A.J. PCR detection of groundwater bacteria associated with colloidal transport. United States. doi:10.2172/237359.
Cruz-Perez, P., Stetzenbach, L.D., and Alvarez, A.J. Thu . "PCR detection of groundwater bacteria associated with colloidal transport". United States. doi:10.2172/237359. https://www.osti.gov/servlets/purl/237359.
@article{osti_237359,
title = {PCR detection of groundwater bacteria associated with colloidal transport},
author = {Cruz-Perez, P. and Stetzenbach, L.D. and Alvarez, A.J.},
abstractNote = {Colloidal transport may increase the amount of contaminant material than that which could be transported by water flow alone. The role of colloids in groundwater contaminant transport is complicated and may involve many different processes, including sorption of elements onto colloidal particles, coagulation/dissolution, adsorption onto solid surfaces, filtration, and migration. Bacteria are known to concentrate minerals and influence the transport of compounds in aqueous environments and may also serve as organic colloids, thereby influencing subsurface transport of radionuclides and other contaminants. The initial phase of the project consisted of assembling a list of bacteria capable of sequestering or facilitating mineral transport. The development and optimization of the PCR amplification assay for the detection of the organisms of interest, and the examination of regional groundwaters for those organisms, are presented for subsequent research.},
doi = {10.2172/237359},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Thu Feb 29 00:00:00 EST 1996},
month = {Thu Feb 29 00:00:00 EST 1996}
}

Technical Report:

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