Long non-coding RNA XIST inhibited breast cancer cell growth, migration, and invasion via miR-155/CDX1 axis
- Department of Oncology, Dongguan People's Hospital, Southern Medical University, Dongguan (China)
- Clinical Research Center, Dongguan People's Hospital, Southern Medical University, Dongguan (China)
- Department of Galactophore, Dongguan People's Hospital, Southern Medical University, Dongguan (China)
- Department of Pathology, Dongguan People's Hospital, Southern Medical University, Dongguan (China)
- Department of Reproductive Medicine, The First Affiliated Hospital of Dali University, Dali, Yunnan (China)
Highlights: • LncRNA XIST was significantly down-regulated in breast cancer. • LncRNA XIST depressed the growth, migration and invasion of breast cancer. • MiR-155 was a direct target of XIST. • LncRNA XIST exerted its function via miR-155/CDX1 axis. Long non-coding RNA (lncRNA) is an important member of non-coding RNA family and emerging evidence has indicated that it plays a pivotal role in many physiological and pathological processes. The lncRNA X inactive specific transcript (XIST) is a potential tumour suppressor in some types of cancers. However, the expression and function of XIST in breast cancer remain largely unclear. The objective of this study was to evaluate the expression and biological role of XIST in breast cancer. The results showed that XIST was significantly down-regulated in breast cancer tissues and cell lines. Further functional analysis indicated that overexpression of XIST remarkably inhibited breast cancer cell growth, migration, and invasion. The results of luciferase reporter assays verified that miR-155 was a direct target of XIST in breast cancer. Moreover, caudal-type homeobox 1 (CDX1) was identified as a direct target of miR-155 and miR-155/CDX1 rescued the effects of XIST in breast cancer cells. Taken together, our results suggest that XIST is down-regulated in breast cancer and suppresses breast cancer cell growth, migration, and invasion via the miR-155/CDX1 axis.
- OSTI ID:
- 23137210
- Journal Information:
- Biochemical and Biophysical Research Communications, Vol. 498, Issue 4; Other Information: Copyright (c) 2018 Published by Elsevier Inc.; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
- Country of Publication:
- United States
- Language:
- English
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