A putative cyclin, SiPHO80 from root endophytic fungus Serendipita indica regulates phosphate homeostasis, salinity and heavy metal toxicity tolerance
- School of Life Sciences, Jawaharlal Nehru University, New Delhi, 110067 (India)
Highlights: • SiPHO80 of S. indica gets upregulated in high Phosphate condition. • SiPHO80 restores Pi homeostasis in S. cerevisiae PHO80 mutants. • SiPHO80 rescues S. cerevisiae PHO80 mutants in salinity stress and heavy metal toxicity. Serendipita indica previously known as Piriformospora indica is an arbuscular mycorrhizal fungi (AMF) like endophytic fungus which can be cultivated axenically and colonizes an array of plants, thereby promoting their growth and confers biotic and abiotic stress tolerance to the colonized host plant. It efficiently sequestrates heavy metals and defends host plant against heavy metal-induced toxicity. In the present study, SiPHO80, a homologue of S. cerevisiae PHO80 was isolated from S. indica and functionally characterized in S. cerevisiae. SiPHO80 has conserved ‘cyclin box’ domain and closely related to negative regulator cyclin of the wood decaying fungi. In S. indica, its expression gets upregulated in phosphate-rich media. The regulation of Pi homeostasis which was disrupted in S. cerevisiae Δpho80 grown under high Pi condition was restored upon complementation with SiPHO80. Also, the expression of SiPHO80 in Δpho80 mutant restored osmotolerance and heavy metal tolerance. This is the first report of a cyclin which is involved in Pi homeostasis, salt tolerance, heavy metal toxicity tolerance in any plant growth promoting endophytic fungi.
- OSTI ID:
- 23107879
- Journal Information:
- Biochemical and Biophysical Research Communications, Vol. 507, Issue 1-4; Other Information: Copyright (c) 2018 Elsevier Inc. All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
- Country of Publication:
- United States
- Language:
- English
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