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Title: A fiber optic fluoroimmunosensor for the detection of polychlorinated biphenyls

Abstract

There currently exists a critical and growing need for cost-effective, real-time, and in situ methods for detection of environmental pollutants such as polychlorinated biphenyls (PCBs). Herein reported is a evanescent wave fluoroimmunosensor for measurement of PCBS. 2,4,5-Trichlorophenoxybutyric acid (TCPB) was cross-linked to fluoresce in ethyleneamine (TCPB-FL) an used as an optical signal generator. Protein A purified IgG, obtained from anti-PCB serum, was immobilized on quartz fibers and used as the biological recognition element in an evanescent wave fluorimeter. The optical signal was generated by the binding of the fluorescent probe TCPB-FL to the antibody (Ab)-coated fiber. Fluorescent probe concentrations in the nM range resulted in reversible binding. Several Aroclors displaced the fluorescent probe (TCPB-FL) in a concentration-dependent manner. Aroclor 1248 was detected from the ppb to ppm range. Aroclor 1242 was also measured in methanol extracts from standard reference material (SRM) soils.

Authors:
;  [1]; ; ; ;  [2]
  1. Environmental Protection Agency, Las Vegas, NV (United States). Environmental Monitoring Systems Lab.
  2. UMAB, Baltimore, MD (United States)
Publication Date:
OSTI Identifier:
230954
Report Number(s):
CONF-950209-
TRN: IM9623%%264
Resource Type:
Conference
Resource Relation:
Conference: 4. international symposium on field screening methods for hazardous wastes and toxic chemicals, Las Vegas, NV (United States), 22-24 Feb 1995; Other Information: PBD: 1995; Related Information: Is Part Of Field screening methods for hazardous wastes and toxic chemicals. VIP-47, Volume 1; PB: 733 p.
Country of Publication:
United States
Language:
English
Subject:
54 ENVIRONMENTAL SCIENCES; POLYCHLORINATED BIPHENYLS; IMMUNOASSAY; TECHNOLOGY ASSESSMENT; FIBER OPTICS; DATA; FLUORESCENCE

Citation Formats

Rogers, K R, Van Emon, J M, Kline, R, Wright, J, Zhao, Q, and Eldefrawi, M E. A fiber optic fluoroimmunosensor for the detection of polychlorinated biphenyls. United States: N. p., 1995. Web.
Rogers, K R, Van Emon, J M, Kline, R, Wright, J, Zhao, Q, & Eldefrawi, M E. A fiber optic fluoroimmunosensor for the detection of polychlorinated biphenyls. United States.
Rogers, K R, Van Emon, J M, Kline, R, Wright, J, Zhao, Q, and Eldefrawi, M E. Sun . "A fiber optic fluoroimmunosensor for the detection of polychlorinated biphenyls". United States.
@article{osti_230954,
title = {A fiber optic fluoroimmunosensor for the detection of polychlorinated biphenyls},
author = {Rogers, K R and Van Emon, J M and Kline, R and Wright, J and Zhao, Q and Eldefrawi, M E},
abstractNote = {There currently exists a critical and growing need for cost-effective, real-time, and in situ methods for detection of environmental pollutants such as polychlorinated biphenyls (PCBs). Herein reported is a evanescent wave fluoroimmunosensor for measurement of PCBS. 2,4,5-Trichlorophenoxybutyric acid (TCPB) was cross-linked to fluoresce in ethyleneamine (TCPB-FL) an used as an optical signal generator. Protein A purified IgG, obtained from anti-PCB serum, was immobilized on quartz fibers and used as the biological recognition element in an evanescent wave fluorimeter. The optical signal was generated by the binding of the fluorescent probe TCPB-FL to the antibody (Ab)-coated fiber. Fluorescent probe concentrations in the nM range resulted in reversible binding. Several Aroclors displaced the fluorescent probe (TCPB-FL) in a concentration-dependent manner. Aroclor 1248 was detected from the ppb to ppm range. Aroclor 1242 was also measured in methanol extracts from standard reference material (SRM) soils.},
doi = {},
url = {https://www.osti.gov/biblio/230954}, journal = {},
number = ,
volume = ,
place = {United States},
year = {1995},
month = {12}
}

Conference:
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