skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Exosomes derived from imatinib-resistant chronic myeloid leukemia cells mediate a horizontal transfer of drug-resistant trait by delivering miR-365

Abstract

Highlights: • Imatinib resistant CML cells-derived exosomes are internalized into sensitive CML cells. • Imatinib resistant CML cells-derived exosomes confer drug resistance trait to sensitive CML cells. • Exosomes mediate horizontal transfer of miR-365 between imatinib resistant CML cells and sensitive ones. • MiR-365 lowers chemosensitivity and apoptosis in imatinib-sensitive CML cells. Chronic myeloid leukemia (CML) is a malignant disorder of hematopoietic stem/progenitor cells. Majority of patients can be effectively treated with tyrosine kinase inhibitors (TKIs) such as imatinib, but a portion of patients will develop drug resistance. Accumulated evidences have identified exosomes in cancer as promoters of tumor progression. Herein, we found that exosomes derived from imatinib resistant CML cells can be internalized into sensitive CML cells and confer drug-resistance traits. We also demonstrated a significant higher level of miR-365 in exosomes derived from drug-resistant CML cells compared with those from sensitive ones using microarray and qRT-PCR. The imatinib sensitive CML cells transfected with pre-miR-365 displayed lower chemosensitivity and apoptosis rate compared with controls. We further confirmed that exosomal transfer of miR-365 induced drug resistance by inhibiting expression of pro-apoptosis protein in sensitive CML cells. In conclusion, our study reveals that exosomes mediate a horizontal transfer of drug-resistant traitmore » in chronic myeloid leukemia cell by delivering miR-365.« less

Authors:
; ; ; ;  [1];
  1. Department of Clinical Laboratory, the Second Affiliated Hospital of Nanchang University, No.1 Min De Road, Nanchang (China)
Publication Date:
OSTI Identifier:
23082491
Resource Type:
Journal Article
Journal Name:
Experimental Cell Research
Additional Journal Information:
Journal Volume: 362; Journal Issue: 2; Other Information: Copyright (c) 2017 Elsevier Inc. All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0014-4827
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; APOPTOSIS; DRUGS; MYELOID LEUKEMIA; PHOSPHOTRANSFERASES; POLYMERASE CHAIN REACTION; TYROSINE

Citation Formats

Min, Qing-Hua, Wang, Xiao-Zhong, Zhang, Jing, Chen, Qing-Gen, Li, Shu-Qi, and others, and. Exosomes derived from imatinib-resistant chronic myeloid leukemia cells mediate a horizontal transfer of drug-resistant trait by delivering miR-365. United States: N. p., 2018. Web. doi:10.1016/J.YEXCR.2017.12.001.
Min, Qing-Hua, Wang, Xiao-Zhong, Zhang, Jing, Chen, Qing-Gen, Li, Shu-Qi, & others, and. Exosomes derived from imatinib-resistant chronic myeloid leukemia cells mediate a horizontal transfer of drug-resistant trait by delivering miR-365. United States. https://doi.org/10.1016/J.YEXCR.2017.12.001
Min, Qing-Hua, Wang, Xiao-Zhong, Zhang, Jing, Chen, Qing-Gen, Li, Shu-Qi, and others, and. 2018. "Exosomes derived from imatinib-resistant chronic myeloid leukemia cells mediate a horizontal transfer of drug-resistant trait by delivering miR-365". United States. https://doi.org/10.1016/J.YEXCR.2017.12.001.
@article{osti_23082491,
title = {Exosomes derived from imatinib-resistant chronic myeloid leukemia cells mediate a horizontal transfer of drug-resistant trait by delivering miR-365},
author = {Min, Qing-Hua and Wang, Xiao-Zhong and Zhang, Jing and Chen, Qing-Gen and Li, Shu-Qi and others, and},
abstractNote = {Highlights: • Imatinib resistant CML cells-derived exosomes are internalized into sensitive CML cells. • Imatinib resistant CML cells-derived exosomes confer drug resistance trait to sensitive CML cells. • Exosomes mediate horizontal transfer of miR-365 between imatinib resistant CML cells and sensitive ones. • MiR-365 lowers chemosensitivity and apoptosis in imatinib-sensitive CML cells. Chronic myeloid leukemia (CML) is a malignant disorder of hematopoietic stem/progenitor cells. Majority of patients can be effectively treated with tyrosine kinase inhibitors (TKIs) such as imatinib, but a portion of patients will develop drug resistance. Accumulated evidences have identified exosomes in cancer as promoters of tumor progression. Herein, we found that exosomes derived from imatinib resistant CML cells can be internalized into sensitive CML cells and confer drug-resistance traits. We also demonstrated a significant higher level of miR-365 in exosomes derived from drug-resistant CML cells compared with those from sensitive ones using microarray and qRT-PCR. The imatinib sensitive CML cells transfected with pre-miR-365 displayed lower chemosensitivity and apoptosis rate compared with controls. We further confirmed that exosomal transfer of miR-365 induced drug resistance by inhibiting expression of pro-apoptosis protein in sensitive CML cells. In conclusion, our study reveals that exosomes mediate a horizontal transfer of drug-resistant trait in chronic myeloid leukemia cell by delivering miR-365.},
doi = {10.1016/J.YEXCR.2017.12.001},
url = {https://www.osti.gov/biblio/23082491}, journal = {Experimental Cell Research},
issn = {0014-4827},
number = 2,
volume = 362,
place = {United States},
year = {Mon Jan 15 00:00:00 EST 2018},
month = {Mon Jan 15 00:00:00 EST 2018}
}