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Title: Five Micron High Resolution MALDI Mass Spectrometry Imaging with Simple, Interchangeable, Multi-Resolution Optical System

Abstract

High-spatial resolution mass spectrometry imaging (MSI) is crucial for the mapping of chemical distributions at the cellular and subcellular level. In this work, we improved our previous laser optical system for matrix-assisted laser desorption ionization (MALDI)-MSI, from ~9 μm practical laser spot size to a practical laser spot size of ~4 μm, thereby allowing for 5 μm resolution imaging without oversampling. This is accomplished through a combination of spatial filtering, beam expansion, and reduction of the final focal length. Most importantly, the new laser optics system allows for simple modification of the spot size solely through the interchanging of the beam expander component. Using 10×, 5×, and no beam expander, we could routinely change between ~4, ~7, and ~45 μm laser spot size, in less than 5 min. We applied this multi-resolution MALDI-MSI system to a single maize root tissue section with three different spatial resolutions of 5, 10, and 50 μm and compared the differences in imaging quality and signal sensitivity. We also demonstrated the difference in depth of focus between the optical systems with 10× and 5× beam expanders. .

Authors:
;
Publication Date:
OSTI Identifier:
22777069
Resource Type:
Journal Article
Journal Name:
Journal of the American Society for Mass Spectrometry
Additional Journal Information:
Journal Volume: 28; Journal Issue: 3; Other Information: Copyright (c) 2017 American Society for Mass Spectrometry; http://www.springer-ny.com; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 1044-0305
Country of Publication:
United States
Language:
English
Subject:
46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND TECHNOLOGY; COMPARATIVE EVALUATIONS; DESORPTION; DISTRIBUTION; FILTERS; IONIZATION; LASER RADIATION; MASS SPECTROSCOPY; OPTICAL SYSTEMS; REDUCTION; SPATIAL RESOLUTION

Citation Formats

Feenstra, Adam D., Dueñas, Maria Emilia, and Lee, Young Jin, E-mail: yjlee@iastate.edu. Five Micron High Resolution MALDI Mass Spectrometry Imaging with Simple, Interchangeable, Multi-Resolution Optical System. United States: N. p., 2017. Web. doi:10.1007/S13361-016-1577-8.
Feenstra, Adam D., Dueñas, Maria Emilia, & Lee, Young Jin, E-mail: yjlee@iastate.edu. Five Micron High Resolution MALDI Mass Spectrometry Imaging with Simple, Interchangeable, Multi-Resolution Optical System. United States. doi:10.1007/S13361-016-1577-8.
Feenstra, Adam D., Dueñas, Maria Emilia, and Lee, Young Jin, E-mail: yjlee@iastate.edu. Wed . "Five Micron High Resolution MALDI Mass Spectrometry Imaging with Simple, Interchangeable, Multi-Resolution Optical System". United States. doi:10.1007/S13361-016-1577-8.
@article{osti_22777069,
title = {Five Micron High Resolution MALDI Mass Spectrometry Imaging with Simple, Interchangeable, Multi-Resolution Optical System},
author = {Feenstra, Adam D. and Dueñas, Maria Emilia and Lee, Young Jin, E-mail: yjlee@iastate.edu},
abstractNote = {High-spatial resolution mass spectrometry imaging (MSI) is crucial for the mapping of chemical distributions at the cellular and subcellular level. In this work, we improved our previous laser optical system for matrix-assisted laser desorption ionization (MALDI)-MSI, from ~9 μm practical laser spot size to a practical laser spot size of ~4 μm, thereby allowing for 5 μm resolution imaging without oversampling. This is accomplished through a combination of spatial filtering, beam expansion, and reduction of the final focal length. Most importantly, the new laser optics system allows for simple modification of the spot size solely through the interchanging of the beam expander component. Using 10×, 5×, and no beam expander, we could routinely change between ~4, ~7, and ~45 μm laser spot size, in less than 5 min. We applied this multi-resolution MALDI-MSI system to a single maize root tissue section with three different spatial resolutions of 5, 10, and 50 μm and compared the differences in imaging quality and signal sensitivity. We also demonstrated the difference in depth of focus between the optical systems with 10× and 5× beam expanders. .},
doi = {10.1007/S13361-016-1577-8},
journal = {Journal of the American Society for Mass Spectrometry},
issn = {1044-0305},
number = 3,
volume = 28,
place = {United States},
year = {2017},
month = {3}
}

Works referencing / citing this record:

Fourier Transform Infrared Microscopy Enables Guidance of Automated Mass Spectrometry Imaging to Predefined Tissue Morphologies
journal, January 2018