skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Surface Induced Dissociation Coupled with High Resolution Mass Spectrometry Unveils Heterogeneity of a 211 kDa Multicopper Oxidase Protein Complex

Abstract

Manganese oxidation is an important biogeochemical process that is largely regulated by bacteria through enzymatic reactions. However, the detailed mechanism is poorly understood due to challenges in isolating and characterizing these unknown enzymes. A manganese oxidase, Mnx, from Bacillus sp. PL-12 has been successfully overexpressed in active form as a protein complex with a molecular mass of 211 kDa. We have recently used surface induced dissociation (SID) and ion mobility-mass spectrometry (IM-MS) to release and detect folded subcomplexes for determining subunit connectivity and quaternary structure. The data from the native mass spectrometry experiments led to a plausible structural model of this multicopper oxidase, which has been difficult to study by conventional structural biology methods. It was also revealed that each Mnx subunit binds a variable number of copper ions. Becasue of the heterogeneity of the protein and limited mass resolution, ambiguities in assigning some of the observed peaks remained as a barrier to fully understanding the role of metals and potential unknown ligands in Mnx. In this study, we performed SID in a modified Fourier transform-ion cyclotron resonance (FTICR) mass spectrometer. The high mass accuracy and resolution offered by FTICR unveiled unexpected artificial modifications on the protein that had been previously thoughtmore » to be iron bound species based on lower resolution spectra. Additionally, isotopically resolved spectra of the released subcomplexes revealed the metal binding stoichiometry at different structural levels. This method holds great potential for in-depth characterization of metalloproteins and protein–ligand complexes. .« less

Authors:
 [1];  [2]; ;  [3];  [2];  [1]
  1. Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory (United States)
  2. Ohio State University, Department of Chemistry and Biochemistry (United States)
  3. Oregon Health & Science University, Division of Environmental and Biomolecular Systems (United States)
Publication Date:
OSTI Identifier:
22776929
Resource Type:
Journal Article
Journal Name:
Journal of the American Society for Mass Spectrometry
Additional Journal Information:
Journal Volume: 29; Journal Issue: 4; Other Information: Copyright (c) 2018 American Society for Mass Spectrometry; Article Copyright (c) 2018 American Society for Mass Spectrometry (outside the USA); http://www.springer-ny.com; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 1044-0305
Country of Publication:
United States
Language:
English
Subject:
46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND TECHNOLOGY; ACCURACY; DIFFUSION BARRIERS; FOURIER TRANSFORMATION; INTERACTIONS; ION CYCLOTRON-RESONANCE; ION MOBILITY; MASS RESOLUTION; MASS SPECTROMETERS; MASS SPECTROSCOPY; METALLOPROTEINS; MODIFICATIONS; OXIDASES; SURFACES

Citation Formats

Zhou, Mowei, Yan, Jing, Romano, Christine A., Tebo, Bradley M., Wysocki, Vicki H., and Paša-Tolić, Ljiljana. Surface Induced Dissociation Coupled with High Resolution Mass Spectrometry Unveils Heterogeneity of a 211 kDa Multicopper Oxidase Protein Complex. United States: N. p., 2018. Web. doi:10.1007/S13361-017-1882-X.
Zhou, Mowei, Yan, Jing, Romano, Christine A., Tebo, Bradley M., Wysocki, Vicki H., & Paša-Tolić, Ljiljana. Surface Induced Dissociation Coupled with High Resolution Mass Spectrometry Unveils Heterogeneity of a 211 kDa Multicopper Oxidase Protein Complex. United States. doi:10.1007/S13361-017-1882-X.
Zhou, Mowei, Yan, Jing, Romano, Christine A., Tebo, Bradley M., Wysocki, Vicki H., and Paša-Tolić, Ljiljana. Sun . "Surface Induced Dissociation Coupled with High Resolution Mass Spectrometry Unveils Heterogeneity of a 211 kDa Multicopper Oxidase Protein Complex". United States. doi:10.1007/S13361-017-1882-X.
@article{osti_22776929,
title = {Surface Induced Dissociation Coupled with High Resolution Mass Spectrometry Unveils Heterogeneity of a 211 kDa Multicopper Oxidase Protein Complex},
author = {Zhou, Mowei and Yan, Jing and Romano, Christine A. and Tebo, Bradley M. and Wysocki, Vicki H. and Paša-Tolić, Ljiljana},
abstractNote = {Manganese oxidation is an important biogeochemical process that is largely regulated by bacteria through enzymatic reactions. However, the detailed mechanism is poorly understood due to challenges in isolating and characterizing these unknown enzymes. A manganese oxidase, Mnx, from Bacillus sp. PL-12 has been successfully overexpressed in active form as a protein complex with a molecular mass of 211 kDa. We have recently used surface induced dissociation (SID) and ion mobility-mass spectrometry (IM-MS) to release and detect folded subcomplexes for determining subunit connectivity and quaternary structure. The data from the native mass spectrometry experiments led to a plausible structural model of this multicopper oxidase, which has been difficult to study by conventional structural biology methods. It was also revealed that each Mnx subunit binds a variable number of copper ions. Becasue of the heterogeneity of the protein and limited mass resolution, ambiguities in assigning some of the observed peaks remained as a barrier to fully understanding the role of metals and potential unknown ligands in Mnx. In this study, we performed SID in a modified Fourier transform-ion cyclotron resonance (FTICR) mass spectrometer. The high mass accuracy and resolution offered by FTICR unveiled unexpected artificial modifications on the protein that had been previously thought to be iron bound species based on lower resolution spectra. Additionally, isotopically resolved spectra of the released subcomplexes revealed the metal binding stoichiometry at different structural levels. This method holds great potential for in-depth characterization of metalloproteins and protein–ligand complexes. .},
doi = {10.1007/S13361-017-1882-X},
journal = {Journal of the American Society for Mass Spectrometry},
issn = {1044-0305},
number = 4,
volume = 29,
place = {United States},
year = {2018},
month = {4}
}