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Title: Broad Separation of Isomeric Lipids by High-Resolution Differential Ion Mobility Spectrometry with Tandem Mass Spectrometry

Abstract

Maturation of metabolomics has brought a deeper appreciation for the importance of isomeric identity of lipids to their biological role, mirroring that for proteoforms in proteomics. However, full characterization of the lipid isomerism has been thwarted by paucity of rapid and effective analytical tools. A novel approach is ion mobility spectrometry (IMS) and particularly differential or field asymmetric waveform IMS (FAIMS) at high electric fields, which is more orthogonal to mass spectrometry. Here we broadly explore the power of FAIMS to separate lipid isomers, and find a ~75% success rate across the four major types of glycero- and phospho- lipids (sn, chain length, double bond position, and cis/trans). The resolved isomers were identified using standards, and (for the first two types) tandem mass spectrometry. These results demonstrate the general merit of incorporating high-resolution FAIMS into lipidomic analyses. .

Authors:
 [1];  [2];  [1]
  1. Wichita State University, Department of Chemistry (United States)
  2. City University of New York (United States)
Publication Date:
OSTI Identifier:
22776920
Resource Type:
Journal Article
Journal Name:
Journal of the American Society for Mass Spectrometry
Additional Journal Information:
Journal Volume: 28; Journal Issue: 8; Other Information: Copyright (c) 2017 American Society for Mass Spectrometry; http://www.springer-ny.com; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 1044-0305
Country of Publication:
United States
Language:
English
Subject:
46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND TECHNOLOGY; ASYMMETRY; DOUBLE BONDS; ELECTRIC FIELDS; ION MOBILITY; ISOMERS; LIPIDS; MASS SPECTROSCOPY; RESOLUTION; TOOLS; WAVE FORMS

Citation Formats

Bowman, Andrew P., Abzalimov, Rinat R., and Shvartsburg, Alexandre A., E-mail: alexandre.shvartsburg@wichita.edu. Broad Separation of Isomeric Lipids by High-Resolution Differential Ion Mobility Spectrometry with Tandem Mass Spectrometry. United States: N. p., 2017. Web. doi:10.1007/S13361-017-1675-2.
Bowman, Andrew P., Abzalimov, Rinat R., & Shvartsburg, Alexandre A., E-mail: alexandre.shvartsburg@wichita.edu. Broad Separation of Isomeric Lipids by High-Resolution Differential Ion Mobility Spectrometry with Tandem Mass Spectrometry. United States. doi:10.1007/S13361-017-1675-2.
Bowman, Andrew P., Abzalimov, Rinat R., and Shvartsburg, Alexandre A., E-mail: alexandre.shvartsburg@wichita.edu. Tue . "Broad Separation of Isomeric Lipids by High-Resolution Differential Ion Mobility Spectrometry with Tandem Mass Spectrometry". United States. doi:10.1007/S13361-017-1675-2.
@article{osti_22776920,
title = {Broad Separation of Isomeric Lipids by High-Resolution Differential Ion Mobility Spectrometry with Tandem Mass Spectrometry},
author = {Bowman, Andrew P. and Abzalimov, Rinat R. and Shvartsburg, Alexandre A., E-mail: alexandre.shvartsburg@wichita.edu},
abstractNote = {Maturation of metabolomics has brought a deeper appreciation for the importance of isomeric identity of lipids to their biological role, mirroring that for proteoforms in proteomics. However, full characterization of the lipid isomerism has been thwarted by paucity of rapid and effective analytical tools. A novel approach is ion mobility spectrometry (IMS) and particularly differential or field asymmetric waveform IMS (FAIMS) at high electric fields, which is more orthogonal to mass spectrometry. Here we broadly explore the power of FAIMS to separate lipid isomers, and find a ~75% success rate across the four major types of glycero- and phospho- lipids (sn, chain length, double bond position, and cis/trans). The resolved isomers were identified using standards, and (for the first two types) tandem mass spectrometry. These results demonstrate the general merit of incorporating high-resolution FAIMS into lipidomic analyses. .},
doi = {10.1007/S13361-017-1675-2},
journal = {Journal of the American Society for Mass Spectrometry},
issn = {1044-0305},
number = 8,
volume = 28,
place = {United States},
year = {2017},
month = {8}
}