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Title: Investigation of the Ionization Mechanism of NAD{sup +}/NADH-Modified Gold Electrodes in ToF-SIMS Analysis

Abstract

Analysis of nicotinamide adenine dinucleotide (NAD{sup +}/NADH)-modified electrodes is important for in vitro monitoring of key biological processes. In this work, time-of-flight secondary ion mass spectrometry (ToF-SIMS) was used to analyze NAD{sup +}/NADH-modified gold electrodes. Interestingly, no obvious characteristic peaks of nicotinamide fragment could be observed in the mass spectra of NAD{sup +}/NADH in their neutral sodium pyrophosphate form. However, after acidification, the characteristic peaks for both NAD{sup +} and NADH were detected. This was due to the suppression effect of inner pyrophosphoric salts in both neutral molecules. Besides, it was proved that the suppression by inner salt was intramolecular. No obvious suppression was found between neighboring molecules. These results demonstrated the suppression effect of inner salts in ToF-SIMS analysis, providing useful evidence for the study of ToF-SIMS ionization mechanism of organic molecule-modified electrodes. .

Authors:
; ;  [1]
  1. East China University of Science and Technology, Key Laboratory for Advanced Materials and Department of Chemistry (China)
Publication Date:
OSTI Identifier:
22776868
Resource Type:
Journal Article
Journal Name:
Journal of the American Society for Mass Spectrometry
Additional Journal Information:
Journal Volume: 29; Journal Issue: 8; Other Information: Copyright (c) 2018 American Society for Mass Spectrometry; http://www.springer-ny.com; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 1044-0305
Country of Publication:
United States
Language:
English
Subject:
46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND TECHNOLOGY; GOLD; ION MICROPROBE ANALYSIS; IONIZATION; MASS SPECTRA; MASS SPECTROSCOPY; NICOTINAMIDE; PHOSPHATES; SODIUM; TIME-OF-FLIGHT METHOD

Citation Formats

Hua, Xin, Zhao, Li-Jun, and Long, Yi-Tao. Investigation of the Ionization Mechanism of NAD{sup +}/NADH-Modified Gold Electrodes in ToF-SIMS Analysis. United States: N. p., 2018. Web. doi:10.1007/S13361-018-1983-1.
Hua, Xin, Zhao, Li-Jun, & Long, Yi-Tao. Investigation of the Ionization Mechanism of NAD{sup +}/NADH-Modified Gold Electrodes in ToF-SIMS Analysis. United States. doi:10.1007/S13361-018-1983-1.
Hua, Xin, Zhao, Li-Jun, and Long, Yi-Tao. Wed . "Investigation of the Ionization Mechanism of NAD{sup +}/NADH-Modified Gold Electrodes in ToF-SIMS Analysis". United States. doi:10.1007/S13361-018-1983-1.
@article{osti_22776868,
title = {Investigation of the Ionization Mechanism of NAD{sup +}/NADH-Modified Gold Electrodes in ToF-SIMS Analysis},
author = {Hua, Xin and Zhao, Li-Jun and Long, Yi-Tao},
abstractNote = {Analysis of nicotinamide adenine dinucleotide (NAD{sup +}/NADH)-modified electrodes is important for in vitro monitoring of key biological processes. In this work, time-of-flight secondary ion mass spectrometry (ToF-SIMS) was used to analyze NAD{sup +}/NADH-modified gold electrodes. Interestingly, no obvious characteristic peaks of nicotinamide fragment could be observed in the mass spectra of NAD{sup +}/NADH in their neutral sodium pyrophosphate form. However, after acidification, the characteristic peaks for both NAD{sup +} and NADH were detected. This was due to the suppression effect of inner pyrophosphoric salts in both neutral molecules. Besides, it was proved that the suppression by inner salt was intramolecular. No obvious suppression was found between neighboring molecules. These results demonstrated the suppression effect of inner salts in ToF-SIMS analysis, providing useful evidence for the study of ToF-SIMS ionization mechanism of organic molecule-modified electrodes. .},
doi = {10.1007/S13361-018-1983-1},
journal = {Journal of the American Society for Mass Spectrometry},
issn = {1044-0305},
number = 8,
volume = 29,
place = {United States},
year = {2018},
month = {8}
}