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Title: Biocrystallization in Bacterial and Fungal Cells and Spores

Abstract

A series of X-ray diffraction experiments were performed for the first time to study stress-induced biocrystallization (structural response to stress) in the bacteria E. coli, the spore-forming bacteria Bacillus cereus, and in cells and spores of the mycelial fungus Umbelopsis ramanniana. High-intensity areas with spacings of 90 and 44 Å are indicative of a periodically ordered arrangement (most likely nanocrystalline) of the bacterial nucleoid. For the starved bacteria Bacillus cereus, a peak at a spacing of 45 Å is also assigned to nanocrystalline complexes of DNA with the Dps protein. The spores of the fungus Umbelopsis ramanniana VKM F-582, as well as the spores of Bacillus cereus, form ordered arrays of DNA molecules with DNA-condensing acid-soluble proteins SASPs. Starved dehydrated mycelial cells of the fungus Umbelopsis ramanniana form ordered structures with spacings from 27 to 55 Å. A series of peaks reflect the formation of a number of ordered protein arrays, apparently with DNA, with continuously varying characteristic interplanar spacings.

Authors:
; ; ; ; ; ;  [1]; ; ; ; ;  [2]; ;  [3];  [4]
  1. Russian Academy of Sciences, Semenov Institute of Chemical Physics (Russian Federation)
  2. Russian Academy of Sciences, Federal Research Centre “Fundamentals of Biotechnology,” (Russian Federation)
  3. Moscow State University (Russian Federation)
  4. European Synchrotron Radiation Facility (France)
Publication Date:
OSTI Identifier:
22758218
Resource Type:
Journal Article
Journal Name:
Crystallography Reports
Additional Journal Information:
Journal Volume: 63; Journal Issue: 4; Other Information: Copyright (c) 2018 Pleiades Publishing, Inc.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 1063-7745
Country of Publication:
United States
Language:
English
Subject:
75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY; 37 INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY; 60 APPLIED LIFE SCIENCES; BACILLUS CEREUS; CRYSTALS; DNA; FUNGI; MOLECULES; NANOSTRUCTURES; PEAKS; PERIODICITY; PROTEINS; SPORES; STRESSES; X-RAY DIFFRACTION

Citation Formats

Krupyanskii, Y. F., E-mail: yufk@chph.ras.ru, Loiko, N. G., Sinitsyn, D. O., Tereshkina, K. B., Tereshkin, E. V., Frolov, I. A., Chulichkov, A. L., Bokareva, D. A., Mysyakina, I. S., Nikolaev, Y. A., El’-Registan, G. I., Popov, V. O., Sokolova, O. S., Shaitan, K. V., and Popov, A. N. Biocrystallization in Bacterial and Fungal Cells and Spores. United States: N. p., 2018. Web. doi:10.1134/S1063774518040144.
Krupyanskii, Y. F., E-mail: yufk@chph.ras.ru, Loiko, N. G., Sinitsyn, D. O., Tereshkina, K. B., Tereshkin, E. V., Frolov, I. A., Chulichkov, A. L., Bokareva, D. A., Mysyakina, I. S., Nikolaev, Y. A., El’-Registan, G. I., Popov, V. O., Sokolova, O. S., Shaitan, K. V., & Popov, A. N. Biocrystallization in Bacterial and Fungal Cells and Spores. United States. doi:10.1134/S1063774518040144.
Krupyanskii, Y. F., E-mail: yufk@chph.ras.ru, Loiko, N. G., Sinitsyn, D. O., Tereshkina, K. B., Tereshkin, E. V., Frolov, I. A., Chulichkov, A. L., Bokareva, D. A., Mysyakina, I. S., Nikolaev, Y. A., El’-Registan, G. I., Popov, V. O., Sokolova, O. S., Shaitan, K. V., and Popov, A. N. Sun . "Biocrystallization in Bacterial and Fungal Cells and Spores". United States. doi:10.1134/S1063774518040144.
@article{osti_22758218,
title = {Biocrystallization in Bacterial and Fungal Cells and Spores},
author = {Krupyanskii, Y. F., E-mail: yufk@chph.ras.ru and Loiko, N. G. and Sinitsyn, D. O. and Tereshkina, K. B. and Tereshkin, E. V. and Frolov, I. A. and Chulichkov, A. L. and Bokareva, D. A. and Mysyakina, I. S. and Nikolaev, Y. A. and El’-Registan, G. I. and Popov, V. O. and Sokolova, O. S. and Shaitan, K. V. and Popov, A. N.},
abstractNote = {A series of X-ray diffraction experiments were performed for the first time to study stress-induced biocrystallization (structural response to stress) in the bacteria E. coli, the spore-forming bacteria Bacillus cereus, and in cells and spores of the mycelial fungus Umbelopsis ramanniana. High-intensity areas with spacings of 90 and 44 Å are indicative of a periodically ordered arrangement (most likely nanocrystalline) of the bacterial nucleoid. For the starved bacteria Bacillus cereus, a peak at a spacing of 45 Å is also assigned to nanocrystalline complexes of DNA with the Dps protein. The spores of the fungus Umbelopsis ramanniana VKM F-582, as well as the spores of Bacillus cereus, form ordered arrays of DNA molecules with DNA-condensing acid-soluble proteins SASPs. Starved dehydrated mycelial cells of the fungus Umbelopsis ramanniana form ordered structures with spacings from 27 to 55 Å. A series of peaks reflect the formation of a number of ordered protein arrays, apparently with DNA, with continuously varying characteristic interplanar spacings.},
doi = {10.1134/S1063774518040144},
journal = {Crystallography Reports},
issn = {1063-7745},
number = 4,
volume = 63,
place = {United States},
year = {2018},
month = {7}
}