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Title: PSME3 induces epithelial–mesenchymal transition with inducing the expression of CSC markers and immunosuppression in breast cancer

Abstract

Proteasome activator subunit 3 (PSME3) plays a key role in breast cancer by regulating the cell cycle. However, its role in other pathogenesis-related features of breast cancer is unclear. In this study, we found that overexpression of PSME3 induced the epithelial–mesenchymal transition and contributed to induce the expression of cancer stem cell markers of the MDA-MB-231 cell line, thus increasing the migration, and invasion of the cells. Moreover, overexpression of PSME3 reduced the chemotaxis of CD8{sup +} T cells and induced the apoptosis of T cells in vitro. Furthermore, PSME3 knockdown increased the number of CD8{sup +} T cells in vivo and reduced the subcutaneous tumor growth rate. These findings revealed that PSME3 induces epithelial–mesenchymal transition with inducing the expression of CSC markers and influencing the tumor immune microenvironment in breast cancer. - Highlights: • PSME3 induces the epithelial–mesenchymal transition in the MDA-MB-231 cell line. • PSME3 promotes the migration, invasion, and proliferation of MDA-MB-231 cells. • PSME3 induces the expression of CSC markers of the MDA-MB-231 cell line. • PSME3 expression induces T-cell apoptosis and increases subcutaneous tumor growth.

Authors:
; ;  [1];  [2];  [3];  [1]
  1. Department of Endocrine and Breast Surgery, First Affiliated Hospital of Chongqing Medical University, Chongqing (China)
  2. Department of Thoracic Surgery, State Key Laboratory of Biotherapy/Collaborative Innovation Center for Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan Province (China)
  3. Department of Thoracic Oncology, State Key Laboratory of Biotherapy/Collaborative Innovation Center for Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan Province (China)
Publication Date:
OSTI Identifier:
22738151
Resource Type:
Journal Article
Journal Name:
Experimental Cell Research
Additional Journal Information:
Journal Volume: 358; Journal Issue: 2; Other Information: Copyright (c) 2017 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0014-4827
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; CELL CYCLE; MAMMARY GLANDS; NEOPLASMS; PLANT GROWTH; STEM CELLS

Citation Formats

Yi, Ziying, Yang, Dejuan, Liao, Xuelian, Guo, Fuchun, Wang, Yongsheng, and Wang, Xiaoyi. PSME3 induces epithelial–mesenchymal transition with inducing the expression of CSC markers and immunosuppression in breast cancer. United States: N. p., 2017. Web. doi:10.1016/J.YEXCR.2017.05.017.
Yi, Ziying, Yang, Dejuan, Liao, Xuelian, Guo, Fuchun, Wang, Yongsheng, & Wang, Xiaoyi. PSME3 induces epithelial–mesenchymal transition with inducing the expression of CSC markers and immunosuppression in breast cancer. United States. doi:10.1016/J.YEXCR.2017.05.017.
Yi, Ziying, Yang, Dejuan, Liao, Xuelian, Guo, Fuchun, Wang, Yongsheng, and Wang, Xiaoyi. Fri . "PSME3 induces epithelial–mesenchymal transition with inducing the expression of CSC markers and immunosuppression in breast cancer". United States. doi:10.1016/J.YEXCR.2017.05.017.
@article{osti_22738151,
title = {PSME3 induces epithelial–mesenchymal transition with inducing the expression of CSC markers and immunosuppression in breast cancer},
author = {Yi, Ziying and Yang, Dejuan and Liao, Xuelian and Guo, Fuchun and Wang, Yongsheng and Wang, Xiaoyi},
abstractNote = {Proteasome activator subunit 3 (PSME3) plays a key role in breast cancer by regulating the cell cycle. However, its role in other pathogenesis-related features of breast cancer is unclear. In this study, we found that overexpression of PSME3 induced the epithelial–mesenchymal transition and contributed to induce the expression of cancer stem cell markers of the MDA-MB-231 cell line, thus increasing the migration, and invasion of the cells. Moreover, overexpression of PSME3 reduced the chemotaxis of CD8{sup +} T cells and induced the apoptosis of T cells in vitro. Furthermore, PSME3 knockdown increased the number of CD8{sup +} T cells in vivo and reduced the subcutaneous tumor growth rate. These findings revealed that PSME3 induces epithelial–mesenchymal transition with inducing the expression of CSC markers and influencing the tumor immune microenvironment in breast cancer. - Highlights: • PSME3 induces the epithelial–mesenchymal transition in the MDA-MB-231 cell line. • PSME3 promotes the migration, invasion, and proliferation of MDA-MB-231 cells. • PSME3 induces the expression of CSC markers of the MDA-MB-231 cell line. • PSME3 expression induces T-cell apoptosis and increases subcutaneous tumor growth.},
doi = {10.1016/J.YEXCR.2017.05.017},
journal = {Experimental Cell Research},
issn = {0014-4827},
number = 2,
volume = 358,
place = {United States},
year = {2017},
month = {9}
}