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Title: Two single mutations in the fusion protein of Newcastle disease virus confer hemagglutinin-neuraminidase independent fusion promotion and attenuate the pathogenicity in chickens

Abstract

The fusion (F) protein of Newcastle disease virus (NDV) affects viral infection and pathogenicity through mediating membrane fusion. Previously, we found NDV with increased fusogenic activity in which contained T458D or G459D mutation in the F protein. Here, we investigated the effects of these two mutations on viral infection, fusogenicity and pathogenicity. Syncytium formation assays indicated that T458D or G459D increased the F protein cleavage activity and enhanced cell fusion with or without the presence of HN protein. The T458D- or G459D-mutated NDV resulted in a decrease in virus replication or release from cells. The animal study showed that the pathogenicity of the mutated NDVs was attenuated in chickens. These results indicate that these two single mutations in F altered or diminished the requirement of HN for promoting membrane fusion. The increased fusogenic activity may disrupt the cellular machinery and consequently decrease the virus replication and pathogenicity in chickens. - Highlights: • T458D or G459D mutation in the F protein enhanced the fuosogenicity with HN. • T458D or G459D mutation in the F protein promoted the fusion independently. • The growth of the T458D or G459D mutated virus was decreased in the cells. • The pathogenicity of the T458D ormore » G459D mutated virus was attenuated in chickens.« less

Authors:
; ; ;  [1];  [2]; ;  [1];  [1];  [1]
  1. State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046 (China)
  2. United States Department of Agriculture, Agriculture Research Service, US National Poultry Research Center, Southeast Poultry Research Laboratory, Athens, GA 30605 (United States)
Publication Date:
OSTI Identifier:
22722965
Resource Type:
Journal Article
Journal Name:
Virology
Additional Journal Information:
Journal Volume: 509; Other Information: Copyright (c) 2017 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0042-6822
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; CHICKENS; MUTANTS; MUTATIONS; NEWCASTLE DISEASE; PLANT GROWTH; VIRUSES

Citation Formats

Ji, Yanhong, Liu, Tao, Jia, Yane, Liu, Bin, Yu, Qingzhong, Cui, Xiaole, Guo, Fengfeng, Chang, Huiyun, and Zhu, Qiyun. Two single mutations in the fusion protein of Newcastle disease virus confer hemagglutinin-neuraminidase independent fusion promotion and attenuate the pathogenicity in chickens. United States: N. p., 2017. Web. doi:10.1016/J.VIROL.2017.06.021.
Ji, Yanhong, Liu, Tao, Jia, Yane, Liu, Bin, Yu, Qingzhong, Cui, Xiaole, Guo, Fengfeng, Chang, Huiyun, & Zhu, Qiyun. Two single mutations in the fusion protein of Newcastle disease virus confer hemagglutinin-neuraminidase independent fusion promotion and attenuate the pathogenicity in chickens. United States. https://doi.org/10.1016/J.VIROL.2017.06.021
Ji, Yanhong, Liu, Tao, Jia, Yane, Liu, Bin, Yu, Qingzhong, Cui, Xiaole, Guo, Fengfeng, Chang, Huiyun, and Zhu, Qiyun. Fri . "Two single mutations in the fusion protein of Newcastle disease virus confer hemagglutinin-neuraminidase independent fusion promotion and attenuate the pathogenicity in chickens". United States. https://doi.org/10.1016/J.VIROL.2017.06.021.
@article{osti_22722965,
title = {Two single mutations in the fusion protein of Newcastle disease virus confer hemagglutinin-neuraminidase independent fusion promotion and attenuate the pathogenicity in chickens},
author = {Ji, Yanhong and Liu, Tao and Jia, Yane and Liu, Bin and Yu, Qingzhong and Cui, Xiaole and Guo, Fengfeng and Chang, Huiyun and Zhu, Qiyun},
abstractNote = {The fusion (F) protein of Newcastle disease virus (NDV) affects viral infection and pathogenicity through mediating membrane fusion. Previously, we found NDV with increased fusogenic activity in which contained T458D or G459D mutation in the F protein. Here, we investigated the effects of these two mutations on viral infection, fusogenicity and pathogenicity. Syncytium formation assays indicated that T458D or G459D increased the F protein cleavage activity and enhanced cell fusion with or without the presence of HN protein. The T458D- or G459D-mutated NDV resulted in a decrease in virus replication or release from cells. The animal study showed that the pathogenicity of the mutated NDVs was attenuated in chickens. These results indicate that these two single mutations in F altered or diminished the requirement of HN for promoting membrane fusion. The increased fusogenic activity may disrupt the cellular machinery and consequently decrease the virus replication and pathogenicity in chickens. - Highlights: • T458D or G459D mutation in the F protein enhanced the fuosogenicity with HN. • T458D or G459D mutation in the F protein promoted the fusion independently. • The growth of the T458D or G459D mutated virus was decreased in the cells. • The pathogenicity of the T458D or G459D mutated virus was attenuated in chickens.},
doi = {10.1016/J.VIROL.2017.06.021},
url = {https://www.osti.gov/biblio/22722965}, journal = {Virology},
issn = {0042-6822},
number = ,
volume = 509,
place = {United States},
year = {2017},
month = {9}
}