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Title: A Sendai virus recombinant vaccine expressing a gene for truncated human metapneumovirus (hMPV) fusion protein protects cotton rats from hMPV challenge

Abstract

Human metapneumovirus (hMPV) infections pose a serious health risk to young children, particularly in cases of premature birth. No licensed vaccine exists and there is no standard treatment for hMPV infections apart from supportive hospital care. We describe the production of a Sendai virus (SeV) recombinant that carries a gene for a truncated hMPV fusion (F) protein (SeV-MPV-Ft). The vaccine induces binding and neutralizing antibody responses toward hMPV and protection against challenge with hMPV in a cotton rat system. Results encourage advanced development of SeV-MPV-Ft to prevent the morbidity and mortality caused by hMPV infections in young children. - Highlights: • A Sendai virus-based human metapneumovirus (hMPV) vaccine is being developed. • SeV-MPV-Ft carries a gene for a truncated metapneumovirus fusion protein. • The intranasal vaccine induces hMPV-specific binding antibodies in cotton rats. • The vaccine induces hMPV-specific neutralizing antibodies in cotton rats. • A single immunization protects against hMPV challenge in vivo.

Authors:
 [1];  [2]; ; ; ;  [1];  [3];  [4];  [5];  [1];  [2]
  1. Department of Infectious Diseases, St. Jude Children’s Research Hospital, 262 Danny Thomas Place, Memphis, TN 38105 (United States)
  2. (United States)
  3. Department of Pathology, St. Jude Children's Research Hospital (United States)
  4. Department of Infectious Diseases, College of Veterinary Medicine, The University of Georgia, Athens, GA (United States)
  5. University of Rochester, Rochester, NY (United States)
Publication Date:
OSTI Identifier:
22722963
Resource Type:
Journal Article
Journal Name:
Virology
Additional Journal Information:
Journal Volume: 509; Other Information: Copyright (c) 2017 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0042-6822
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; COTTON; DISEASE INCIDENCE; GENES; HEALTH HAZARDS; RATS; SAFETY; VACCINES; VIRUSES

Citation Formats

Russell, Charles J., Department of Microbiology, Immunology and Biochemistry, University of Tennessee Health Science Center, Memphis, TN, Jones, Bart G., Sealy, Robert E., Surman, Sherri L., Mason, John N., Hayden, Randall T., Tripp, Ralph A., Takimoto, Toru, Hurwitz, Julia L., E-mail: julia.hurwitz@stjude.org, and Department of Microbiology, Immunology and Biochemistry, University of Tennessee Health Science Center, Memphis, TN. A Sendai virus recombinant vaccine expressing a gene for truncated human metapneumovirus (hMPV) fusion protein protects cotton rats from hMPV challenge. United States: N. p., 2017. Web. doi:10.1016/J.VIROL.2017.05.021.
Russell, Charles J., Department of Microbiology, Immunology and Biochemistry, University of Tennessee Health Science Center, Memphis, TN, Jones, Bart G., Sealy, Robert E., Surman, Sherri L., Mason, John N., Hayden, Randall T., Tripp, Ralph A., Takimoto, Toru, Hurwitz, Julia L., E-mail: julia.hurwitz@stjude.org, & Department of Microbiology, Immunology and Biochemistry, University of Tennessee Health Science Center, Memphis, TN. A Sendai virus recombinant vaccine expressing a gene for truncated human metapneumovirus (hMPV) fusion protein protects cotton rats from hMPV challenge. United States. doi:10.1016/J.VIROL.2017.05.021.
Russell, Charles J., Department of Microbiology, Immunology and Biochemistry, University of Tennessee Health Science Center, Memphis, TN, Jones, Bart G., Sealy, Robert E., Surman, Sherri L., Mason, John N., Hayden, Randall T., Tripp, Ralph A., Takimoto, Toru, Hurwitz, Julia L., E-mail: julia.hurwitz@stjude.org, and Department of Microbiology, Immunology and Biochemistry, University of Tennessee Health Science Center, Memphis, TN. Fri . "A Sendai virus recombinant vaccine expressing a gene for truncated human metapneumovirus (hMPV) fusion protein protects cotton rats from hMPV challenge". United States. doi:10.1016/J.VIROL.2017.05.021.
@article{osti_22722963,
title = {A Sendai virus recombinant vaccine expressing a gene for truncated human metapneumovirus (hMPV) fusion protein protects cotton rats from hMPV challenge},
author = {Russell, Charles J. and Department of Microbiology, Immunology and Biochemistry, University of Tennessee Health Science Center, Memphis, TN and Jones, Bart G. and Sealy, Robert E. and Surman, Sherri L. and Mason, John N. and Hayden, Randall T. and Tripp, Ralph A. and Takimoto, Toru and Hurwitz, Julia L., E-mail: julia.hurwitz@stjude.org and Department of Microbiology, Immunology and Biochemistry, University of Tennessee Health Science Center, Memphis, TN},
abstractNote = {Human metapneumovirus (hMPV) infections pose a serious health risk to young children, particularly in cases of premature birth. No licensed vaccine exists and there is no standard treatment for hMPV infections apart from supportive hospital care. We describe the production of a Sendai virus (SeV) recombinant that carries a gene for a truncated hMPV fusion (F) protein (SeV-MPV-Ft). The vaccine induces binding and neutralizing antibody responses toward hMPV and protection against challenge with hMPV in a cotton rat system. Results encourage advanced development of SeV-MPV-Ft to prevent the morbidity and mortality caused by hMPV infections in young children. - Highlights: • A Sendai virus-based human metapneumovirus (hMPV) vaccine is being developed. • SeV-MPV-Ft carries a gene for a truncated metapneumovirus fusion protein. • The intranasal vaccine induces hMPV-specific binding antibodies in cotton rats. • The vaccine induces hMPV-specific neutralizing antibodies in cotton rats. • A single immunization protects against hMPV challenge in vivo.},
doi = {10.1016/J.VIROL.2017.05.021},
journal = {Virology},
issn = {0042-6822},
number = ,
volume = 509,
place = {United States},
year = {2017},
month = {9}
}