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Title: Comparison of the toxicity of sintered and unsintered indium-tin oxide particles in murine macrophage and epidermal cells

Abstract

Indium-tin oxide (ITO) is used to produce flat panel displays and several other technology products. Composed of 90% indium oxide (In{sub 2}O{sub 3}) and 10% tin oxide (SnO{sub 2}) by weight, ITO is synthesized under conditions of high heat via a process known as sintering. Indium lung disease, a recently recognized occupational illness, is characterized by pulmonary alveolar proteinosis, fibrosis, and emphysema. Murine macrophage (RAW 264.7) and epidermal (JB6) cells stably transfected with AP-1 to study tumor promoting potential, were used to differentiate between the toxicological profiles of sintered ITO (SITO) and unsintered mixture (UITO). We hypothesized that sintering would play a key role in free radical generation and cytotoxicity. Exposure of cells to both UITO and SITO caused a time and dose dependent decrease of the viability of cells. Intracellular ROS generation was inversely related to the dose of both UITO and SITO, a direct reflection of the decreased number of viable RAW 264.7 and JB6/AP-1 cells observed at higher concentrations. Electron spin resonance showed significantly increased hydroxyl radical (·OH) generation in cells exposed to UITO compared to SITO. This is different from LDH release, which showed that SITO caused significantly increased damage to the cell membrane compared tomore » UITO. Lastly, the JB6/AP-1 cell line did not show activation of the AP-1 pathway. Our results highlight both the differences in the mechanisms of cytotoxicity and the consistent adverse effects associated with UITO and SITO exposure. - Highlights: • Sintered indium-tin oxide significantly impacts cellular viability and LDH release. • Unsintered indium-tin oxide impacts free radical production and DNA damage. • Indium compounds do not activate the AP-1 pathway.« less

Authors:
 [1]; ;  [1];  [2]; ;  [1];  [2];  [1]
  1. Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Morgantown, WV (United States)
  2. Respiratory Health Division, National Institute for Occupational Safety and Health, Morgantown, WV (United States)
Publication Date:
OSTI Identifier:
22722923
Resource Type:
Journal Article
Journal Name:
Toxicology and Applied Pharmacology
Additional Journal Information:
Journal Volume: 331; Conference: 9. conference on recent advances in metal toxicity and carcinogenesis research, Lexington, KY (United States), 1 Oct 2016; Other Information: Copyright (c) 2017 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0041-008X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; CELL MEMBRANES; DNA DAMAGES; ELECTRON SPIN RESONANCE; GENES; HYDROXYL RADICALS; INDIUM OXIDES; MACROPHAGES; METALS; SINTERING; TOXICITY

Citation Formats

Olgun, Nicole S., E-mail: nolgun@cdc.gov, Morris, Anna M., Barber, Tabatha Lynn, Stefaniak, Aleksandr B., Kashon, Michael L., Schwegler-Berry, Diane, Cummings, Kristin J., and Leonard, Stephen S. Comparison of the toxicity of sintered and unsintered indium-tin oxide particles in murine macrophage and epidermal cells. United States: N. p., 2017. Web. doi:10.1016/J.TAAP.2017.05.028.
Olgun, Nicole S., E-mail: nolgun@cdc.gov, Morris, Anna M., Barber, Tabatha Lynn, Stefaniak, Aleksandr B., Kashon, Michael L., Schwegler-Berry, Diane, Cummings, Kristin J., & Leonard, Stephen S. Comparison of the toxicity of sintered and unsintered indium-tin oxide particles in murine macrophage and epidermal cells. United States. doi:10.1016/J.TAAP.2017.05.028.
Olgun, Nicole S., E-mail: nolgun@cdc.gov, Morris, Anna M., Barber, Tabatha Lynn, Stefaniak, Aleksandr B., Kashon, Michael L., Schwegler-Berry, Diane, Cummings, Kristin J., and Leonard, Stephen S. Fri . "Comparison of the toxicity of sintered and unsintered indium-tin oxide particles in murine macrophage and epidermal cells". United States. doi:10.1016/J.TAAP.2017.05.028.
@article{osti_22722923,
title = {Comparison of the toxicity of sintered and unsintered indium-tin oxide particles in murine macrophage and epidermal cells},
author = {Olgun, Nicole S., E-mail: nolgun@cdc.gov and Morris, Anna M. and Barber, Tabatha Lynn and Stefaniak, Aleksandr B. and Kashon, Michael L. and Schwegler-Berry, Diane and Cummings, Kristin J. and Leonard, Stephen S.},
abstractNote = {Indium-tin oxide (ITO) is used to produce flat panel displays and several other technology products. Composed of 90% indium oxide (In{sub 2}O{sub 3}) and 10% tin oxide (SnO{sub 2}) by weight, ITO is synthesized under conditions of high heat via a process known as sintering. Indium lung disease, a recently recognized occupational illness, is characterized by pulmonary alveolar proteinosis, fibrosis, and emphysema. Murine macrophage (RAW 264.7) and epidermal (JB6) cells stably transfected with AP-1 to study tumor promoting potential, were used to differentiate between the toxicological profiles of sintered ITO (SITO) and unsintered mixture (UITO). We hypothesized that sintering would play a key role in free radical generation and cytotoxicity. Exposure of cells to both UITO and SITO caused a time and dose dependent decrease of the viability of cells. Intracellular ROS generation was inversely related to the dose of both UITO and SITO, a direct reflection of the decreased number of viable RAW 264.7 and JB6/AP-1 cells observed at higher concentrations. Electron spin resonance showed significantly increased hydroxyl radical (·OH) generation in cells exposed to UITO compared to SITO. This is different from LDH release, which showed that SITO caused significantly increased damage to the cell membrane compared to UITO. Lastly, the JB6/AP-1 cell line did not show activation of the AP-1 pathway. Our results highlight both the differences in the mechanisms of cytotoxicity and the consistent adverse effects associated with UITO and SITO exposure. - Highlights: • Sintered indium-tin oxide significantly impacts cellular viability and LDH release. • Unsintered indium-tin oxide impacts free radical production and DNA damage. • Indium compounds do not activate the AP-1 pathway.},
doi = {10.1016/J.TAAP.2017.05.028},
journal = {Toxicology and Applied Pharmacology},
issn = {0041-008X},
number = ,
volume = 331,
place = {United States},
year = {2017},
month = {9}
}