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Title: Maternal exposure to a mixture of persistent organic pollutants (POPs) affects testis histology, epididymal sperm count and induces sperm DNA fragmentation in mice

Abstract

Persistent organic pollutants (POPs) are widespread throughout the environment and some are suspected to induce reproductive toxicity. As animals and humans are exposed to complex mixtures of POPs, it is reasonable to assess how such mixtures could interact with the reproductive system. Our aim is to investigate how maternal exposure to a mixture of 29 different persistent organic pollutants, formulated to mimic the relative POP levels in the food basket of the Scandinavian population, could alter reproductive endpoints. Female mice were exposed via feed from weaning, during pregnancy and lactation in 3 exposure groups (control (C), low (L) and high (H)). Testicular morphometric endpoints, epididymal sperm concentration and sperm DNA integrity were assessed in adult male offspring. We found that the number of tubules, proportion of tubule compartments and epididymal sperm concentration significantly decreased in both POP exposed groups. Epididymal sperm from both POP exposed groups showed increased DNA fragmentation. It is concluded that maternal exposure to a defined POP mixture relevant to human exposure can affect testicular development, sperm production and sperm chromatin integrity. - Highlights: • Male mice were maternally exposed to a mixture of persistent organic pollutants. • Exposure significantly affected the testis histology. • Exposure significantlymore » decreased sperm count and induced sperm DNA fragmentation. • Maternal exposure to POP mixture induced reproductive toxicity in male offspring.« less

Authors:
 [1];  [2];  [3];  [4];  [1];  [3]
  1. Department of Basic Science and Aquatic Medicine, Faculty of Veterinary Medicine, Norwegian University of Life Science, Pb. 8146 Dep, 0033 Oslo (Norway)
  2. Department of Toxicology and Risk, Norwegian Institute of Public Health, Pb 4404, 0403 Oslo (Norway)
  3. Department of Production Animal Clinical Science, Faculty of Veterinary Medicine, Norwegian University of Life Science, Pb. 8146 Dep, 0033 Oslo (Norway)
  4. Norwegian National Institute of Occupational Health, Pb. 8149 Dep, 0033 Oslo (Norway)
Publication Date:
OSTI Identifier:
22722905
Resource Type:
Journal Article
Journal Name:
Toxicology and Applied Pharmacology
Additional Journal Information:
Journal Volume: 329; Other Information: Copyright (c) 2017 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0041-008X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; FRAGMENTATION; HISTOLOGY; MICE; MIXTURES; POLLUTANTS; SPERMATOZOA; TESTES

Citation Formats

Khezri, Abdolrahman, Lindeman, Birgitte, Krogenæs, Anette K., E-mail: Anette.Krogenaes@nmbu.no, Berntsen, Hanne F., E-mail: Hanne.Berntsen@stami.no, Zimmer, Karin E., E-mail: Karin.Zimmer@nmbu.no, and Ropstad, Erik. Maternal exposure to a mixture of persistent organic pollutants (POPs) affects testis histology, epididymal sperm count and induces sperm DNA fragmentation in mice. United States: N. p., 2017. Web. doi:10.1016/J.TAAP.2017.06.019.
Khezri, Abdolrahman, Lindeman, Birgitte, Krogenæs, Anette K., E-mail: Anette.Krogenaes@nmbu.no, Berntsen, Hanne F., E-mail: Hanne.Berntsen@stami.no, Zimmer, Karin E., E-mail: Karin.Zimmer@nmbu.no, & Ropstad, Erik. Maternal exposure to a mixture of persistent organic pollutants (POPs) affects testis histology, epididymal sperm count and induces sperm DNA fragmentation in mice. United States. doi:10.1016/J.TAAP.2017.06.019.
Khezri, Abdolrahman, Lindeman, Birgitte, Krogenæs, Anette K., E-mail: Anette.Krogenaes@nmbu.no, Berntsen, Hanne F., E-mail: Hanne.Berntsen@stami.no, Zimmer, Karin E., E-mail: Karin.Zimmer@nmbu.no, and Ropstad, Erik. Tue . "Maternal exposure to a mixture of persistent organic pollutants (POPs) affects testis histology, epididymal sperm count and induces sperm DNA fragmentation in mice". United States. doi:10.1016/J.TAAP.2017.06.019.
@article{osti_22722905,
title = {Maternal exposure to a mixture of persistent organic pollutants (POPs) affects testis histology, epididymal sperm count and induces sperm DNA fragmentation in mice},
author = {Khezri, Abdolrahman and Lindeman, Birgitte and Krogenæs, Anette K., E-mail: Anette.Krogenaes@nmbu.no and Berntsen, Hanne F., E-mail: Hanne.Berntsen@stami.no and Zimmer, Karin E., E-mail: Karin.Zimmer@nmbu.no and Ropstad, Erik},
abstractNote = {Persistent organic pollutants (POPs) are widespread throughout the environment and some are suspected to induce reproductive toxicity. As animals and humans are exposed to complex mixtures of POPs, it is reasonable to assess how such mixtures could interact with the reproductive system. Our aim is to investigate how maternal exposure to a mixture of 29 different persistent organic pollutants, formulated to mimic the relative POP levels in the food basket of the Scandinavian population, could alter reproductive endpoints. Female mice were exposed via feed from weaning, during pregnancy and lactation in 3 exposure groups (control (C), low (L) and high (H)). Testicular morphometric endpoints, epididymal sperm concentration and sperm DNA integrity were assessed in adult male offspring. We found that the number of tubules, proportion of tubule compartments and epididymal sperm concentration significantly decreased in both POP exposed groups. Epididymal sperm from both POP exposed groups showed increased DNA fragmentation. It is concluded that maternal exposure to a defined POP mixture relevant to human exposure can affect testicular development, sperm production and sperm chromatin integrity. - Highlights: • Male mice were maternally exposed to a mixture of persistent organic pollutants. • Exposure significantly affected the testis histology. • Exposure significantly decreased sperm count and induced sperm DNA fragmentation. • Maternal exposure to POP mixture induced reproductive toxicity in male offspring.},
doi = {10.1016/J.TAAP.2017.06.019},
journal = {Toxicology and Applied Pharmacology},
issn = {0041-008X},
number = ,
volume = 329,
place = {United States},
year = {2017},
month = {8}
}