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Title: The normal trachea is cleaned by MUC5B mucin bundles from the submucosal glands coated with the MUC5AC mucin

Abstract

To understand the mucociliary clearance system, mucins were visualized by light, confocal and electron microscopy, and mucus was stained by Alcian blue and tracked by video microscopy on tracheal explants of newborn piglets. We observed long linear mucus bundles that appeared at the submucosal gland openings and were transported cephalically. The mucus bundles were shown by mass spectrometry and immunostaining to have a core made of MUC5B mucin and were coated with MUC5AC mucin produced by surface goblet cells. The transport speed of the bundles was slower than the airway surface liquid flow. We suggest that the goblet cell MUC5AC mucin anchors the mucus bundles and thus controls their transport. Normal clearance of the respiratory tree of pigs and humans, both rich in submucosal glands, is performed by thick and long mucus bundles. - Highlights: • Submucosal glands in the piglet trachea form bundles of MUC5B mucin. • The mucus bundles are coated with MUC5AC mucin produced by surface goblet cells. • The mucus bundles are transported 10-times slower than the airway surface liquid. • The surface goblet cells are suggested to control the mucus bundle movement.

Authors:
; ;  [1];  [2];  [1];  [3];  [1]; ;  [4];  [5];  [6];  [2];  [1]
  1. Department of Medical Biochemistry, University of Gothenburg, SE-405 30 Gothenburg (Sweden)
  2. Institute of Molecular Animal Breeding and Biotechnology, Gene Center, Ludwig-Maximilians-University Munich, Feodor-Lynen-Straße 25, 81377 Munich (Germany)
  3. (Sweden)
  4. Wellcome Trust Centre for Cell-Matrix Research, Faculty of Biology, Medicine and Health, University of Manchester, Manchester (United Kingdom)
  5. The Cystic Fibrosis Research Laboratory, Stanford University, Stanford, CA 94305 (United States)
  6. Department of Biosciences and Nutrition, Karolinska Institutet, and School of Technology and Health, KTH Royal Institute of Technology, Novum, SE-141 57 Huddinge (Sweden)
Publication Date:
OSTI Identifier:
22719109
Resource Type:
Journal Article
Journal Name:
Biochemical and Biophysical Research Communications
Additional Journal Information:
Journal Volume: 492; Journal Issue: 3; Other Information: Copyright (c) 2017 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; CLEARANCE; ELECTRON MICROSCOPY; GLANDS; INFANTS; LIQUID FLOW; LIQUIDS; LUNGS; MASS SPECTROSCOPY; SWINE; TRACHEA

Citation Formats

Ermund, Anna, Meiss, Lauren N., Rodriguez-Pineiro, Ana M., Bähr, Andrea, Nilsson, Harriet E., Department of Biosciences and Nutrition, Karolinska Institutet, and School of Technology and Health, KTH Royal Institute of Technology, Novum, SE-141 57 Huddinge, Trillo-Muyo, Sergio, Ridley, Caroline, Thornton, David J., Wine, Jeffrey J., Hebert, Hans, Klymiuk, Nikolai, and Hansson, Gunnar C., E-mail: gunnar.hansson@medkem.gu.se. The normal trachea is cleaned by MUC5B mucin bundles from the submucosal glands coated with the MUC5AC mucin. United States: N. p., 2017. Web. doi:10.1016/J.BBRC.2017.08.113.
Ermund, Anna, Meiss, Lauren N., Rodriguez-Pineiro, Ana M., Bähr, Andrea, Nilsson, Harriet E., Department of Biosciences and Nutrition, Karolinska Institutet, and School of Technology and Health, KTH Royal Institute of Technology, Novum, SE-141 57 Huddinge, Trillo-Muyo, Sergio, Ridley, Caroline, Thornton, David J., Wine, Jeffrey J., Hebert, Hans, Klymiuk, Nikolai, & Hansson, Gunnar C., E-mail: gunnar.hansson@medkem.gu.se. The normal trachea is cleaned by MUC5B mucin bundles from the submucosal glands coated with the MUC5AC mucin. United States. doi:10.1016/J.BBRC.2017.08.113.
Ermund, Anna, Meiss, Lauren N., Rodriguez-Pineiro, Ana M., Bähr, Andrea, Nilsson, Harriet E., Department of Biosciences and Nutrition, Karolinska Institutet, and School of Technology and Health, KTH Royal Institute of Technology, Novum, SE-141 57 Huddinge, Trillo-Muyo, Sergio, Ridley, Caroline, Thornton, David J., Wine, Jeffrey J., Hebert, Hans, Klymiuk, Nikolai, and Hansson, Gunnar C., E-mail: gunnar.hansson@medkem.gu.se. Sat . "The normal trachea is cleaned by MUC5B mucin bundles from the submucosal glands coated with the MUC5AC mucin". United States. doi:10.1016/J.BBRC.2017.08.113.
@article{osti_22719109,
title = {The normal trachea is cleaned by MUC5B mucin bundles from the submucosal glands coated with the MUC5AC mucin},
author = {Ermund, Anna and Meiss, Lauren N. and Rodriguez-Pineiro, Ana M. and Bähr, Andrea and Nilsson, Harriet E. and Department of Biosciences and Nutrition, Karolinska Institutet, and School of Technology and Health, KTH Royal Institute of Technology, Novum, SE-141 57 Huddinge and Trillo-Muyo, Sergio and Ridley, Caroline and Thornton, David J. and Wine, Jeffrey J. and Hebert, Hans and Klymiuk, Nikolai and Hansson, Gunnar C., E-mail: gunnar.hansson@medkem.gu.se},
abstractNote = {To understand the mucociliary clearance system, mucins were visualized by light, confocal and electron microscopy, and mucus was stained by Alcian blue and tracked by video microscopy on tracheal explants of newborn piglets. We observed long linear mucus bundles that appeared at the submucosal gland openings and were transported cephalically. The mucus bundles were shown by mass spectrometry and immunostaining to have a core made of MUC5B mucin and were coated with MUC5AC mucin produced by surface goblet cells. The transport speed of the bundles was slower than the airway surface liquid flow. We suggest that the goblet cell MUC5AC mucin anchors the mucus bundles and thus controls their transport. Normal clearance of the respiratory tree of pigs and humans, both rich in submucosal glands, is performed by thick and long mucus bundles. - Highlights: • Submucosal glands in the piglet trachea form bundles of MUC5B mucin. • The mucus bundles are coated with MUC5AC mucin produced by surface goblet cells. • The mucus bundles are transported 10-times slower than the airway surface liquid. • The surface goblet cells are suggested to control the mucus bundle movement.},
doi = {10.1016/J.BBRC.2017.08.113},
journal = {Biochemical and Biophysical Research Communications},
issn = {0006-291X},
number = 3,
volume = 492,
place = {United States},
year = {2017},
month = {10}
}