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Title: Evaluation of human platelet lysate and dimethyl sulfoxide as cryoprotectants for the cryopreservation of human adipose-derived stem cells

Abstract

Cryopreservation provides an effective technique to maintain the functional properties of human adipose-derived stem cells (ASCs). Dimethylsulfoxide (DMSO) and fetal bovine serum (FBS) are frequently used as cryoprotectants for this purpose. However, the use of DMSO can result in adverse effects and toxic reactions and FBS can introduce risks of viral, prion, zoonose contaminations and evoke immune responses after injection. It is therefore crucial to reduce DMSO concentrations and use serum-free solution in the cryopreservation process. Human platelet lysate (PL) is a promising candidate for use as an alternative to DMSO and FBS. Therefore, in this study, with an aim to identify a cryoprotective agent for ASC cryopreservation, we determined the viability, proliferation potential, phenotype, and differentiation potential of fresh ASCs and ASCs cryopreserved using different combinations of three cryoprotective agents: fetal bovine serum (FBS), dimethylsulfoxide (DMSO), and human platelet lysate (PL). The viability of the ASCs cryopreserved with 90% FBS and 10% DMSO, 95% FBS and 5% DMSO, and 97% PL and 3% DMSO was >80%, and the proliferation potentials, cell phenotypes, and differentiation potentials of these groups were similar to those of fresh ASCs. Together, our findings suggest that a combination of 97% PL and 3% DMSO ismore » an ideal cryoprotective agent for the efficient cryopreservation of human ASCs. - Highlights: • 95% FBS with 5% DMSO can cryopreserve ASCs efficiently. • 97% PL with 3% DMSO can cryopreserve ASCs efficiently. • PL can replace FBS as an ideal cryoprotective agent to cryopreserve ASCs. • PL can preserve ASC viability, proliferation, phenotype, and differentiation.« less

Authors:
;  [1];  [1];  [2]
  1. Research Center of Plastic Surgery Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing (China)
  2. Maxillo-facial Surgery Center of Plastic Surgery Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing (China)
Publication Date:
OSTI Identifier:
22719071
Resource Type:
Journal Article
Journal Name:
Biochemical and Biophysical Research Communications
Additional Journal Information:
Journal Volume: 491; Journal Issue: 1; Other Information: Copyright (c) 2017 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; CATTLE; CELL PROLIFERATION; DMSO; FLUORESCEIN; INJECTION; ISOTHIOCYANATES; PHENOTYPE; PVP; STEM CELLS; US FDA

Citation Formats

Wang, Chuan, Xiao, Ran, Cao, Yi-Lin, and Yin, Hong-Yu. Evaluation of human platelet lysate and dimethyl sulfoxide as cryoprotectants for the cryopreservation of human adipose-derived stem cells. United States: N. p., 2017. Web. doi:10.1016/J.BBRC.2017.07.076.
Wang, Chuan, Xiao, Ran, Cao, Yi-Lin, & Yin, Hong-Yu. Evaluation of human platelet lysate and dimethyl sulfoxide as cryoprotectants for the cryopreservation of human adipose-derived stem cells. United States. doi:10.1016/J.BBRC.2017.07.076.
Wang, Chuan, Xiao, Ran, Cao, Yi-Lin, and Yin, Hong-Yu. Sat . "Evaluation of human platelet lysate and dimethyl sulfoxide as cryoprotectants for the cryopreservation of human adipose-derived stem cells". United States. doi:10.1016/J.BBRC.2017.07.076.
@article{osti_22719071,
title = {Evaluation of human platelet lysate and dimethyl sulfoxide as cryoprotectants for the cryopreservation of human adipose-derived stem cells},
author = {Wang, Chuan and Xiao, Ran and Cao, Yi-Lin and Yin, Hong-Yu},
abstractNote = {Cryopreservation provides an effective technique to maintain the functional properties of human adipose-derived stem cells (ASCs). Dimethylsulfoxide (DMSO) and fetal bovine serum (FBS) are frequently used as cryoprotectants for this purpose. However, the use of DMSO can result in adverse effects and toxic reactions and FBS can introduce risks of viral, prion, zoonose contaminations and evoke immune responses after injection. It is therefore crucial to reduce DMSO concentrations and use serum-free solution in the cryopreservation process. Human platelet lysate (PL) is a promising candidate for use as an alternative to DMSO and FBS. Therefore, in this study, with an aim to identify a cryoprotective agent for ASC cryopreservation, we determined the viability, proliferation potential, phenotype, and differentiation potential of fresh ASCs and ASCs cryopreserved using different combinations of three cryoprotective agents: fetal bovine serum (FBS), dimethylsulfoxide (DMSO), and human platelet lysate (PL). The viability of the ASCs cryopreserved with 90% FBS and 10% DMSO, 95% FBS and 5% DMSO, and 97% PL and 3% DMSO was >80%, and the proliferation potentials, cell phenotypes, and differentiation potentials of these groups were similar to those of fresh ASCs. Together, our findings suggest that a combination of 97% PL and 3% DMSO is an ideal cryoprotective agent for the efficient cryopreservation of human ASCs. - Highlights: • 95% FBS with 5% DMSO can cryopreserve ASCs efficiently. • 97% PL with 3% DMSO can cryopreserve ASCs efficiently. • PL can replace FBS as an ideal cryoprotective agent to cryopreserve ASCs. • PL can preserve ASC viability, proliferation, phenotype, and differentiation.},
doi = {10.1016/J.BBRC.2017.07.076},
journal = {Biochemical and Biophysical Research Communications},
issn = {0006-291X},
number = 1,
volume = 491,
place = {United States},
year = {2017},
month = {9}
}