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Title: lncRNA-Map2k4 sequesters miR-199a to promote FGF1 expression and spinal cord neuron growth

Abstract

Spinal cord injury (SCI) is a common critical illness in clinical practice. SCI prevention, treatment and rehabilitation have become important topics in today's medical profession. Studies have shown that long noncoding RNAs (lncRNAs) also play an important role in the pathology of SCI. The biology software analysis identified miR-199a binding sites in the lncRNA-Map2k4 and FGF1 sequences, which were confirmed by the subsequent dual luciferase reporter assay. When lncRNA-Map2k4 expression was down-regulated by siRNA, miR-199a expression in neurons was up-regulated and FGF1 expression was down-regulated. In turn, miR-199a up-regulation inhibited lncRNA-Map2k4 and FGF1 expression. But when lncRNA-Map2k4-m (a lncRNA-Map2k4 overexpression vector with mutated miR-199a binding sites) was co-transfected into neuronal cells with miR-199a mimics, lncRNA-Map2k4-m over-expression did not block the inhibition of FGF1 expression by miR-199a. Moreover, lncRNA-Map2k4 and FGF1 promoted the proliferation and inhibited the apoptosis of neuronal cells, whereas miR-199a down-regulated the aforementioned functions of lncRNA-Map2k4 and FGF1; however, lncRNA-Map2k4-m could not block the inhibitory action of miR-199a on proliferation. Thus, lncRNA-Map2k4 regulates neuronal proliferation and apoptosis through a miR-199a/FGF1 pathway. This finding provides more evidence for the role of lncRNAs in SCI. - Highlights: • lncRNA-Map2k4 is the target gene of miR-199a, and its down-regulation promotes miR-199amore » expression in neurons. • miR-199a targeted regulation of FGF1 expression in neurons. • Knockdown of lncRNA-Map2k4 inhibited the FGF1 expression by up-regulating miR-199a. • lncRNA-Map2k4 regulates neuron proliferation and apoptosis through an miR-199a/FGF1 pathway.« less

Authors:
Publication Date:
OSTI Identifier:
22719055
Resource Type:
Journal Article
Journal Name:
Biochemical and Biophysical Research Communications
Additional Journal Information:
Journal Volume: 490; Journal Issue: 3; Other Information: Copyright (c) 2017 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; APOPTOSIS; INHIBITION; INJURIES; LUCIFERASE; NERVE CELLS; PATHOLOGY; PLANT GROWTH; RNA; SPINAL CORD

Citation Formats

Lv, Hao-ran. lncRNA-Map2k4 sequesters miR-199a to promote FGF1 expression and spinal cord neuron growth. United States: N. p., 2017. Web. doi:10.1016/J.BBRC.2017.06.145.
Lv, Hao-ran. lncRNA-Map2k4 sequesters miR-199a to promote FGF1 expression and spinal cord neuron growth. United States. doi:10.1016/J.BBRC.2017.06.145.
Lv, Hao-ran. Sat . "lncRNA-Map2k4 sequesters miR-199a to promote FGF1 expression and spinal cord neuron growth". United States. doi:10.1016/J.BBRC.2017.06.145.
@article{osti_22719055,
title = {lncRNA-Map2k4 sequesters miR-199a to promote FGF1 expression and spinal cord neuron growth},
author = {Lv, Hao-ran},
abstractNote = {Spinal cord injury (SCI) is a common critical illness in clinical practice. SCI prevention, treatment and rehabilitation have become important topics in today's medical profession. Studies have shown that long noncoding RNAs (lncRNAs) also play an important role in the pathology of SCI. The biology software analysis identified miR-199a binding sites in the lncRNA-Map2k4 and FGF1 sequences, which were confirmed by the subsequent dual luciferase reporter assay. When lncRNA-Map2k4 expression was down-regulated by siRNA, miR-199a expression in neurons was up-regulated and FGF1 expression was down-regulated. In turn, miR-199a up-regulation inhibited lncRNA-Map2k4 and FGF1 expression. But when lncRNA-Map2k4-m (a lncRNA-Map2k4 overexpression vector with mutated miR-199a binding sites) was co-transfected into neuronal cells with miR-199a mimics, lncRNA-Map2k4-m over-expression did not block the inhibition of FGF1 expression by miR-199a. Moreover, lncRNA-Map2k4 and FGF1 promoted the proliferation and inhibited the apoptosis of neuronal cells, whereas miR-199a down-regulated the aforementioned functions of lncRNA-Map2k4 and FGF1; however, lncRNA-Map2k4-m could not block the inhibitory action of miR-199a on proliferation. Thus, lncRNA-Map2k4 regulates neuronal proliferation and apoptosis through a miR-199a/FGF1 pathway. This finding provides more evidence for the role of lncRNAs in SCI. - Highlights: • lncRNA-Map2k4 is the target gene of miR-199a, and its down-regulation promotes miR-199a expression in neurons. • miR-199a targeted regulation of FGF1 expression in neurons. • Knockdown of lncRNA-Map2k4 inhibited the FGF1 expression by up-regulating miR-199a. • lncRNA-Map2k4 regulates neuron proliferation and apoptosis through an miR-199a/FGF1 pathway.},
doi = {10.1016/J.BBRC.2017.06.145},
journal = {Biochemical and Biophysical Research Communications},
issn = {0006-291X},
number = 3,
volume = 490,
place = {United States},
year = {2017},
month = {8}
}