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Title: Cellular localization of ATBF1 protein and its functional implication in breast epithelial cells

Abstract

ATBF1, a large transcription factor, was normally localized in nuclei, and its mislocalization to cytoplasm was reported in multiple cancers. However, localization of ATBF1 in breast epithelial cells and its potential functions were unknown. Here, we investigated ATBF1 localization via immunofluorescence staining in different kinds of breast epithelial cells. In MCF10A cells and normal mice mammary gland tissues, ATBF1 was mainly localized in nuclei. Knockdown of ATBF1 expression in MCF10A cells by siRNA promoted cell proliferation. Moreover, ATBF1 was co-localized with chromosome during mitosis, indicating its potential function in mitosis. In an estrogen receptor (ER)-positive breast cancer cell line (MCF7), estrogen induced ATBF1 translocation from cytoplasm to nuclei in an ER dependent pathway. In ER-negative cells (Hs578T and MDA-MB-231), ATBF1 was co-localized with GM130 in cytoplasm, indicating ATBF1 localization was associated with protein modification in golgi body. The results were beneficial for intensive investigation of ATBF1's function with different cellular localization in breast cancer. - Highlights: • Cellular localization of ATBF1 is correlated with its function in breast cancer. • Nuclear ATBF1 was co-localized with chromosome during mitosis normally. • Estrogen induced translocation of cytoplasmic ATBF1 to nuclei in MCF7. • Translocation of ATBF1 in ER{sup +} breast cancer cellsmore » was ER dependent. • Cytoplasmic ATBF1 was co-localized with golgi body in ER{sup −} breast cancer cells.« less

Authors:
 [1];  [2]; ;  [1];  [1]
  1. Zhejiang Key Laboratory of Pathophysiology, Medical School, Ningbo University, Ningbo, Zhejiang 315211 (China)
  2. (China)
Publication Date:
OSTI Identifier:
22719039
Resource Type:
Journal Article
Journal Name:
Biochemical and Biophysical Research Communications
Additional Journal Information:
Journal Volume: 490; Journal Issue: 2; Other Information: Copyright (c) 2017 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ANIMAL TISSUES; CELL PROLIFERATION; CHROMOSOMES; CYTOPLASM; ESTROGENS; GOLGI COMPLEXES; MAMMARY GLANDS; MICE; MITOSIS; NEOPLASMS; RECEPTORS; TRANSCRIPTION; TRANSCRIPTION FACTORS; TRANSLOCATION

Citation Formats

Li, Mei, Ningbo Institute of Medical Sciences, Ningbo, Zhejiang 315020, Zhang, Chi, Zhong, Yi, and Zhao, Jiyuan. Cellular localization of ATBF1 protein and its functional implication in breast epithelial cells. United States: N. p., 2017. Web. doi:10.1016/J.BBRC.2017.06.068.
Li, Mei, Ningbo Institute of Medical Sciences, Ningbo, Zhejiang 315020, Zhang, Chi, Zhong, Yi, & Zhao, Jiyuan. Cellular localization of ATBF1 protein and its functional implication in breast epithelial cells. United States. doi:10.1016/J.BBRC.2017.06.068.
Li, Mei, Ningbo Institute of Medical Sciences, Ningbo, Zhejiang 315020, Zhang, Chi, Zhong, Yi, and Zhao, Jiyuan. Sat . "Cellular localization of ATBF1 protein and its functional implication in breast epithelial cells". United States. doi:10.1016/J.BBRC.2017.06.068.
@article{osti_22719039,
title = {Cellular localization of ATBF1 protein and its functional implication in breast epithelial cells},
author = {Li, Mei and Ningbo Institute of Medical Sciences, Ningbo, Zhejiang 315020 and Zhang, Chi and Zhong, Yi and Zhao, Jiyuan},
abstractNote = {ATBF1, a large transcription factor, was normally localized in nuclei, and its mislocalization to cytoplasm was reported in multiple cancers. However, localization of ATBF1 in breast epithelial cells and its potential functions were unknown. Here, we investigated ATBF1 localization via immunofluorescence staining in different kinds of breast epithelial cells. In MCF10A cells and normal mice mammary gland tissues, ATBF1 was mainly localized in nuclei. Knockdown of ATBF1 expression in MCF10A cells by siRNA promoted cell proliferation. Moreover, ATBF1 was co-localized with chromosome during mitosis, indicating its potential function in mitosis. In an estrogen receptor (ER)-positive breast cancer cell line (MCF7), estrogen induced ATBF1 translocation from cytoplasm to nuclei in an ER dependent pathway. In ER-negative cells (Hs578T and MDA-MB-231), ATBF1 was co-localized with GM130 in cytoplasm, indicating ATBF1 localization was associated with protein modification in golgi body. The results were beneficial for intensive investigation of ATBF1's function with different cellular localization in breast cancer. - Highlights: • Cellular localization of ATBF1 is correlated with its function in breast cancer. • Nuclear ATBF1 was co-localized with chromosome during mitosis normally. • Estrogen induced translocation of cytoplasmic ATBF1 to nuclei in MCF7. • Translocation of ATBF1 in ER{sup +} breast cancer cells was ER dependent. • Cytoplasmic ATBF1 was co-localized with golgi body in ER{sup −} breast cancer cells.},
doi = {10.1016/J.BBRC.2017.06.068},
journal = {Biochemical and Biophysical Research Communications},
issn = {0006-291X},
number = 2,
volume = 490,
place = {United States},
year = {2017},
month = {8}
}