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Title: Taxifolin enhances osteogenic differentiation of human bone marrow mesenchymal stem cells partially via NF-κB pathway

Abstract

Taxifolin, a flavonoid compound, has been reported to stimulate osteogenic differentiation in osteoblasts. The present study investigated whether taxifolin affects the osteogenic differentiation of human bone marrow mesenchymal stem cells (hBMSCs) and the molecular mechanisms involved. The proliferation and osteogenic differentiation of hBMSCs in the presence of taxifolin were examined by CCK-8 assay, alkaline phosphatase (ALP) activity, ALP staining and Alizarin red staining. The expression of osteogenic differentiation markers were detected by real-time quantitative PCR (RT-PCR) analysis and western blot assay. The activation of potential related pathways was examined by luciferase reporter assay, immunofluorescence and western blot analysis. Taxifolin treatment increased osteogenic differentiation of hBMSCs without cytotoxicity. Luciferase reporter assay showed that taxifolin could not activate estrogen receptor pathway, but inhibit TNF-α-induced NF-κB signaling pathway activation in osteogenic induction condition. Moreover, the nucleus translocation of NF-κB under TNF-α treatment was inhibited by taxifolin treatment. The taxifolin-induced osteogenic differentiation effects of hBMSCs were abolished by TNF-α treatment. In conclusion, our results suggested that taxifolin could promote osteogenesis of hBMSCs, partially through antagonism of NF-κB signaling pathway.

Authors:
 [1];  [1]; ;  [2]; ;  [1]
  1. Department of Spine Surgery, Xiangya Hospital, Central South University (China)
  2. The State Key Laboratory of Medical Genetics, School of Life Sciences, Central South University (China)
Publication Date:
OSTI Identifier:
22719027
Resource Type:
Journal Article
Journal Name:
Biochemical and Biophysical Research Communications
Additional Journal Information:
Journal Volume: 490; Journal Issue: 1; Other Information: Copyright (c) 2017 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ALIZARIN; ALKALINE PHOSPHATASE; BONE MARROW; CONNECTIVE TISSUE CELLS; DMSO; ESTROGENS; FLAVONOIDS; LUCIFERASE; MITOGENS; PHOSPHOTRANSFERASES; POLYMERASE CHAIN REACTION; RECEPTORS; SKELETON; STEM CELLS; TOXICITY; TRANSCRIPTION; TRANSCRIPTION FACTORS; TRANSLOCATION

Citation Formats

Wang, Yun-Jia, Zhang, Hong-Qi, Han, Hai-Long, Zou, Yong-Yi, Gao, Qi-Le, and Yang, Guan-Teng. Taxifolin enhances osteogenic differentiation of human bone marrow mesenchymal stem cells partially via NF-κB pathway. United States: N. p., 2017. Web. doi:10.1016/J.BBRC.2017.06.002.
Wang, Yun-Jia, Zhang, Hong-Qi, Han, Hai-Long, Zou, Yong-Yi, Gao, Qi-Le, & Yang, Guan-Teng. Taxifolin enhances osteogenic differentiation of human bone marrow mesenchymal stem cells partially via NF-κB pathway. United States. doi:10.1016/J.BBRC.2017.06.002.
Wang, Yun-Jia, Zhang, Hong-Qi, Han, Hai-Long, Zou, Yong-Yi, Gao, Qi-Le, and Yang, Guan-Teng. Sat . "Taxifolin enhances osteogenic differentiation of human bone marrow mesenchymal stem cells partially via NF-κB pathway". United States. doi:10.1016/J.BBRC.2017.06.002.
@article{osti_22719027,
title = {Taxifolin enhances osteogenic differentiation of human bone marrow mesenchymal stem cells partially via NF-κB pathway},
author = {Wang, Yun-Jia and Zhang, Hong-Qi and Han, Hai-Long and Zou, Yong-Yi and Gao, Qi-Le and Yang, Guan-Teng},
abstractNote = {Taxifolin, a flavonoid compound, has been reported to stimulate osteogenic differentiation in osteoblasts. The present study investigated whether taxifolin affects the osteogenic differentiation of human bone marrow mesenchymal stem cells (hBMSCs) and the molecular mechanisms involved. The proliferation and osteogenic differentiation of hBMSCs in the presence of taxifolin were examined by CCK-8 assay, alkaline phosphatase (ALP) activity, ALP staining and Alizarin red staining. The expression of osteogenic differentiation markers were detected by real-time quantitative PCR (RT-PCR) analysis and western blot assay. The activation of potential related pathways was examined by luciferase reporter assay, immunofluorescence and western blot analysis. Taxifolin treatment increased osteogenic differentiation of hBMSCs without cytotoxicity. Luciferase reporter assay showed that taxifolin could not activate estrogen receptor pathway, but inhibit TNF-α-induced NF-κB signaling pathway activation in osteogenic induction condition. Moreover, the nucleus translocation of NF-κB under TNF-α treatment was inhibited by taxifolin treatment. The taxifolin-induced osteogenic differentiation effects of hBMSCs were abolished by TNF-α treatment. In conclusion, our results suggested that taxifolin could promote osteogenesis of hBMSCs, partially through antagonism of NF-κB signaling pathway.},
doi = {10.1016/J.BBRC.2017.06.002},
journal = {Biochemical and Biophysical Research Communications},
issn = {0006-291X},
number = 1,
volume = 490,
place = {United States},
year = {2017},
month = {8}
}