skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: The changes in glucose metabolism and cell proliferation in the kidneys of polycystic kidney disease mini-pig models

Abstract

The pathogenic mechanism of polycystic kidney disease (PKD) is unclear. Abnormal glucose metabolism is maybe involved in hyper-proliferation of renal cyst epithelial cells. Mini-pigs are more similar to humans than rodents and therefore, are an ideal large animal model. In this study, for the first time, we systematically investigated the changes in glucose metabolism and cell proliferation signaling pathways in the kidney tissues of chronic progressive PKD mini-pig models created by knock-outing PKD1gene. The results showed that in the kidneys of PKD mini-pigs, the glycolysis is increased and the expressions of key oxidative phosphorylation enzymes Complexes I and IV significantly decreased. The activities of mitochondrial respiration chain Complexes I and IV significantly decreased; the phosphorylation level of key metabolism-modulating molecule AMP-activated protein kinase (AMPK) significantly decreased; and the mammalian target of rapamycin (mTOR) and extracellular signal-regulated kinase (ERK) signaling pathway are activated obviously. This study showed that in the kidneys of PKD mini-pigs, the level of glycolysis significantly increased, oxidative phosphorylation significantly decreased, and cell proliferation signaling pathways significantly activated, suggesting that metabolic changes in PKD may result in the occurrence and development of PKD through the activation of proliferation signaling pathways. - Highlights: • Mini-pigs are more similar tomore » humans than rodents and are an ideal animal model. • The study was first performed in chronic model created by knock-outing PKD1gene. • We investigated the changes in glucose metabolism and cell proliferation. • The glycolysis was significantly increased in the kidneys of PKD mini-pigs. • The mTOR and ERK signaling pathway are activated obviously in PKD mini-pigs.« less

Authors:
 [1]; ; ; ; ;  [1];  [1];  [1]
  1. Department of Nephrology, Chinese PLA General Hospital, Chinese PLA Institute of Nephrology, State Key Laboratory of Kidney Diseases, National Clinical Research Center for Kidney Diseases, Beijing Key Laboratory of Kidney Diseases, Beijing 100853 (China)
Publication Date:
OSTI Identifier:
22719006
Resource Type:
Journal Article
Journal Name:
Biochemical and Biophysical Research Communications
Additional Journal Information:
Journal Volume: 488; Journal Issue: 2; Other Information: Copyright (c) 2017 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; AMP; ANIMAL TISSUES; CELL PROLIFERATION; CYSTS; DISEASES; GLUCOSE; GLYCOLYSIS; KIDNEYS; MITOCHONDRIA; OXIDATION; PHOSPHORYLATION; PHOSPHOTRANSFERASES; RODENTS; SWINE

Citation Formats

Lian, Xiaoying, Beijing Luhe Hospital Capital Medical University, Beijing 101100, Zhao, Jing, Wu, Xiaoyuan, Zhang, Yingjie, Li, Qinggang, Lin, Shupeng, Bai, Xue-Yuan, and Chen, Xiangmei. The changes in glucose metabolism and cell proliferation in the kidneys of polycystic kidney disease mini-pig models. United States: N. p., 2017. Web. doi:10.1016/J.BBRC.2017.05.060.
Lian, Xiaoying, Beijing Luhe Hospital Capital Medical University, Beijing 101100, Zhao, Jing, Wu, Xiaoyuan, Zhang, Yingjie, Li, Qinggang, Lin, Shupeng, Bai, Xue-Yuan, & Chen, Xiangmei. The changes in glucose metabolism and cell proliferation in the kidneys of polycystic kidney disease mini-pig models. United States. doi:10.1016/J.BBRC.2017.05.060.
Lian, Xiaoying, Beijing Luhe Hospital Capital Medical University, Beijing 101100, Zhao, Jing, Wu, Xiaoyuan, Zhang, Yingjie, Li, Qinggang, Lin, Shupeng, Bai, Xue-Yuan, and Chen, Xiangmei. Sat . "The changes in glucose metabolism and cell proliferation in the kidneys of polycystic kidney disease mini-pig models". United States. doi:10.1016/J.BBRC.2017.05.060.
@article{osti_22719006,
title = {The changes in glucose metabolism and cell proliferation in the kidneys of polycystic kidney disease mini-pig models},
author = {Lian, Xiaoying and Beijing Luhe Hospital Capital Medical University, Beijing 101100 and Zhao, Jing and Wu, Xiaoyuan and Zhang, Yingjie and Li, Qinggang and Lin, Shupeng and Bai, Xue-Yuan and Chen, Xiangmei},
abstractNote = {The pathogenic mechanism of polycystic kidney disease (PKD) is unclear. Abnormal glucose metabolism is maybe involved in hyper-proliferation of renal cyst epithelial cells. Mini-pigs are more similar to humans than rodents and therefore, are an ideal large animal model. In this study, for the first time, we systematically investigated the changes in glucose metabolism and cell proliferation signaling pathways in the kidney tissues of chronic progressive PKD mini-pig models created by knock-outing PKD1gene. The results showed that in the kidneys of PKD mini-pigs, the glycolysis is increased and the expressions of key oxidative phosphorylation enzymes Complexes I and IV significantly decreased. The activities of mitochondrial respiration chain Complexes I and IV significantly decreased; the phosphorylation level of key metabolism-modulating molecule AMP-activated protein kinase (AMPK) significantly decreased; and the mammalian target of rapamycin (mTOR) and extracellular signal-regulated kinase (ERK) signaling pathway are activated obviously. This study showed that in the kidneys of PKD mini-pigs, the level of glycolysis significantly increased, oxidative phosphorylation significantly decreased, and cell proliferation signaling pathways significantly activated, suggesting that metabolic changes in PKD may result in the occurrence and development of PKD through the activation of proliferation signaling pathways. - Highlights: • Mini-pigs are more similar to humans than rodents and are an ideal animal model. • The study was first performed in chronic model created by knock-outing PKD1gene. • We investigated the changes in glucose metabolism and cell proliferation. • The glycolysis was significantly increased in the kidneys of PKD mini-pigs. • The mTOR and ERK signaling pathway are activated obviously in PKD mini-pigs.},
doi = {10.1016/J.BBRC.2017.05.060},
journal = {Biochemical and Biophysical Research Communications},
issn = {0006-291X},
number = 2,
volume = 488,
place = {United States},
year = {2017},
month = {6}
}