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Title: The Arabidopsis endoplasmic reticulum associated degradation pathways are involved in the regulation of heat stress response

Abstract

Abstracts: The Cytosolic Protein Response (CPR) in the cytosol and the Unfolded Protein Response (UPR) and ER-associated degradation (ERAD) in the endoplasmic reticulum are major pathways of the cellular proteostasis network. However, despite years of effort, how these protein quality control systems coordinated in vivo remains largely unknown, particularly in plants. In this study, the roles of two evolutionarily conserved ERAD pathways (DOA10 and HRD1) in heat stress response were investigated through reverse genetic approaches in Arabidopsis. Phenotypic analysis of the mutants showed that the two ERAD pathways additively play negative roles in heat tolerance, which was demonstrated by higher survival rate and lower electrolyte leakage in the loss of function mutants compared to the wild type plants. Importantly, gene expression analysis revealed that the mutant plants showed elevated transcriptional regulation of several downstream genes, including those encoding CPR and UPR marker genes, under both basal and heat stress conditions. Finally, multiple components of ERAD genes exhibited rapid response to increasing temperature. Taken together, our data not only unravels key insights into the crosstalk between different protein quality control processes, but also provides candidate genes to genetically improve plant heat tolerance in the future. - Highlights: • ERAD pathways cooperatively regulatemore » plant thermotolerance. • ERAD pathways cooperatively regulate UPR and CPR. • ERAD components gene expression are upregulated by heat stress.« less

Authors:
 [1];  [1];  [1]
  1. Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074 (China)
Publication Date:
OSTI Identifier:
22697033
Resource Type:
Journal Article
Journal Name:
Biochemical and Biophysical Research Communications
Additional Journal Information:
Journal Volume: 487; Journal Issue: 2; Other Information: Copyright (c) 2017 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ARABIDOPSIS; CONTROL SYSTEMS; ENDOPLASMIC RETICULUM; FIRST AID; GENES; HEAT STRESS; MUTANTS; MUTATIONS; QUALITY CONTROL; REGULATIONS

Citation Formats

Li, Lin-Mao, University of Chinese Academy of Sciences, Beijing, Lü, Shi-You, Sino-Africa Joint Research Center, Chinese Academy of Sciences, Wuhan 430074, Li, Rong-Jun, and Sino-Africa Joint Research Center, Chinese Academy of Sciences, Wuhan 430074. The Arabidopsis endoplasmic reticulum associated degradation pathways are involved in the regulation of heat stress response. United States: N. p., 2017. Web. doi:10.1016/J.BBRC.2017.04.066.
Li, Lin-Mao, University of Chinese Academy of Sciences, Beijing, Lü, Shi-You, Sino-Africa Joint Research Center, Chinese Academy of Sciences, Wuhan 430074, Li, Rong-Jun, & Sino-Africa Joint Research Center, Chinese Academy of Sciences, Wuhan 430074. The Arabidopsis endoplasmic reticulum associated degradation pathways are involved in the regulation of heat stress response. United States. doi:10.1016/J.BBRC.2017.04.066.
Li, Lin-Mao, University of Chinese Academy of Sciences, Beijing, Lü, Shi-You, Sino-Africa Joint Research Center, Chinese Academy of Sciences, Wuhan 430074, Li, Rong-Jun, and Sino-Africa Joint Research Center, Chinese Academy of Sciences, Wuhan 430074. Sat . "The Arabidopsis endoplasmic reticulum associated degradation pathways are involved in the regulation of heat stress response". United States. doi:10.1016/J.BBRC.2017.04.066.
@article{osti_22697033,
title = {The Arabidopsis endoplasmic reticulum associated degradation pathways are involved in the regulation of heat stress response},
author = {Li, Lin-Mao and University of Chinese Academy of Sciences, Beijing and Lü, Shi-You and Sino-Africa Joint Research Center, Chinese Academy of Sciences, Wuhan 430074 and Li, Rong-Jun and Sino-Africa Joint Research Center, Chinese Academy of Sciences, Wuhan 430074},
abstractNote = {Abstracts: The Cytosolic Protein Response (CPR) in the cytosol and the Unfolded Protein Response (UPR) and ER-associated degradation (ERAD) in the endoplasmic reticulum are major pathways of the cellular proteostasis network. However, despite years of effort, how these protein quality control systems coordinated in vivo remains largely unknown, particularly in plants. In this study, the roles of two evolutionarily conserved ERAD pathways (DOA10 and HRD1) in heat stress response were investigated through reverse genetic approaches in Arabidopsis. Phenotypic analysis of the mutants showed that the two ERAD pathways additively play negative roles in heat tolerance, which was demonstrated by higher survival rate and lower electrolyte leakage in the loss of function mutants compared to the wild type plants. Importantly, gene expression analysis revealed that the mutant plants showed elevated transcriptional regulation of several downstream genes, including those encoding CPR and UPR marker genes, under both basal and heat stress conditions. Finally, multiple components of ERAD genes exhibited rapid response to increasing temperature. Taken together, our data not only unravels key insights into the crosstalk between different protein quality control processes, but also provides candidate genes to genetically improve plant heat tolerance in the future. - Highlights: • ERAD pathways cooperatively regulate plant thermotolerance. • ERAD pathways cooperatively regulate UPR and CPR. • ERAD components gene expression are upregulated by heat stress.},
doi = {10.1016/J.BBRC.2017.04.066},
journal = {Biochemical and Biophysical Research Communications},
issn = {0006-291X},
number = 2,
volume = 487,
place = {United States},
year = {2017},
month = {5}
}