SNHG1 lncRNA negatively regulates miR-199a-3p to enhance CDK7 expression and promote cell proliferation in prostate cancer
- Department of Operation, The Affiliated Hospital of Southwest Medical University, Luzhou (China)
- School of Pubilc Health, Guangdong Pharmaceutical University, Guangzhou (China)
- People's Hospital of Luxian, Luzhou (China)
- Department of Urology, Chengdu Chengfei Hospital, Chengdu (China)
- Department of Urology, The Affiliated Hospital of Southwest Medical University, Luzhou (China)
Long noncoding RNAs (lncRNAs) have been reported to play vital roles in the development of human cancers, but our understandings of most lncRNAs in cancers are still limited. Recently, accumlating evidences have showed that many RNA transcripts could function as competing endogenous RNAs (ceRNAs) by competitively binding common microRNAs. In this study, we demonstrated that a lncRNA, Small Nucleolar RNA Host Gene 1 (SNHG1), as a ceRNA for miR-199a-3p, played a critical role in prostate cancer cell proliferation. We found that SNHG1 was aberrantly up-regulated in prostate carcinoma tissues; while, miR-199a-3p was abnormally down-regulated. The level of SNHG1 in prostate cancer was significantly negatively correlated with that of miR-199a-3p. Our data indicated that SNHG1 could interact with miR-199a-3p and inhibit the activity of miR-199a-3p in prostate cancer cells. In addition, miR-199a-3p could target the 3′ UTR of CDK7 and suppress CDK7 expression. More importantly, SNHG1 increased CDK7 expression by competitively binding miR-199a-3p, and then promoted cell proliferation and cell cycle progression in prostate cancer. Taken together, these findings elucidated a novel mechanism of prostate cancer progression. Thus, SNHG1 might serve as a potential target for prostate cancer therapies. - Highlights: • SNHG1 was up-regulated in prostate cancer. • miR-199a-3p was down-regulated in prostate cancer. • SNHG1 negatively regulated miR-199a-3p activity in prostate cancer. • miR-199a-3p negatively modulated CDK7 expression in prostate cancer. • SNHG1 positively regulated CDK7 expression by targeting miR-199a-3p.
- OSTI ID:
- 22697022
- Journal Information:
- Biochemical and Biophysical Research Communications, Vol. 487, Issue 1; Other Information: Copyright (c) 2017 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
- Country of Publication:
- United States
- Language:
- English
Similar Records
LncRNA ZEB1-AS1/miR-409–3p/ZEB1 feedback loop is involved in the progression of non-small cell lung cancer
lncRNA-Map2k4 sequesters miR-199a to promote FGF1 expression and spinal cord neuron growth