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Title: ALG2 regulates glioblastoma cell proliferation, migration and tumorigenicity

Abstract

Apoptosis-linked gene-2 (ALG-2), also known as programmed cell death 6 (PDCD6), has recently been reported to be aberrantly expressed in various tumors and required for tumor cell viability. The aim of the present study was to investigate whether ALG-2 plays a crucial role in tumor cell proliferation, migration and tumorigenicity. In this study, we examined the expression of PDCD6 in glioblastoma cell lines and found that ALG-2 was generally expressed in glioblastoma cell lines. We also performed an analysis of an online database and found that high expression of ALG-2 was associated with poor prognosis (p = 0.039). We found that over-expression of ALG2 in glioblastoma could inhibit cell proliferation and, conversely, that down-regulation of ALG2 could promote cell proliferation. Further studies showed that over-expression of ALG2 inhibited the migration of tumor cells, whereas down-regulation of ALG2 promoted tumor cell migration. Finally, in vitro and in vivo studies showed that over-expression of ALG2 inhibited the tumorigenic ability of tumor cells, while down-regulation of ALG2 promoted tumor cell tumorigenic ability. In conclusion, ALG2 has a tumor suppressive role in glioblastoma and might be a potential target for the treatment of glioblastoma. - Highlights: • Low ALG2 expression is indicative of poor prognosis in glioblastoma patients.more » • ALG2 is required for cell proliferation in GBM cells. • ALG2 is involved in GBM cell migration. • ALG2 is involved in GBM cell self-renewal and tumorigenesis in vitro and in vivo.« less

Authors:
; ; ; ;  [1];  [2];  [1]
  1. State Key Laboratory of Silkworm Genome Biology, The Institute of Sericulture and Systems Biology, Southwest University, Chongqing 400716 (China)
  2. Department of Pharmaceutical Sciences, Eugene Applebaum College of Pharmacy and Health Sciences, Wayne State University, 259 Mack Avenue, Detroit, MI 48201 (United States)
Publication Date:
OSTI Identifier:
22696984
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 486; Journal Issue: 2; Other Information: Copyright (c) 2017 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; CELL PROLIFERATION; GLIOMAS; IN VIVO; INHIBITION; MIGRATION; TUMOR CELLS

Citation Formats

Zhang, Dunke, Wang, Feng, Pang, Yi, Zhao, Erhu, Zhu, Sunqin, Chen, Fei, and Cui, Hongjuan. ALG2 regulates glioblastoma cell proliferation, migration and tumorigenicity. United States: N. p., 2017. Web. doi:10.1016/J.BBRC.2017.03.032.
Zhang, Dunke, Wang, Feng, Pang, Yi, Zhao, Erhu, Zhu, Sunqin, Chen, Fei, & Cui, Hongjuan. ALG2 regulates glioblastoma cell proliferation, migration and tumorigenicity. United States. doi:10.1016/J.BBRC.2017.03.032.
Zhang, Dunke, Wang, Feng, Pang, Yi, Zhao, Erhu, Zhu, Sunqin, Chen, Fei, and Cui, Hongjuan. Sat . "ALG2 regulates glioblastoma cell proliferation, migration and tumorigenicity". United States. doi:10.1016/J.BBRC.2017.03.032.
@article{osti_22696984,
title = {ALG2 regulates glioblastoma cell proliferation, migration and tumorigenicity},
author = {Zhang, Dunke and Wang, Feng and Pang, Yi and Zhao, Erhu and Zhu, Sunqin and Chen, Fei and Cui, Hongjuan},
abstractNote = {Apoptosis-linked gene-2 (ALG-2), also known as programmed cell death 6 (PDCD6), has recently been reported to be aberrantly expressed in various tumors and required for tumor cell viability. The aim of the present study was to investigate whether ALG-2 plays a crucial role in tumor cell proliferation, migration and tumorigenicity. In this study, we examined the expression of PDCD6 in glioblastoma cell lines and found that ALG-2 was generally expressed in glioblastoma cell lines. We also performed an analysis of an online database and found that high expression of ALG-2 was associated with poor prognosis (p = 0.039). We found that over-expression of ALG2 in glioblastoma could inhibit cell proliferation and, conversely, that down-regulation of ALG2 could promote cell proliferation. Further studies showed that over-expression of ALG2 inhibited the migration of tumor cells, whereas down-regulation of ALG2 promoted tumor cell migration. Finally, in vitro and in vivo studies showed that over-expression of ALG2 inhibited the tumorigenic ability of tumor cells, while down-regulation of ALG2 promoted tumor cell tumorigenic ability. In conclusion, ALG2 has a tumor suppressive role in glioblastoma and might be a potential target for the treatment of glioblastoma. - Highlights: • Low ALG2 expression is indicative of poor prognosis in glioblastoma patients. • ALG2 is required for cell proliferation in GBM cells. • ALG2 is involved in GBM cell migration. • ALG2 is involved in GBM cell self-renewal and tumorigenesis in vitro and in vivo.},
doi = {10.1016/J.BBRC.2017.03.032},
journal = {Biochemical and Biophysical Research Communications},
number = 2,
volume = 486,
place = {United States},
year = {Sat Apr 29 00:00:00 EDT 2017},
month = {Sat Apr 29 00:00:00 EDT 2017}
}