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Title: Production of ABA responses requires both the nuclear and cytoplasmic functional involvement of PYR1

Abstract

Abscisic acid (ABA) enhances stress tolerant responses in plants against unfavorable environmental conditions. In Arabidopsis, ABA promotes interactions between PYR/PYL/RCARs and PP2C, thereby allowing SnRK2s to phosphorylate downstream components required for the regulation of gene expression or for gating ion channels. Because PYR1 is known to localize to nucleus and cytoplasm it is a question whether nuclear or cytoplasmic PYR1 confer different functions to the ABA signaling pathway, as has been previously shown for regulatory proteins. In order to answer this question, transgenic lines expressing nuclear PYR1 were generated in an ABA insensitive mutant background. Enforced nuclear expression of PYR1 was examined by confocal microscopy and western blot analysis. Physiological analyses of the transgenic lines demonstrated that nuclear PYR1 is sufficient to generate ABA responses, such as, the inhibition of seed germination, root growth inhibition, the induction of gene expression, and stomatal closing movement. However, for the full recovery of ABA responses in the mutant background cytoplasmic PYR1 was required. The study suggests both nuclear and cytoplasmic PYR1 participate in the control of ABA signal transduction. - Highlights: • Nuclear and cytoplasmic functions of PYR1 were studied in the mutant which lacked majority of ABA responses. • Nuclear PYR1 reconstitutedmore » partially the ABA responses during seed germination, root growth, and guard cell movement. • Both the nuclear and cytoplasmic functions of PYR1 were required for the full generation of ABA responses.« less

Authors:
;
Publication Date:
OSTI Identifier:
22696868
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 484; Journal Issue: 1; Other Information: Copyright (c) 2017 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ABSCISIC ACID; CYTOPLASM; GENES; INHIBITION; IONS; MUTANTS; MUTATIONS; PLANT GROWTH

Citation Formats

Park, EunJoo, and Kim, Tae-Houn. Production of ABA responses requires both the nuclear and cytoplasmic functional involvement of PYR1. United States: N. p., 2017. Web. doi:10.1016/J.BBRC.2017.01.082.
Park, EunJoo, & Kim, Tae-Houn. Production of ABA responses requires both the nuclear and cytoplasmic functional involvement of PYR1. United States. doi:10.1016/J.BBRC.2017.01.082.
Park, EunJoo, and Kim, Tae-Houn. Sun . "Production of ABA responses requires both the nuclear and cytoplasmic functional involvement of PYR1". United States. doi:10.1016/J.BBRC.2017.01.082.
@article{osti_22696868,
title = {Production of ABA responses requires both the nuclear and cytoplasmic functional involvement of PYR1},
author = {Park, EunJoo and Kim, Tae-Houn},
abstractNote = {Abscisic acid (ABA) enhances stress tolerant responses in plants against unfavorable environmental conditions. In Arabidopsis, ABA promotes interactions between PYR/PYL/RCARs and PP2C, thereby allowing SnRK2s to phosphorylate downstream components required for the regulation of gene expression or for gating ion channels. Because PYR1 is known to localize to nucleus and cytoplasm it is a question whether nuclear or cytoplasmic PYR1 confer different functions to the ABA signaling pathway, as has been previously shown for regulatory proteins. In order to answer this question, transgenic lines expressing nuclear PYR1 were generated in an ABA insensitive mutant background. Enforced nuclear expression of PYR1 was examined by confocal microscopy and western blot analysis. Physiological analyses of the transgenic lines demonstrated that nuclear PYR1 is sufficient to generate ABA responses, such as, the inhibition of seed germination, root growth inhibition, the induction of gene expression, and stomatal closing movement. However, for the full recovery of ABA responses in the mutant background cytoplasmic PYR1 was required. The study suggests both nuclear and cytoplasmic PYR1 participate in the control of ABA signal transduction. - Highlights: • Nuclear and cytoplasmic functions of PYR1 were studied in the mutant which lacked majority of ABA responses. • Nuclear PYR1 reconstituted partially the ABA responses during seed germination, root growth, and guard cell movement. • Both the nuclear and cytoplasmic functions of PYR1 were required for the full generation of ABA responses.},
doi = {10.1016/J.BBRC.2017.01.082},
journal = {Biochemical and Biophysical Research Communications},
number = 1,
volume = 484,
place = {United States},
year = {Sun Feb 26 00:00:00 EST 2017},
month = {Sun Feb 26 00:00:00 EST 2017}
}