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Title: Regulator of G protein signaling 4 is a novel target of GATA-6 transcription factor

Abstract

GATA transcription factors regulate an array of genes important in cell proliferation and differentiation. Here we report the identification of regulator of G protein signaling 4 (RGS4) as a novel target for GATA-6 transcription factor. Although three sites (a, b, c) within the proximal region of rabbit RGS4 promoter for GATA transcription factors were predicted by bioinformatics analysis, only GATA-a site (16 bp from the core TATA box) is essential for RGS4 transcriptional regulation. RT-PCR analysis demonstrated that only GATA-6 was highly expressed in rabbit colonic smooth muscle cells but GATA-4/6 were expressed in cardiac myocytes and GATA-1/2/3 expressed in blood cells. Adenovirus-mediated expression of GATA-6 but not GATA-1 significantly increased the constitutive and IL-1β-induced mRNA expression of the endogenous RGS4 in colonic smooth muscle cells. IL-1β stimulation induced GATA-6 nuclear translocation and increased GATA-6 binding to RGS4 promoter. These data suggest that GATA factor could affect G protein signaling through regulating RGS4 expression, and GATA signaling may develop as a future therapeutic target for RGS4-related diseases. - Highlights: • GATA-6 is highly expressed in colonic smooth muscle cells. • RGS4 is a novel target for GATA-6 transcription factor. • GATA-a response element is essential to regulate the core promotermore » of RGS4. • GATA-6 regulates IL-1β-induced RGS4 upregulation.« less

Authors:
; ;  [1];  [2]; ;  [1];  [2];  [1]
  1. Department of Neuroscience, Temple University Lewis Katz School of Medicine, 3500 N Broad Street, Philadelphia, PA 19140 (United States)
  2. Department of Physiology and Biophysics, Medical College of Virginia Campus, Virginia Commonwealth University, 1101 East Marshall Street, Richmond, VA 23298 (United States)
Publication Date:
OSTI Identifier:
22696859
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 483; Journal Issue: 3; Other Information: Copyright (c) 2016 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; BLOOD CELLS; CELL PROLIFERATION; GENE REGULATION; GTP-ASES; MUSCLES; PROMOTERS; SIGNALS; TRANSCRIPTION FACTORS

Citation Formats

Zhang, Yonggang, Li, Fang, Xiao, Xiao, Deng, Wu, Yin, Chaoran, Zhang, Ting, Murthy, Karnam S., and Hu, Wenhui. Regulator of G protein signaling 4 is a novel target of GATA-6 transcription factor. United States: N. p., 2017. Web. doi:10.1016/J.BBRC.2016.10.024.
Zhang, Yonggang, Li, Fang, Xiao, Xiao, Deng, Wu, Yin, Chaoran, Zhang, Ting, Murthy, Karnam S., & Hu, Wenhui. Regulator of G protein signaling 4 is a novel target of GATA-6 transcription factor. United States. doi:10.1016/J.BBRC.2016.10.024.
Zhang, Yonggang, Li, Fang, Xiao, Xiao, Deng, Wu, Yin, Chaoran, Zhang, Ting, Murthy, Karnam S., and Hu, Wenhui. Sun . "Regulator of G protein signaling 4 is a novel target of GATA-6 transcription factor". United States. doi:10.1016/J.BBRC.2016.10.024.
@article{osti_22696859,
title = {Regulator of G protein signaling 4 is a novel target of GATA-6 transcription factor},
author = {Zhang, Yonggang and Li, Fang and Xiao, Xiao and Deng, Wu and Yin, Chaoran and Zhang, Ting and Murthy, Karnam S. and Hu, Wenhui},
abstractNote = {GATA transcription factors regulate an array of genes important in cell proliferation and differentiation. Here we report the identification of regulator of G protein signaling 4 (RGS4) as a novel target for GATA-6 transcription factor. Although three sites (a, b, c) within the proximal region of rabbit RGS4 promoter for GATA transcription factors were predicted by bioinformatics analysis, only GATA-a site (16 bp from the core TATA box) is essential for RGS4 transcriptional regulation. RT-PCR analysis demonstrated that only GATA-6 was highly expressed in rabbit colonic smooth muscle cells but GATA-4/6 were expressed in cardiac myocytes and GATA-1/2/3 expressed in blood cells. Adenovirus-mediated expression of GATA-6 but not GATA-1 significantly increased the constitutive and IL-1β-induced mRNA expression of the endogenous RGS4 in colonic smooth muscle cells. IL-1β stimulation induced GATA-6 nuclear translocation and increased GATA-6 binding to RGS4 promoter. These data suggest that GATA factor could affect G protein signaling through regulating RGS4 expression, and GATA signaling may develop as a future therapeutic target for RGS4-related diseases. - Highlights: • GATA-6 is highly expressed in colonic smooth muscle cells. • RGS4 is a novel target for GATA-6 transcription factor. • GATA-a response element is essential to regulate the core promoter of RGS4. • GATA-6 regulates IL-1β-induced RGS4 upregulation.},
doi = {10.1016/J.BBRC.2016.10.024},
journal = {Biochemical and Biophysical Research Communications},
number = 3,
volume = 483,
place = {United States},
year = {Sun Feb 12 00:00:00 EST 2017},
month = {Sun Feb 12 00:00:00 EST 2017}
}