Hydroxyl radical generation by dissociation of water molecules during 1.65 MHz frequency ultrasound irradiation under aerobic conditions
- School of Materials and Chemical Technology, Tokyo Institute of Technology, 4259-G1-14, Nagatsuta-cho, Midori-ku, Yokohama 226-8502 (Japan)
- School of Bioscience and Biotechnology, Tokyo Institute of Technology, 2-12-1, Ookayama, Meguro-ku, Tokyo 152-8550 (Japan)
- Showa Pharmaceutical University, 3-3165 Higashi-tamagawagakuen, Machida, Tokyo 194-8543 (Japan)
The dissociation of water molecules by ultrasound irradiation under aerobic conditions was demonstrated experimentally. To be able to detect the dissociation of water molecules, we performed the ultrasound irradiation of {sup 17}O-labelled water (H{sub 2}{sup 17}O) under aerobic conditions. The hydroxyl and hydrogen radicals generated during the ultrasound irradiation process were trapped with 5,5-dimethyl-1-pyrroline-N-oxide (DMPO), and electron spin resonance (ESR) spectroscopy was performed on the DMPO spin adducts. In the ESR spectrum, a 15-line signal attributable to the trapping of the hydroxyl radicals containing {sup 17}O ({sup 17}OH radicals) by DMPO together with a 4-line signal attributable to the trapping of the hydroxyl radicals containing {sup 16}O ({sup 16}OH radicals) by DMPO were observed. The generation of {sup 17}OH radicals indicated that H{sub 2}{sup 17}O was dissociated by the sonolysis process under aerobic conditions. On the other hand, the ESR signal attributable to the trapping of hydrogen radicals by DMPO was not observed, suggesting that hydrogen radicals were not generated during the dissociation of water molecules. - Highlights: • OH radicals was observed in air-saturated water under 1.65 MHz ultrasound. • The OH radicals were originated from both H{sub 2}O and O{sub 2.} • The H{sub 2}O sonolysis was detected by using {sup 17}O-labelled water.
- OSTI ID:
- 22696820
- Journal Information:
- Biochemical and Biophysical Research Communications, Vol. 483, Issue 1; Other Information: Copyright (c) 2017 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
- Country of Publication:
- United States
- Language:
- English
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