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Title: Aspirin increases mitochondrial fatty acid oxidation

Abstract

The metabolic effects of salicylates are poorly understood. This study investigated the effects of aspirin on fatty acid oxidation. Aspirin increased mitochondrial long-chain fatty acid oxidation, but inhibited peroxisomal fatty acid oxidation, in two different cell lines. Aspirin increased mitochondrial protein acetylation and was found to be a stronger acetylating agent in vitro than acetyl-CoA. However, aspirin-induced acetylation did not alter the activity of fatty acid oxidation proteins, and knocking out the mitochondrial deacetylase SIRT3 did not affect the induction of long-chain fatty acid oxidation by aspirin. Aspirin did not change oxidation of medium-chain fatty acids, which can freely traverse the mitochondrial membrane. Together, these data indicate that aspirin does not directly alter mitochondrial matrix fatty acid oxidation enzymes, but most likely exerts its effects at the level of long-chain fatty acid transport into mitochondria. The drive on mitochondrial fatty acid oxidation may be a compensatory response to altered mitochondrial morphology and inhibited electron transport chain function, both of which were observed after 24 h incubation of cells with aspirin. These studies provide insight into the pathophysiology of Reye Syndrome, which is known to be triggered by aspirin ingestion in patients with fatty acid oxidation disorders. - Highlights: • Aspirin increases mitochondrial—butmore » inhibits peroxisomal—fatty acid oxidation. • Aspirin acetylates mitochondrial proteins including fatty acid oxidation enzymes. • SIRT3 does not influence the effect of aspirin on fatty acid oxidation. • Increased fatty acid oxidation is likely due to altered mitochondrial morphology and respiration.« less

Authors:
; ; ; ;  [1];  [2];  [1]
  1. Department of Pediatrics, Children's Hospital of Pittsburgh of UPMC, Pittsburgh, PA 15224 (United States)
  2. Center for Biologic Imaging, Department of Cell Biology, University of Pittsburgh, Pittsburgh, PA 15260 (United States)
Publication Date:
OSTI Identifier:
22696756
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 482; Journal Issue: 2; Other Information: Copyright (c) 2016 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ACETYLATION; ACETYLSALICYLIC ACID; INHIBITION; MITOCHONDRIA; OXIDATION; OXIDOREDUCTASES

Citation Formats

Uppala, Radha, Dudiak, Brianne, Beck, Megan E., Bharathi, Sivakama S., Zhang, Yuxun, Stolz, Donna B., and Goetzman, Eric S., E-mail: Eric.goetzman@chp.edu. Aspirin increases mitochondrial fatty acid oxidation. United States: N. p., 2017. Web. doi:10.1016/J.BBRC.2016.11.066.
Uppala, Radha, Dudiak, Brianne, Beck, Megan E., Bharathi, Sivakama S., Zhang, Yuxun, Stolz, Donna B., & Goetzman, Eric S., E-mail: Eric.goetzman@chp.edu. Aspirin increases mitochondrial fatty acid oxidation. United States. doi:10.1016/J.BBRC.2016.11.066.
Uppala, Radha, Dudiak, Brianne, Beck, Megan E., Bharathi, Sivakama S., Zhang, Yuxun, Stolz, Donna B., and Goetzman, Eric S., E-mail: Eric.goetzman@chp.edu. Sun . "Aspirin increases mitochondrial fatty acid oxidation". United States. doi:10.1016/J.BBRC.2016.11.066.
@article{osti_22696756,
title = {Aspirin increases mitochondrial fatty acid oxidation},
author = {Uppala, Radha and Dudiak, Brianne and Beck, Megan E. and Bharathi, Sivakama S. and Zhang, Yuxun and Stolz, Donna B. and Goetzman, Eric S., E-mail: Eric.goetzman@chp.edu},
abstractNote = {The metabolic effects of salicylates are poorly understood. This study investigated the effects of aspirin on fatty acid oxidation. Aspirin increased mitochondrial long-chain fatty acid oxidation, but inhibited peroxisomal fatty acid oxidation, in two different cell lines. Aspirin increased mitochondrial protein acetylation and was found to be a stronger acetylating agent in vitro than acetyl-CoA. However, aspirin-induced acetylation did not alter the activity of fatty acid oxidation proteins, and knocking out the mitochondrial deacetylase SIRT3 did not affect the induction of long-chain fatty acid oxidation by aspirin. Aspirin did not change oxidation of medium-chain fatty acids, which can freely traverse the mitochondrial membrane. Together, these data indicate that aspirin does not directly alter mitochondrial matrix fatty acid oxidation enzymes, but most likely exerts its effects at the level of long-chain fatty acid transport into mitochondria. The drive on mitochondrial fatty acid oxidation may be a compensatory response to altered mitochondrial morphology and inhibited electron transport chain function, both of which were observed after 24 h incubation of cells with aspirin. These studies provide insight into the pathophysiology of Reye Syndrome, which is known to be triggered by aspirin ingestion in patients with fatty acid oxidation disorders. - Highlights: • Aspirin increases mitochondrial—but inhibits peroxisomal—fatty acid oxidation. • Aspirin acetylates mitochondrial proteins including fatty acid oxidation enzymes. • SIRT3 does not influence the effect of aspirin on fatty acid oxidation. • Increased fatty acid oxidation is likely due to altered mitochondrial morphology and respiration.},
doi = {10.1016/J.BBRC.2016.11.066},
journal = {Biochemical and Biophysical Research Communications},
number = 2,
volume = 482,
place = {United States},
year = {Sun Jan 08 00:00:00 EST 2017},
month = {Sun Jan 08 00:00:00 EST 2017}
}