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Title: Elevated expression of WWP2 in human lung adenocarcinoma and its effect on migration and invasion

Abstract

Lung cancer has been a hot area of research because of its high incidence and mortality. In this study, WWP2, an E3 ubiquitin ligase, is proposed to be an oncoprotein contributing to lung tumorigenesis. We attempted to determine if WWP2 gene expression is correlated with the development of human lung adenocarcinoma. Real-time PCR and western blotting were used to detect the expression of WWP2 in 65 paired lung adenocarcinoma and adjacent normal lung tissues. We found that WWP2 expression was elevated in lung adenocarcinoma tissues and was correlated with the tumor differentiation stage, TNM stage and presence of lymph node metastasis. We performed CCK-8 and colony formation assays and found that down-regulation of WWP2 inhibited proliferation in A549 and SPC-A-1 cells. A wound healing assay and trans-well invasion assays showed that down-regulation of WWP2 inhibited the migration and invasion of lung adenocarcinoma cells. It could be predicted from these data that elevated expression of WWP2 may play a role in facilitating the development of lung adenocarcinoma. - Highlights: • Expression of WWP2 is firstly reported in human lung adenocarcinoma. • Function of WWP2 is firstly explored in lung adenocarcinoma cells.

Authors:
 [1];  [2]; ; ;  [1];  [1]
  1. Department of Respiratory Medicine, The First Affiliated Hospital, Zhengzhou University, Zhengzhou (China)
  2. Department of Ultrasound, People's Hospital of Zhengzhou University, Zhengzhou (China)
Publication Date:
OSTI Identifier:
22696636
Resource Type:
Journal Article
Journal Name:
Biochemical and Biophysical Research Communications
Additional Journal Information:
Journal Volume: 479; Journal Issue: 2; Other Information: Copyright (c) 2016 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ANIMAL TISSUES; CARCINOMAS; COLONY FORMATION; LUNGS; LYMPH NODES; MIGRATION; POLYMERASE CHAIN REACTION

Citation Formats

Yang, Rui, He, Yao, Chen, Shanshan, Lu, Xinhua, Huang, Chun, and Zhang, Guojun. Elevated expression of WWP2 in human lung adenocarcinoma and its effect on migration and invasion. United States: N. p., 2016. Web. doi:10.1016/J.BBRC.2016.07.084.
Yang, Rui, He, Yao, Chen, Shanshan, Lu, Xinhua, Huang, Chun, & Zhang, Guojun. Elevated expression of WWP2 in human lung adenocarcinoma and its effect on migration and invasion. United States. doi:10.1016/J.BBRC.2016.07.084.
Yang, Rui, He, Yao, Chen, Shanshan, Lu, Xinhua, Huang, Chun, and Zhang, Guojun. Fri . "Elevated expression of WWP2 in human lung adenocarcinoma and its effect on migration and invasion". United States. doi:10.1016/J.BBRC.2016.07.084.
@article{osti_22696636,
title = {Elevated expression of WWP2 in human lung adenocarcinoma and its effect on migration and invasion},
author = {Yang, Rui and He, Yao and Chen, Shanshan and Lu, Xinhua and Huang, Chun and Zhang, Guojun},
abstractNote = {Lung cancer has been a hot area of research because of its high incidence and mortality. In this study, WWP2, an E3 ubiquitin ligase, is proposed to be an oncoprotein contributing to lung tumorigenesis. We attempted to determine if WWP2 gene expression is correlated with the development of human lung adenocarcinoma. Real-time PCR and western blotting were used to detect the expression of WWP2 in 65 paired lung adenocarcinoma and adjacent normal lung tissues. We found that WWP2 expression was elevated in lung adenocarcinoma tissues and was correlated with the tumor differentiation stage, TNM stage and presence of lymph node metastasis. We performed CCK-8 and colony formation assays and found that down-regulation of WWP2 inhibited proliferation in A549 and SPC-A-1 cells. A wound healing assay and trans-well invasion assays showed that down-regulation of WWP2 inhibited the migration and invasion of lung adenocarcinoma cells. It could be predicted from these data that elevated expression of WWP2 may play a role in facilitating the development of lung adenocarcinoma. - Highlights: • Expression of WWP2 is firstly reported in human lung adenocarcinoma. • Function of WWP2 is firstly explored in lung adenocarcinoma cells.},
doi = {10.1016/J.BBRC.2016.07.084},
journal = {Biochemical and Biophysical Research Communications},
issn = {0006-291X},
number = 2,
volume = 479,
place = {United States},
year = {2016},
month = {10}
}