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Title: Smoking-related microRNAs and mRNAs in human peripheral blood mononuclear cells

Abstract

Teenager smoking is of great importance in public health. Functional roles of microRNAs have been documented in smoke-induced gene expression changes, but comprehensive mechanisms of microRNA-mRNA regulation and benefits remained poorly understood. We conducted the Teenager Smoking Reduction Trial (TSRT) to investigate the causal association between active smoking reduction and whole-genome microRNA and mRNA expression changes in human peripheral blood mononuclear cells (PBMC). A total of 12 teenagers with a substantial reduction in smoke quantity and a decrease in urine cotinine/creatinine ratio were enrolled in genomic analyses. In Gene Set Enrichment Analysis (GSEA) and Ingenuity Pathway Analysis (IPA), differentially expressed genes altered by smoke reduction were mainly associated with glucocorticoid receptor signaling pathway. The integrative analysis of microRNA and mRNA found eleven differentially expressed microRNAs negatively correlated with predicted target genes. CD83 molecule regulated by miR-4498 in human PBMC, was critical for the canonical pathway of communication between innate and adaptive immune cells. Our data demonstrated that microRNAs could regulate immune responses in human PBMC after habitual smokers quit smoking and support the potential translational value of microRNAs in regulating disease-relevant gene expression caused by tobacco smoke. - Highlights: • We conducted a smoke reduction trial program and investigated themore » causal relationship between smoke and gene regulation. • MicroRNA and mRNA expression changes were examined in human PBMC. • MicroRNAs are important in regulating disease-causal genes after tobacco smoke reduction.« less

Authors:
 [1];  [2];  [3];  [1];  [4]
  1. Institute of Epidemiology and Preventive Medicine, College of Public Health, National Taiwan University, Taipei, Taiwan (China)
  2. Department of Clinical Laboratory Sciences and Medical Biotechnology, College of Medicine, National Taiwan University, Taipei, Taiwan (China)
  3. Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan (China)
  4. School of Medicine, National Taiwan University, Taipei, Taiwan (China)
Publication Date:
OSTI Identifier:
22689236
Resource Type:
Journal Article
Journal Name:
Toxicology and Applied Pharmacology
Additional Journal Information:
Journal Volume: 305; Other Information: Copyright (c) 2016 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0041-008X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; BLOOD; CREATININE; DISEASES; ENRICHMENT; GENE REGULATION; GENES; GLUCOCORTICOIDS; MESSENGER-RNA; MOLECULES; PUBLIC HEALTH; RECEPTORS; REDUCTION; TOBACCO; URINE

Citation Formats

Su, Ming-Wei, Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan, Yu, Sung-Liang, Lin, Wen-Chang, Tsai, Ching-Hui, Chen, Po-Hua, Lee, Yungling Leo, E-mail: leolee@ntu.edu.tw, and Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan. Smoking-related microRNAs and mRNAs in human peripheral blood mononuclear cells. United States: N. p., 2016. Web. doi:10.1016/J.TAAP.2016.06.020.
Su, Ming-Wei, Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan, Yu, Sung-Liang, Lin, Wen-Chang, Tsai, Ching-Hui, Chen, Po-Hua, Lee, Yungling Leo, E-mail: leolee@ntu.edu.tw, & Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan. Smoking-related microRNAs and mRNAs in human peripheral blood mononuclear cells. United States. doi:10.1016/J.TAAP.2016.06.020.
Su, Ming-Wei, Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan, Yu, Sung-Liang, Lin, Wen-Chang, Tsai, Ching-Hui, Chen, Po-Hua, Lee, Yungling Leo, E-mail: leolee@ntu.edu.tw, and Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan. Mon . "Smoking-related microRNAs and mRNAs in human peripheral blood mononuclear cells". United States. doi:10.1016/J.TAAP.2016.06.020.
@article{osti_22689236,
title = {Smoking-related microRNAs and mRNAs in human peripheral blood mononuclear cells},
author = {Su, Ming-Wei and Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan and Yu, Sung-Liang and Lin, Wen-Chang and Tsai, Ching-Hui and Chen, Po-Hua and Lee, Yungling Leo, E-mail: leolee@ntu.edu.tw and Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan},
abstractNote = {Teenager smoking is of great importance in public health. Functional roles of microRNAs have been documented in smoke-induced gene expression changes, but comprehensive mechanisms of microRNA-mRNA regulation and benefits remained poorly understood. We conducted the Teenager Smoking Reduction Trial (TSRT) to investigate the causal association between active smoking reduction and whole-genome microRNA and mRNA expression changes in human peripheral blood mononuclear cells (PBMC). A total of 12 teenagers with a substantial reduction in smoke quantity and a decrease in urine cotinine/creatinine ratio were enrolled in genomic analyses. In Gene Set Enrichment Analysis (GSEA) and Ingenuity Pathway Analysis (IPA), differentially expressed genes altered by smoke reduction were mainly associated with glucocorticoid receptor signaling pathway. The integrative analysis of microRNA and mRNA found eleven differentially expressed microRNAs negatively correlated with predicted target genes. CD83 molecule regulated by miR-4498 in human PBMC, was critical for the canonical pathway of communication between innate and adaptive immune cells. Our data demonstrated that microRNAs could regulate immune responses in human PBMC after habitual smokers quit smoking and support the potential translational value of microRNAs in regulating disease-relevant gene expression caused by tobacco smoke. - Highlights: • We conducted a smoke reduction trial program and investigated the causal relationship between smoke and gene regulation. • MicroRNA and mRNA expression changes were examined in human PBMC. • MicroRNAs are important in regulating disease-causal genes after tobacco smoke reduction.},
doi = {10.1016/J.TAAP.2016.06.020},
journal = {Toxicology and Applied Pharmacology},
issn = {0041-008X},
number = ,
volume = 305,
place = {United States},
year = {2016},
month = {8}
}